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Reactive Oxygen Species in the Aorta and Perivascular Adipose Tissue Precedes Endothelial Dysfunction in the Aorta of Mice with a High-Fat High-Sucrose Diet and Additional Factors

Metabolic syndrome (Mets) is the major contributor to the onset of metabolic complications, such as hypertension, type 2 diabetes mellitus (DM), dyslipidemia, and non-alcoholic fatty liver disease, resulting in cardiovascular diseases. C57BL/6 mice on a high-fat and high-sucrose diet (HFHSD) are a w...

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Autores principales: Osaki, Ayumu, Kagami, Kazuki, Ishinoda, Yuki, Sato, Atsushi, Kimura, Toyokazu, Horii, Shunpei, Ito, Kei, Toya, Takumi, Ido, Yasuo, Namba, Takayuki, Masaki, Nobuyuki, Nagatomo, Yuji, Adachi, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095299/
https://www.ncbi.nlm.nih.gov/pubmed/37047458
http://dx.doi.org/10.3390/ijms24076486
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author Osaki, Ayumu
Kagami, Kazuki
Ishinoda, Yuki
Sato, Atsushi
Kimura, Toyokazu
Horii, Shunpei
Ito, Kei
Toya, Takumi
Ido, Yasuo
Namba, Takayuki
Masaki, Nobuyuki
Nagatomo, Yuji
Adachi, Takeshi
author_facet Osaki, Ayumu
Kagami, Kazuki
Ishinoda, Yuki
Sato, Atsushi
Kimura, Toyokazu
Horii, Shunpei
Ito, Kei
Toya, Takumi
Ido, Yasuo
Namba, Takayuki
Masaki, Nobuyuki
Nagatomo, Yuji
Adachi, Takeshi
author_sort Osaki, Ayumu
collection PubMed
description Metabolic syndrome (Mets) is the major contributor to the onset of metabolic complications, such as hypertension, type 2 diabetes mellitus (DM), dyslipidemia, and non-alcoholic fatty liver disease, resulting in cardiovascular diseases. C57BL/6 mice on a high-fat and high-sucrose diet (HFHSD) are a well-established model of Mets but have minor endothelial dysfunction in isolated aortas without perivascular adipose tissue (PVAT). The purpose of this study was to evaluate the effects of additional factors such as DM, dyslipidemia, and steatohepatitis on endothelial dysfunction in aortas without PVAT. Here, we employed eight-week-old male C57BL/6 mice fed with a normal diet (ND), HFHSD, steatohepatitis choline-deficient HFHSD (HFHSD-SH), and HFHSD containing 1% cholesterol and 0.1% deoxycholic acid (HFHSD-Chol) for 16 weeks. At week 20, some HFHSD-fed mice were treated with streptozocin to develop diabetes (HFHSD-DM). In PVAT-free aortas, the endothelial-dependent relaxation (EDR) did not differ between ND and HFHSD (p = 0.25), but in aortas with PVAT, the EDR of HFHSD-fed mice was impaired compared with ND-fed mice (p = 0.005). HFHSD-DM, HFHSD-SH, and HFHSD-Chol impaired the EDR in aortas without PVAT (p < 0.001, p = 0.019, and p = 0.009 vs. ND, respectively). Furthermore, tempol rescued the EDR in those models. In the Mets model, the EDR is compromised by PVAT, but with the addition of DM, dyslipidemia, and SH, the vessels themselves may result in impaired EDR.
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spelling pubmed-100952992023-04-13 Reactive Oxygen Species in the Aorta and Perivascular Adipose Tissue Precedes Endothelial Dysfunction in the Aorta of Mice with a High-Fat High-Sucrose Diet and Additional Factors Osaki, Ayumu Kagami, Kazuki Ishinoda, Yuki Sato, Atsushi Kimura, Toyokazu Horii, Shunpei Ito, Kei Toya, Takumi Ido, Yasuo Namba, Takayuki Masaki, Nobuyuki Nagatomo, Yuji Adachi, Takeshi Int J Mol Sci Article Metabolic syndrome (Mets) is the major contributor to the onset of metabolic complications, such as hypertension, type 2 diabetes mellitus (DM), dyslipidemia, and non-alcoholic fatty liver disease, resulting in cardiovascular diseases. C57BL/6 mice on a high-fat and high-sucrose diet (HFHSD) are a well-established model of Mets but have minor endothelial dysfunction in isolated aortas without perivascular adipose tissue (PVAT). The purpose of this study was to evaluate the effects of additional factors such as DM, dyslipidemia, and steatohepatitis on endothelial dysfunction in aortas without PVAT. Here, we employed eight-week-old male C57BL/6 mice fed with a normal diet (ND), HFHSD, steatohepatitis choline-deficient HFHSD (HFHSD-SH), and HFHSD containing 1% cholesterol and 0.1% deoxycholic acid (HFHSD-Chol) for 16 weeks. At week 20, some HFHSD-fed mice were treated with streptozocin to develop diabetes (HFHSD-DM). In PVAT-free aortas, the endothelial-dependent relaxation (EDR) did not differ between ND and HFHSD (p = 0.25), but in aortas with PVAT, the EDR of HFHSD-fed mice was impaired compared with ND-fed mice (p = 0.005). HFHSD-DM, HFHSD-SH, and HFHSD-Chol impaired the EDR in aortas without PVAT (p < 0.001, p = 0.019, and p = 0.009 vs. ND, respectively). Furthermore, tempol rescued the EDR in those models. In the Mets model, the EDR is compromised by PVAT, but with the addition of DM, dyslipidemia, and SH, the vessels themselves may result in impaired EDR. MDPI 2023-03-30 /pmc/articles/PMC10095299/ /pubmed/37047458 http://dx.doi.org/10.3390/ijms24076486 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Osaki, Ayumu
Kagami, Kazuki
Ishinoda, Yuki
Sato, Atsushi
Kimura, Toyokazu
Horii, Shunpei
Ito, Kei
Toya, Takumi
Ido, Yasuo
Namba, Takayuki
Masaki, Nobuyuki
Nagatomo, Yuji
Adachi, Takeshi
Reactive Oxygen Species in the Aorta and Perivascular Adipose Tissue Precedes Endothelial Dysfunction in the Aorta of Mice with a High-Fat High-Sucrose Diet and Additional Factors
title Reactive Oxygen Species in the Aorta and Perivascular Adipose Tissue Precedes Endothelial Dysfunction in the Aorta of Mice with a High-Fat High-Sucrose Diet and Additional Factors
title_full Reactive Oxygen Species in the Aorta and Perivascular Adipose Tissue Precedes Endothelial Dysfunction in the Aorta of Mice with a High-Fat High-Sucrose Diet and Additional Factors
title_fullStr Reactive Oxygen Species in the Aorta and Perivascular Adipose Tissue Precedes Endothelial Dysfunction in the Aorta of Mice with a High-Fat High-Sucrose Diet and Additional Factors
title_full_unstemmed Reactive Oxygen Species in the Aorta and Perivascular Adipose Tissue Precedes Endothelial Dysfunction in the Aorta of Mice with a High-Fat High-Sucrose Diet and Additional Factors
title_short Reactive Oxygen Species in the Aorta and Perivascular Adipose Tissue Precedes Endothelial Dysfunction in the Aorta of Mice with a High-Fat High-Sucrose Diet and Additional Factors
title_sort reactive oxygen species in the aorta and perivascular adipose tissue precedes endothelial dysfunction in the aorta of mice with a high-fat high-sucrose diet and additional factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095299/
https://www.ncbi.nlm.nih.gov/pubmed/37047458
http://dx.doi.org/10.3390/ijms24076486
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