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Dynamic apico-basal enrichment of the F-actin during cytokinesis in Arabidopsis cells embedded in their tissues

Cell division is a tightly regulated mechanism, notably in tissues where malfunctions can lead to tumour formation or developmental defects. This is particularly true in land plants, where cells cannot relocate and therefore cytokinesis determines tissue topology. In plants, cell division is execute...

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Autores principales: Lebecq, Alexis, Fangain, Aurélie, Boussaroque, Alice, Caillaud, Marie-Cécile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095810/
https://www.ncbi.nlm.nih.gov/pubmed/37077960
http://dx.doi.org/10.1017/qpb.2022.1
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author Lebecq, Alexis
Fangain, Aurélie
Boussaroque, Alice
Caillaud, Marie-Cécile
author_facet Lebecq, Alexis
Fangain, Aurélie
Boussaroque, Alice
Caillaud, Marie-Cécile
author_sort Lebecq, Alexis
collection PubMed
description Cell division is a tightly regulated mechanism, notably in tissues where malfunctions can lead to tumour formation or developmental defects. This is particularly true in land plants, where cells cannot relocate and therefore cytokinesis determines tissue topology. In plants, cell division is executed in radically different manners than in animals, with the appearance of new structures and the disappearance of ancestral mechanisms. Whilst F-actin and microtubules closely co-exist, recent studies mainly focused on the involvement of microtubules in this key process. Here, we used a root tracking system to image the spatio-temporal dynamics of both F-actin reporters and cell division markers in dividing cells embedded in their tissues. In addition to the F-actin accumulation at the phragmoplast, we observed and quantified a dynamic apico-basal enrichment of F-actin from the prophase/metaphase transition until the end of the cytokinesis.
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spelling pubmed-100958102023-04-18 Dynamic apico-basal enrichment of the F-actin during cytokinesis in Arabidopsis cells embedded in their tissues Lebecq, Alexis Fangain, Aurélie Boussaroque, Alice Caillaud, Marie-Cécile Quant Plant Biol Original Research Article Cell division is a tightly regulated mechanism, notably in tissues where malfunctions can lead to tumour formation or developmental defects. This is particularly true in land plants, where cells cannot relocate and therefore cytokinesis determines tissue topology. In plants, cell division is executed in radically different manners than in animals, with the appearance of new structures and the disappearance of ancestral mechanisms. Whilst F-actin and microtubules closely co-exist, recent studies mainly focused on the involvement of microtubules in this key process. Here, we used a root tracking system to image the spatio-temporal dynamics of both F-actin reporters and cell division markers in dividing cells embedded in their tissues. In addition to the F-actin accumulation at the phragmoplast, we observed and quantified a dynamic apico-basal enrichment of F-actin from the prophase/metaphase transition until the end of the cytokinesis. Cambridge University Press 2022-02-15 /pmc/articles/PMC10095810/ /pubmed/37077960 http://dx.doi.org/10.1017/qpb.2022.1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is unaltered and is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use or in order to create a derivative work.
spellingShingle Original Research Article
Lebecq, Alexis
Fangain, Aurélie
Boussaroque, Alice
Caillaud, Marie-Cécile
Dynamic apico-basal enrichment of the F-actin during cytokinesis in Arabidopsis cells embedded in their tissues
title Dynamic apico-basal enrichment of the F-actin during cytokinesis in Arabidopsis cells embedded in their tissues
title_full Dynamic apico-basal enrichment of the F-actin during cytokinesis in Arabidopsis cells embedded in their tissues
title_fullStr Dynamic apico-basal enrichment of the F-actin during cytokinesis in Arabidopsis cells embedded in their tissues
title_full_unstemmed Dynamic apico-basal enrichment of the F-actin during cytokinesis in Arabidopsis cells embedded in their tissues
title_short Dynamic apico-basal enrichment of the F-actin during cytokinesis in Arabidopsis cells embedded in their tissues
title_sort dynamic apico-basal enrichment of the f-actin during cytokinesis in arabidopsis cells embedded in their tissues
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095810/
https://www.ncbi.nlm.nih.gov/pubmed/37077960
http://dx.doi.org/10.1017/qpb.2022.1
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