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A hybrid model connecting regulatory interactions with stem cell divisions in the root

Stem cells give rise to the entirety of cells within an organ. Maintaining stem cell identity and coordinately regulating stem cell divisions is crucial for proper development. In plants, mobile proteins, such as WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and SHORTROOT (SHR), regulate divisions in the root s...

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Autores principales: Van den Broeck, Lisa, Spurney, Ryan J., Fisher, Adam P., Schwartz, Michael, Clark, Natalie M., Nguyen, Thomas T., Madison, Imani, Gobble, Mariah, Long, Terri, Sozzani, Rosangela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095808/
https://www.ncbi.nlm.nih.gov/pubmed/37077208
http://dx.doi.org/10.1017/qpb.2021.1
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author Van den Broeck, Lisa
Spurney, Ryan J.
Fisher, Adam P.
Schwartz, Michael
Clark, Natalie M.
Nguyen, Thomas T.
Madison, Imani
Gobble, Mariah
Long, Terri
Sozzani, Rosangela
author_facet Van den Broeck, Lisa
Spurney, Ryan J.
Fisher, Adam P.
Schwartz, Michael
Clark, Natalie M.
Nguyen, Thomas T.
Madison, Imani
Gobble, Mariah
Long, Terri
Sozzani, Rosangela
author_sort Van den Broeck, Lisa
collection PubMed
description Stem cells give rise to the entirety of cells within an organ. Maintaining stem cell identity and coordinately regulating stem cell divisions is crucial for proper development. In plants, mobile proteins, such as WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and SHORTROOT (SHR), regulate divisions in the root stem cell niche. However, how these proteins coordinately function to establish systemic behaviour is not well understood. We propose a non-cell autonomous role for WOX5 in the cortex endodermis initial (CEI) and identify a regulator, ANGUSTIFOLIA (AN3)/GRF-INTERACTING FACTOR 1, that coordinates CEI divisions. Here, we show with a multi-scale hybrid model integrating ordinary differential equations (ODEs) and agent-based modeling that quiescent center (QC) and CEI divisions have different dynamics. Specifically, by combining continuous models to describe regulatory networks and agent-based rules, we model systemic behaviour, which led us to predict cell-type-specific expression dynamics of SHR, SCARECROW, WOX5, AN3 and CYCLIND6;1, and experimentally validate CEI cell divisions. Conclusively, our results show an interdependency between CEI and QC divisions.
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spelling pubmed-100958082023-04-18 A hybrid model connecting regulatory interactions with stem cell divisions in the root Van den Broeck, Lisa Spurney, Ryan J. Fisher, Adam P. Schwartz, Michael Clark, Natalie M. Nguyen, Thomas T. Madison, Imani Gobble, Mariah Long, Terri Sozzani, Rosangela Quant Plant Biol Original Research Article Stem cells give rise to the entirety of cells within an organ. Maintaining stem cell identity and coordinately regulating stem cell divisions is crucial for proper development. In plants, mobile proteins, such as WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and SHORTROOT (SHR), regulate divisions in the root stem cell niche. However, how these proteins coordinately function to establish systemic behaviour is not well understood. We propose a non-cell autonomous role for WOX5 in the cortex endodermis initial (CEI) and identify a regulator, ANGUSTIFOLIA (AN3)/GRF-INTERACTING FACTOR 1, that coordinates CEI divisions. Here, we show with a multi-scale hybrid model integrating ordinary differential equations (ODEs) and agent-based modeling that quiescent center (QC) and CEI divisions have different dynamics. Specifically, by combining continuous models to describe regulatory networks and agent-based rules, we model systemic behaviour, which led us to predict cell-type-specific expression dynamics of SHR, SCARECROW, WOX5, AN3 and CYCLIND6;1, and experimentally validate CEI cell divisions. Conclusively, our results show an interdependency between CEI and QC divisions. Cambridge University Press 2021-04-12 /pmc/articles/PMC10095808/ /pubmed/37077208 http://dx.doi.org/10.1017/qpb.2021.1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Article
Van den Broeck, Lisa
Spurney, Ryan J.
Fisher, Adam P.
Schwartz, Michael
Clark, Natalie M.
Nguyen, Thomas T.
Madison, Imani
Gobble, Mariah
Long, Terri
Sozzani, Rosangela
A hybrid model connecting regulatory interactions with stem cell divisions in the root
title A hybrid model connecting regulatory interactions with stem cell divisions in the root
title_full A hybrid model connecting regulatory interactions with stem cell divisions in the root
title_fullStr A hybrid model connecting regulatory interactions with stem cell divisions in the root
title_full_unstemmed A hybrid model connecting regulatory interactions with stem cell divisions in the root
title_short A hybrid model connecting regulatory interactions with stem cell divisions in the root
title_sort hybrid model connecting regulatory interactions with stem cell divisions in the root
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095808/
https://www.ncbi.nlm.nih.gov/pubmed/37077208
http://dx.doi.org/10.1017/qpb.2021.1
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