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Focus on biosensors: Looking through the lens of quantitative biology
In recent years, plant biologists interested in quantifying molecules and molecular events in vivo have started to complement reporter systems with genetically encoded fluorescent biosensors (GEFBs) that directly sense an analyte. Such biosensors can allow measurements at the level of individual cel...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cambridge University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095858/ https://www.ncbi.nlm.nih.gov/pubmed/37077214 http://dx.doi.org/10.1017/qpb.2021.10 |
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author | Rowe, James H. Jones, Alexander M. |
author_facet | Rowe, James H. Jones, Alexander M. |
author_sort | Rowe, James H. |
collection | PubMed |
description | In recent years, plant biologists interested in quantifying molecules and molecular events in vivo have started to complement reporter systems with genetically encoded fluorescent biosensors (GEFBs) that directly sense an analyte. Such biosensors can allow measurements at the level of individual cells and over time. This information is proving valuable to mathematical modellers interested in representing biological phenomena in silico, because improved measurements can guide improved model construction and model parametrisation. Advances in synthetic biology have accelerated the pace of biosensor development, and the simultaneous expression of spectrally compatible biosensors now allows quantification of multiple nodes in signalling networks. For biosensors that directly respond to stimuli, targeting to specific cellular compartments allows the observation of differential accumulation of analytes in distinct organelles, bringing insights to reactive oxygen species/calcium signalling and photosynthesis research. In conjunction with improved image analysis methods, advances in biosensor imaging can help close the loop between experimentation and mathematical modelling. |
format | Online Article Text |
id | pubmed-10095858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Cambridge University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-100958582023-04-18 Focus on biosensors: Looking through the lens of quantitative biology Rowe, James H. Jones, Alexander M. Quant Plant Biol Review In recent years, plant biologists interested in quantifying molecules and molecular events in vivo have started to complement reporter systems with genetically encoded fluorescent biosensors (GEFBs) that directly sense an analyte. Such biosensors can allow measurements at the level of individual cells and over time. This information is proving valuable to mathematical modellers interested in representing biological phenomena in silico, because improved measurements can guide improved model construction and model parametrisation. Advances in synthetic biology have accelerated the pace of biosensor development, and the simultaneous expression of spectrally compatible biosensors now allows quantification of multiple nodes in signalling networks. For biosensors that directly respond to stimuli, targeting to specific cellular compartments allows the observation of differential accumulation of analytes in distinct organelles, bringing insights to reactive oxygen species/calcium signalling and photosynthesis research. In conjunction with improved image analysis methods, advances in biosensor imaging can help close the loop between experimentation and mathematical modelling. Cambridge University Press 2021-10-12 /pmc/articles/PMC10095858/ /pubmed/37077214 http://dx.doi.org/10.1017/qpb.2021.10 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Rowe, James H. Jones, Alexander M. Focus on biosensors: Looking through the lens of quantitative biology |
title | Focus on biosensors: Looking through the lens of quantitative biology |
title_full | Focus on biosensors: Looking through the lens of quantitative biology |
title_fullStr | Focus on biosensors: Looking through the lens of quantitative biology |
title_full_unstemmed | Focus on biosensors: Looking through the lens of quantitative biology |
title_short | Focus on biosensors: Looking through the lens of quantitative biology |
title_sort | focus on biosensors: looking through the lens of quantitative biology |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095858/ https://www.ncbi.nlm.nih.gov/pubmed/37077214 http://dx.doi.org/10.1017/qpb.2021.10 |
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