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Anti-SARS-CoV-2 Activity of Ampelozizyphus amazonicus (Saracura-Mirá): Focus on the Modulation of the Spike-ACE2 Interaction by Chemically Characterized Bark Extracts by LC-DAD-APCI-MS/MS

Traditional medicine shows several treatment protocols for COVID-19 based on natural products, revealing its potential as a possible source of anti-SARS-CoV-2 agents. Ampelozizyphus amazonicus is popularly used in the Brazilian Amazon as a fortifier and tonic, and recently, it has been reported to r...

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Autores principales: Campos, Mariana Freire, Mendonça, Simony Carvalho, Peñaloza, Evelyn Maribel Condori, de Oliveira, Beatriz A. C., Rosa, Alice S., Leitão, Gilda Guimarães, Tucci, Amanda R., Ferreira, Vivian Neuza S., Oliveira, Thamara Kelcya F., Miranda, Milene Dias, Allonso, Diego, Leitão, Suzana Guimarães
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095690/
https://www.ncbi.nlm.nih.gov/pubmed/37049921
http://dx.doi.org/10.3390/molecules28073159
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author Campos, Mariana Freire
Mendonça, Simony Carvalho
Peñaloza, Evelyn Maribel Condori
de Oliveira, Beatriz A. C.
Rosa, Alice S.
Leitão, Gilda Guimarães
Tucci, Amanda R.
Ferreira, Vivian Neuza S.
Oliveira, Thamara Kelcya F.
Miranda, Milene Dias
Allonso, Diego
Leitão, Suzana Guimarães
author_facet Campos, Mariana Freire
Mendonça, Simony Carvalho
Peñaloza, Evelyn Maribel Condori
de Oliveira, Beatriz A. C.
Rosa, Alice S.
Leitão, Gilda Guimarães
Tucci, Amanda R.
Ferreira, Vivian Neuza S.
Oliveira, Thamara Kelcya F.
Miranda, Milene Dias
Allonso, Diego
Leitão, Suzana Guimarães
author_sort Campos, Mariana Freire
collection PubMed
description Traditional medicine shows several treatment protocols for COVID-19 based on natural products, revealing its potential as a possible source of anti-SARS-CoV-2 agents. Ampelozizyphus amazonicus is popularly used in the Brazilian Amazon as a fortifier and tonic, and recently, it has been reported to relieve COVID-19 symptoms. This work aimed to investigate the antiviral potential of A. amazonicus, focusing on the inhibition of spike and ACE2 receptor interaction, a key step in successful infection. Although saponins are the major compounds of this plant and often reported as its active principles, a polyphenol-rich extract was the best inhibitor of the spike and ACE2 interaction. Chemical characterization of A. amazonicus bark extracts by LC-DAD-APCI-MS/MS before and after clean-up steps for polyphenol removal showed that the latter play an essential role in maintaining this activity. The effects of the extracts on viral replication were also assessed, and all samples (aqueous and ethanol extracts) demonstrated in vitro activity, inhibiting viral titers in the supernatant of Calu-3 cells after 24 hpi. By acting both in the SARS-CoV-2 cell entry process and its replication, A. amazonicus bark extracts stand out as a multitarget agent, highlighting the species as a promising candidate in the development of anti-SARS-CoV-2 drugs.
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spelling pubmed-100956902023-04-13 Anti-SARS-CoV-2 Activity of Ampelozizyphus amazonicus (Saracura-Mirá): Focus on the Modulation of the Spike-ACE2 Interaction by Chemically Characterized Bark Extracts by LC-DAD-APCI-MS/MS Campos, Mariana Freire Mendonça, Simony Carvalho Peñaloza, Evelyn Maribel Condori de Oliveira, Beatriz A. C. Rosa, Alice S. Leitão, Gilda Guimarães Tucci, Amanda R. Ferreira, Vivian Neuza S. Oliveira, Thamara Kelcya F. Miranda, Milene Dias Allonso, Diego Leitão, Suzana Guimarães Molecules Article Traditional medicine shows several treatment protocols for COVID-19 based on natural products, revealing its potential as a possible source of anti-SARS-CoV-2 agents. Ampelozizyphus amazonicus is popularly used in the Brazilian Amazon as a fortifier and tonic, and recently, it has been reported to relieve COVID-19 symptoms. This work aimed to investigate the antiviral potential of A. amazonicus, focusing on the inhibition of spike and ACE2 receptor interaction, a key step in successful infection. Although saponins are the major compounds of this plant and often reported as its active principles, a polyphenol-rich extract was the best inhibitor of the spike and ACE2 interaction. Chemical characterization of A. amazonicus bark extracts by LC-DAD-APCI-MS/MS before and after clean-up steps for polyphenol removal showed that the latter play an essential role in maintaining this activity. The effects of the extracts on viral replication were also assessed, and all samples (aqueous and ethanol extracts) demonstrated in vitro activity, inhibiting viral titers in the supernatant of Calu-3 cells after 24 hpi. By acting both in the SARS-CoV-2 cell entry process and its replication, A. amazonicus bark extracts stand out as a multitarget agent, highlighting the species as a promising candidate in the development of anti-SARS-CoV-2 drugs. MDPI 2023-04-01 /pmc/articles/PMC10095690/ /pubmed/37049921 http://dx.doi.org/10.3390/molecules28073159 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Campos, Mariana Freire
Mendonça, Simony Carvalho
Peñaloza, Evelyn Maribel Condori
de Oliveira, Beatriz A. C.
Rosa, Alice S.
Leitão, Gilda Guimarães
Tucci, Amanda R.
Ferreira, Vivian Neuza S.
Oliveira, Thamara Kelcya F.
Miranda, Milene Dias
Allonso, Diego
Leitão, Suzana Guimarães
Anti-SARS-CoV-2 Activity of Ampelozizyphus amazonicus (Saracura-Mirá): Focus on the Modulation of the Spike-ACE2 Interaction by Chemically Characterized Bark Extracts by LC-DAD-APCI-MS/MS
title Anti-SARS-CoV-2 Activity of Ampelozizyphus amazonicus (Saracura-Mirá): Focus on the Modulation of the Spike-ACE2 Interaction by Chemically Characterized Bark Extracts by LC-DAD-APCI-MS/MS
title_full Anti-SARS-CoV-2 Activity of Ampelozizyphus amazonicus (Saracura-Mirá): Focus on the Modulation of the Spike-ACE2 Interaction by Chemically Characterized Bark Extracts by LC-DAD-APCI-MS/MS
title_fullStr Anti-SARS-CoV-2 Activity of Ampelozizyphus amazonicus (Saracura-Mirá): Focus on the Modulation of the Spike-ACE2 Interaction by Chemically Characterized Bark Extracts by LC-DAD-APCI-MS/MS
title_full_unstemmed Anti-SARS-CoV-2 Activity of Ampelozizyphus amazonicus (Saracura-Mirá): Focus on the Modulation of the Spike-ACE2 Interaction by Chemically Characterized Bark Extracts by LC-DAD-APCI-MS/MS
title_short Anti-SARS-CoV-2 Activity of Ampelozizyphus amazonicus (Saracura-Mirá): Focus on the Modulation of the Spike-ACE2 Interaction by Chemically Characterized Bark Extracts by LC-DAD-APCI-MS/MS
title_sort anti-sars-cov-2 activity of ampelozizyphus amazonicus (saracura-mirá): focus on the modulation of the spike-ace2 interaction by chemically characterized bark extracts by lc-dad-apci-ms/ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095690/
https://www.ncbi.nlm.nih.gov/pubmed/37049921
http://dx.doi.org/10.3390/molecules28073159
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