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Molecular prevalence and associated risk factors of Anaplasma ovis in Pakistani sheep

BACKGROUND: Majority of Pakistani population lives in rural areas and raising animals, especially the small ruminants, is their primary source of income. Anaplasma ovis is known to infect small ruminants globally and causing significant economic losses to livestock owners, however prevalence of Anap...

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Autores principales: Naeem, Muhammad, Amaro-Estrada, Itzel, Taqadus, Asia, Swelum, Ayman A., Alqhtani, Abdulmohsen H., Asif, Muhammad, Sajid, Muhammad, Khan, Asmat Ullah, Tariq, Aliza, Anjum, Summaya, Khan, Adil, Iqbal, Furhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095557/
https://www.ncbi.nlm.nih.gov/pubmed/37065244
http://dx.doi.org/10.3389/fvets.2023.1096418
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author Naeem, Muhammad
Amaro-Estrada, Itzel
Taqadus, Asia
Swelum, Ayman A.
Alqhtani, Abdulmohsen H.
Asif, Muhammad
Sajid, Muhammad
Khan, Asmat Ullah
Tariq, Aliza
Anjum, Summaya
Khan, Adil
Iqbal, Furhan
author_facet Naeem, Muhammad
Amaro-Estrada, Itzel
Taqadus, Asia
Swelum, Ayman A.
Alqhtani, Abdulmohsen H.
Asif, Muhammad
Sajid, Muhammad
Khan, Asmat Ullah
Tariq, Aliza
Anjum, Summaya
Khan, Adil
Iqbal, Furhan
author_sort Naeem, Muhammad
collection PubMed
description BACKGROUND: Majority of Pakistani population lives in rural areas and raising animals, especially the small ruminants, is their primary source of income. Anaplasma ovis is known to infect small ruminants globally and causing significant economic losses to livestock owners, however prevalence of Anaplasma ovis has been least investigated from Pakistan despite having a huge sheep population. METHODS: The present study was conducted from June 2021 till December 2021 to report the PCR based prevalence of Anaplasma ovis in the blood samples of sheep (n = 239) that were collected from District Dera Ghazi Khan in Pakistan. RESULTS: Out of 239 samples, 30 (12.5%) amplified a 347 bp fragment specific for the msp4 gene of Anaplasma ovis. Represented partial msp4 gene sequences were confirmed by Sanger sequencing and deposited to GenBank (OP620757-59). None of the studied epidemiological factors (age, sex, breed, size of herd, dogs with herd, and composition of herd) showed an association (P > 0.05) with the Anaplasma ovis infection in enrolled sheep. Analysis of the amplified partial mSP4 sequence of Anaplasma ovis revealed that this gene is highly conserved as all three sequences were identical and phylogenetically resembled with the msp4 sequences amplified from small ruminants in China, Kenya, and Germany, Turkey, Portugal, Tunisia and India. In conclusion, for the first time, we are reporting a moderate prevalence of Anaplasma ovis prevalence in Pakistani sheep and this data will help in developing the integrated control policies against this newly reported tick-borne disease that is infecting our sheep breeds.
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spelling pubmed-100955572023-04-13 Molecular prevalence and associated risk factors of Anaplasma ovis in Pakistani sheep Naeem, Muhammad Amaro-Estrada, Itzel Taqadus, Asia Swelum, Ayman A. Alqhtani, Abdulmohsen H. Asif, Muhammad Sajid, Muhammad Khan, Asmat Ullah Tariq, Aliza Anjum, Summaya Khan, Adil Iqbal, Furhan Front Vet Sci Veterinary Science BACKGROUND: Majority of Pakistani population lives in rural areas and raising animals, especially the small ruminants, is their primary source of income. Anaplasma ovis is known to infect small ruminants globally and causing significant economic losses to livestock owners, however prevalence of Anaplasma ovis has been least investigated from Pakistan despite having a huge sheep population. METHODS: The present study was conducted from June 2021 till December 2021 to report the PCR based prevalence of Anaplasma ovis in the blood samples of sheep (n = 239) that were collected from District Dera Ghazi Khan in Pakistan. RESULTS: Out of 239 samples, 30 (12.5%) amplified a 347 bp fragment specific for the msp4 gene of Anaplasma ovis. Represented partial msp4 gene sequences were confirmed by Sanger sequencing and deposited to GenBank (OP620757-59). None of the studied epidemiological factors (age, sex, breed, size of herd, dogs with herd, and composition of herd) showed an association (P > 0.05) with the Anaplasma ovis infection in enrolled sheep. Analysis of the amplified partial mSP4 sequence of Anaplasma ovis revealed that this gene is highly conserved as all three sequences were identical and phylogenetically resembled with the msp4 sequences amplified from small ruminants in China, Kenya, and Germany, Turkey, Portugal, Tunisia and India. In conclusion, for the first time, we are reporting a moderate prevalence of Anaplasma ovis prevalence in Pakistani sheep and this data will help in developing the integrated control policies against this newly reported tick-borne disease that is infecting our sheep breeds. Frontiers Media S.A. 2023-03-29 /pmc/articles/PMC10095557/ /pubmed/37065244 http://dx.doi.org/10.3389/fvets.2023.1096418 Text en Copyright © 2023 Naeem, Amaro-Estrada, Taqadus, Swelum, Alqhtani, Asif, Sajid, Khan, Tariq, Anjum, Khan and Iqbal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Naeem, Muhammad
Amaro-Estrada, Itzel
Taqadus, Asia
Swelum, Ayman A.
Alqhtani, Abdulmohsen H.
Asif, Muhammad
Sajid, Muhammad
Khan, Asmat Ullah
Tariq, Aliza
Anjum, Summaya
Khan, Adil
Iqbal, Furhan
Molecular prevalence and associated risk factors of Anaplasma ovis in Pakistani sheep
title Molecular prevalence and associated risk factors of Anaplasma ovis in Pakistani sheep
title_full Molecular prevalence and associated risk factors of Anaplasma ovis in Pakistani sheep
title_fullStr Molecular prevalence and associated risk factors of Anaplasma ovis in Pakistani sheep
title_full_unstemmed Molecular prevalence and associated risk factors of Anaplasma ovis in Pakistani sheep
title_short Molecular prevalence and associated risk factors of Anaplasma ovis in Pakistani sheep
title_sort molecular prevalence and associated risk factors of anaplasma ovis in pakistani sheep
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095557/
https://www.ncbi.nlm.nih.gov/pubmed/37065244
http://dx.doi.org/10.3389/fvets.2023.1096418
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