Cargando…
Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells
The health benefits of wheat-derived arabinoxylan, a commonly consumed dietary fiber, have been studied for decades. However, its effect on the gut microenvironment and inflammatory bowel disease remains unclear. The objective of this study was to understand the effect of wheat-derived arabinoxylan...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096111/ https://www.ncbi.nlm.nih.gov/pubmed/37049841 http://dx.doi.org/10.3390/molecules28073079 |
_version_ | 1785024245463515136 |
---|---|
author | Chudan, Seita Ishibashi, Riko Nishikawa, Miyu Tabuchi, Yoshiaki Nagai, Yoshinori Ikushiro, Shinichi Furusawa, Yukihiro |
author_facet | Chudan, Seita Ishibashi, Riko Nishikawa, Miyu Tabuchi, Yoshiaki Nagai, Yoshinori Ikushiro, Shinichi Furusawa, Yukihiro |
author_sort | Chudan, Seita |
collection | PubMed |
description | The health benefits of wheat-derived arabinoxylan, a commonly consumed dietary fiber, have been studied for decades. However, its effect on the gut microenvironment and inflammatory bowel disease remains unclear. The objective of this study was to understand the effect of wheat-derived arabinoxylan on gut microbiota, colonic regulatory T cells (Tregs), and experimental colitis. In this study, healthy and chronic colitis model mice were fed chow containing cellulose or wheat-derived arabinoxylan for 2–6 weeks and subjected to subsequent analysis. A 16S-based metagenomic analysis of the fecal DNA revealed that Lachnospiraceae, comprising butyrate-producing and Treg-inducing bacteria, were overrepresented in arabinoxylan-fed mice. In line with the changes in the gut microbiota, both the fecal butyrate concentration and the colonic Treg population were elevated in the arabinoxylan-fed mice. In a T cell transfer model of chronic colitis, wheat-derived arabinoxylan ameliorated body weight loss and colonic tissue inflammation, which may, in part, be mediated by Treg induction. Moreover, wheat-derived arabinoxylan suppressed TNFα production from type 1 helper T cells in this colitis model. In conclusion, wheat-derived arabinoxylans, by altering the gut microenvironment, may be a promising prebiotic for the prevention of colitis. |
format | Online Article Text |
id | pubmed-10096111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100961112023-04-13 Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells Chudan, Seita Ishibashi, Riko Nishikawa, Miyu Tabuchi, Yoshiaki Nagai, Yoshinori Ikushiro, Shinichi Furusawa, Yukihiro Molecules Article The health benefits of wheat-derived arabinoxylan, a commonly consumed dietary fiber, have been studied for decades. However, its effect on the gut microenvironment and inflammatory bowel disease remains unclear. The objective of this study was to understand the effect of wheat-derived arabinoxylan on gut microbiota, colonic regulatory T cells (Tregs), and experimental colitis. In this study, healthy and chronic colitis model mice were fed chow containing cellulose or wheat-derived arabinoxylan for 2–6 weeks and subjected to subsequent analysis. A 16S-based metagenomic analysis of the fecal DNA revealed that Lachnospiraceae, comprising butyrate-producing and Treg-inducing bacteria, were overrepresented in arabinoxylan-fed mice. In line with the changes in the gut microbiota, both the fecal butyrate concentration and the colonic Treg population were elevated in the arabinoxylan-fed mice. In a T cell transfer model of chronic colitis, wheat-derived arabinoxylan ameliorated body weight loss and colonic tissue inflammation, which may, in part, be mediated by Treg induction. Moreover, wheat-derived arabinoxylan suppressed TNFα production from type 1 helper T cells in this colitis model. In conclusion, wheat-derived arabinoxylans, by altering the gut microenvironment, may be a promising prebiotic for the prevention of colitis. MDPI 2023-03-30 /pmc/articles/PMC10096111/ /pubmed/37049841 http://dx.doi.org/10.3390/molecules28073079 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chudan, Seita Ishibashi, Riko Nishikawa, Miyu Tabuchi, Yoshiaki Nagai, Yoshinori Ikushiro, Shinichi Furusawa, Yukihiro Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells |
title | Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells |
title_full | Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells |
title_fullStr | Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells |
title_full_unstemmed | Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells |
title_short | Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells |
title_sort | effect of wheat-derived arabinoxylan on the gut microbiota composition and colonic regulatory t cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096111/ https://www.ncbi.nlm.nih.gov/pubmed/37049841 http://dx.doi.org/10.3390/molecules28073079 |
work_keys_str_mv | AT chudanseita effectofwheatderivedarabinoxylanonthegutmicrobiotacompositionandcolonicregulatorytcells AT ishibashiriko effectofwheatderivedarabinoxylanonthegutmicrobiotacompositionandcolonicregulatorytcells AT nishikawamiyu effectofwheatderivedarabinoxylanonthegutmicrobiotacompositionandcolonicregulatorytcells AT tabuchiyoshiaki effectofwheatderivedarabinoxylanonthegutmicrobiotacompositionandcolonicregulatorytcells AT nagaiyoshinori effectofwheatderivedarabinoxylanonthegutmicrobiotacompositionandcolonicregulatorytcells AT ikushiroshinichi effectofwheatderivedarabinoxylanonthegutmicrobiotacompositionandcolonicregulatorytcells AT furusawayukihiro effectofwheatderivedarabinoxylanonthegutmicrobiotacompositionandcolonicregulatorytcells |