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Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study

Microbial contamination in the hospital environment is a major concern for public health, since it significantly contributes to the onset of healthcare-associated infections (HAIs), which are further complicated by the alarming level of antimicrobial resistance (AMR) of HAI-associated pathogens. Che...

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Autores principales: D’Accolti, Maria, Soffritti, Irene, Bini, Francesca, Mazziga, Eleonora, Arnoldo, Luca, Volta, Antonella, Bisi, Matteo, Antonioli, Paola, Laurenti, Patrizia, Ricciardi, Walter, Vincenti, Sara, Mazzacane, Sante, Caselli, Elisabetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095405/
https://www.ncbi.nlm.nih.gov/pubmed/37047510
http://dx.doi.org/10.3390/ijms24076535
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author D’Accolti, Maria
Soffritti, Irene
Bini, Francesca
Mazziga, Eleonora
Arnoldo, Luca
Volta, Antonella
Bisi, Matteo
Antonioli, Paola
Laurenti, Patrizia
Ricciardi, Walter
Vincenti, Sara
Mazzacane, Sante
Caselli, Elisabetta
author_facet D’Accolti, Maria
Soffritti, Irene
Bini, Francesca
Mazziga, Eleonora
Arnoldo, Luca
Volta, Antonella
Bisi, Matteo
Antonioli, Paola
Laurenti, Patrizia
Ricciardi, Walter
Vincenti, Sara
Mazzacane, Sante
Caselli, Elisabetta
author_sort D’Accolti, Maria
collection PubMed
description Microbial contamination in the hospital environment is a major concern for public health, since it significantly contributes to the onset of healthcare-associated infections (HAIs), which are further complicated by the alarming level of antimicrobial resistance (AMR) of HAI-associated pathogens. Chemical disinfection to control bioburden has a temporary effect and can favor the selection of resistant pathogens, as observed during the COVID-19 pandemic. Instead, probiotic-based sanitation (probiotic cleaning hygiene system, PCHS) was reported to stably abate pathogens, AMR, and HAIs. PCHS action is not rapid nor specific, being based on competitive exclusion, but the addition of lytic bacteriophages that quickly and specifically kill selected bacteria was shown to improve PCHS effectiveness. This study aimed to investigate the effect of such combined probiotic–phage sanitation (PCHSφ) in two Italian hospitals, targeting staphylococcal contamination. The results showed that PCHSφ could provide a significantly higher removal of staphylococci, including resistant strains, compared with disinfectants (−76%, p < 0.05) and PCHS alone (−50%, p < 0.05). Extraordinary sporadic chlorine disinfection appeared compatible with PCHSφ, while frequent routine chlorine usage inactivated the probiotic/phage components, preventing PCHSφ action. The collected data highlight the potential of a biological sanitation for better control of the infectious risk in healthcare facilities, without worsening pollution and AMR concerns.
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spelling pubmed-100954052023-04-13 Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study D’Accolti, Maria Soffritti, Irene Bini, Francesca Mazziga, Eleonora Arnoldo, Luca Volta, Antonella Bisi, Matteo Antonioli, Paola Laurenti, Patrizia Ricciardi, Walter Vincenti, Sara Mazzacane, Sante Caselli, Elisabetta Int J Mol Sci Article Microbial contamination in the hospital environment is a major concern for public health, since it significantly contributes to the onset of healthcare-associated infections (HAIs), which are further complicated by the alarming level of antimicrobial resistance (AMR) of HAI-associated pathogens. Chemical disinfection to control bioburden has a temporary effect and can favor the selection of resistant pathogens, as observed during the COVID-19 pandemic. Instead, probiotic-based sanitation (probiotic cleaning hygiene system, PCHS) was reported to stably abate pathogens, AMR, and HAIs. PCHS action is not rapid nor specific, being based on competitive exclusion, but the addition of lytic bacteriophages that quickly and specifically kill selected bacteria was shown to improve PCHS effectiveness. This study aimed to investigate the effect of such combined probiotic–phage sanitation (PCHSφ) in two Italian hospitals, targeting staphylococcal contamination. The results showed that PCHSφ could provide a significantly higher removal of staphylococci, including resistant strains, compared with disinfectants (−76%, p < 0.05) and PCHS alone (−50%, p < 0.05). Extraordinary sporadic chlorine disinfection appeared compatible with PCHSφ, while frequent routine chlorine usage inactivated the probiotic/phage components, preventing PCHSφ action. The collected data highlight the potential of a biological sanitation for better control of the infectious risk in healthcare facilities, without worsening pollution and AMR concerns. MDPI 2023-03-31 /pmc/articles/PMC10095405/ /pubmed/37047510 http://dx.doi.org/10.3390/ijms24076535 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
D’Accolti, Maria
Soffritti, Irene
Bini, Francesca
Mazziga, Eleonora
Arnoldo, Luca
Volta, Antonella
Bisi, Matteo
Antonioli, Paola
Laurenti, Patrizia
Ricciardi, Walter
Vincenti, Sara
Mazzacane, Sante
Caselli, Elisabetta
Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study
title Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study
title_full Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study
title_fullStr Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study
title_full_unstemmed Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study
title_short Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study
title_sort potential use of a combined bacteriophage–probiotic sanitation system to control microbial contamination and amr in healthcare settings: a pre-post intervention study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095405/
https://www.ncbi.nlm.nih.gov/pubmed/37047510
http://dx.doi.org/10.3390/ijms24076535
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