Cargando…
Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study
Microbial contamination in the hospital environment is a major concern for public health, since it significantly contributes to the onset of healthcare-associated infections (HAIs), which are further complicated by the alarming level of antimicrobial resistance (AMR) of HAI-associated pathogens. Che...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095405/ https://www.ncbi.nlm.nih.gov/pubmed/37047510 http://dx.doi.org/10.3390/ijms24076535 |
_version_ | 1785024075588960256 |
---|---|
author | D’Accolti, Maria Soffritti, Irene Bini, Francesca Mazziga, Eleonora Arnoldo, Luca Volta, Antonella Bisi, Matteo Antonioli, Paola Laurenti, Patrizia Ricciardi, Walter Vincenti, Sara Mazzacane, Sante Caselli, Elisabetta |
author_facet | D’Accolti, Maria Soffritti, Irene Bini, Francesca Mazziga, Eleonora Arnoldo, Luca Volta, Antonella Bisi, Matteo Antonioli, Paola Laurenti, Patrizia Ricciardi, Walter Vincenti, Sara Mazzacane, Sante Caselli, Elisabetta |
author_sort | D’Accolti, Maria |
collection | PubMed |
description | Microbial contamination in the hospital environment is a major concern for public health, since it significantly contributes to the onset of healthcare-associated infections (HAIs), which are further complicated by the alarming level of antimicrobial resistance (AMR) of HAI-associated pathogens. Chemical disinfection to control bioburden has a temporary effect and can favor the selection of resistant pathogens, as observed during the COVID-19 pandemic. Instead, probiotic-based sanitation (probiotic cleaning hygiene system, PCHS) was reported to stably abate pathogens, AMR, and HAIs. PCHS action is not rapid nor specific, being based on competitive exclusion, but the addition of lytic bacteriophages that quickly and specifically kill selected bacteria was shown to improve PCHS effectiveness. This study aimed to investigate the effect of such combined probiotic–phage sanitation (PCHSφ) in two Italian hospitals, targeting staphylococcal contamination. The results showed that PCHSφ could provide a significantly higher removal of staphylococci, including resistant strains, compared with disinfectants (−76%, p < 0.05) and PCHS alone (−50%, p < 0.05). Extraordinary sporadic chlorine disinfection appeared compatible with PCHSφ, while frequent routine chlorine usage inactivated the probiotic/phage components, preventing PCHSφ action. The collected data highlight the potential of a biological sanitation for better control of the infectious risk in healthcare facilities, without worsening pollution and AMR concerns. |
format | Online Article Text |
id | pubmed-10095405 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100954052023-04-13 Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study D’Accolti, Maria Soffritti, Irene Bini, Francesca Mazziga, Eleonora Arnoldo, Luca Volta, Antonella Bisi, Matteo Antonioli, Paola Laurenti, Patrizia Ricciardi, Walter Vincenti, Sara Mazzacane, Sante Caselli, Elisabetta Int J Mol Sci Article Microbial contamination in the hospital environment is a major concern for public health, since it significantly contributes to the onset of healthcare-associated infections (HAIs), which are further complicated by the alarming level of antimicrobial resistance (AMR) of HAI-associated pathogens. Chemical disinfection to control bioburden has a temporary effect and can favor the selection of resistant pathogens, as observed during the COVID-19 pandemic. Instead, probiotic-based sanitation (probiotic cleaning hygiene system, PCHS) was reported to stably abate pathogens, AMR, and HAIs. PCHS action is not rapid nor specific, being based on competitive exclusion, but the addition of lytic bacteriophages that quickly and specifically kill selected bacteria was shown to improve PCHS effectiveness. This study aimed to investigate the effect of such combined probiotic–phage sanitation (PCHSφ) in two Italian hospitals, targeting staphylococcal contamination. The results showed that PCHSφ could provide a significantly higher removal of staphylococci, including resistant strains, compared with disinfectants (−76%, p < 0.05) and PCHS alone (−50%, p < 0.05). Extraordinary sporadic chlorine disinfection appeared compatible with PCHSφ, while frequent routine chlorine usage inactivated the probiotic/phage components, preventing PCHSφ action. The collected data highlight the potential of a biological sanitation for better control of the infectious risk in healthcare facilities, without worsening pollution and AMR concerns. MDPI 2023-03-31 /pmc/articles/PMC10095405/ /pubmed/37047510 http://dx.doi.org/10.3390/ijms24076535 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article D’Accolti, Maria Soffritti, Irene Bini, Francesca Mazziga, Eleonora Arnoldo, Luca Volta, Antonella Bisi, Matteo Antonioli, Paola Laurenti, Patrizia Ricciardi, Walter Vincenti, Sara Mazzacane, Sante Caselli, Elisabetta Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study |
title | Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study |
title_full | Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study |
title_fullStr | Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study |
title_full_unstemmed | Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study |
title_short | Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study |
title_sort | potential use of a combined bacteriophage–probiotic sanitation system to control microbial contamination and amr in healthcare settings: a pre-post intervention study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095405/ https://www.ncbi.nlm.nih.gov/pubmed/37047510 http://dx.doi.org/10.3390/ijms24076535 |
work_keys_str_mv | AT daccoltimaria potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT soffrittiirene potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT binifrancesca potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT mazzigaeleonora potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT arnoldoluca potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT voltaantonella potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT bisimatteo potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT antoniolipaola potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT laurentipatrizia potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT ricciardiwalter potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT vincentisara potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT mazzacanesante potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy AT casellielisabetta potentialuseofacombinedbacteriophageprobioticsanitationsystemtocontrolmicrobialcontaminationandamrinhealthcaresettingsaprepostinterventionstudy |