Cargando…
Protective Effects of Omega-3 Supplementation against Doxorubicin-Induced Deleterious Effects on the Liver and Kidneys of Rats
Anthracycline doxorubicin (DOX) is still widely used as a chemotherapeutic drug for some solid tumors. Although DOX is highly effective, its side effects are limiting factors, such as cardio, nephro and hepatotoxicity. As such, approaches used to mitigate these adverse effects are highly encouraged....
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096317/ https://www.ncbi.nlm.nih.gov/pubmed/37049766 http://dx.doi.org/10.3390/molecules28073004 |
_version_ | 1785024307168018432 |
---|---|
author | Espírito Santo, Sara Gomes Monte, Marina Gaiato Polegato, Bertha Furlan Barbisan, Luís Fernando Romualdo, Guilherme Ribeiro |
author_facet | Espírito Santo, Sara Gomes Monte, Marina Gaiato Polegato, Bertha Furlan Barbisan, Luís Fernando Romualdo, Guilherme Ribeiro |
author_sort | Espírito Santo, Sara Gomes |
collection | PubMed |
description | Anthracycline doxorubicin (DOX) is still widely used as a chemotherapeutic drug for some solid tumors. Although DOX is highly effective, its side effects are limiting factors, such as cardio, nephro and hepatotoxicity. As such, approaches used to mitigate these adverse effects are highly encouraged. Omega 3 (ω-3), which is a class of long-chain polyunsaturated fatty acids, has been shown to have anti-inflammatory and antioxidant effects in preclinical bioassays. Thus, we evaluated the protective effects of ω-3 supplementation on hepatotoxicity and nephrotoxicity induced by multiple DOX administrations in rodents. Male Wistar rats (10 rats/group) were treated daily with ω-3 (400 mg/kg/day) by gavage for six weeks. Two weeks after the first ω-3 administration, the rats received DOX (3.5 mg/kg, intraperitoneal, 1×/week) for four weeks. DOX treatment reduced body weight gain increased systemic genotoxicity and caused liver-related (increase in serum ALT levels, thickness of the Glisson’s capsule, compensatory proliferation and p65 levels) and kidney-related (increase in serum urea and creatinine levels, and incidence of tubular dilatation) deleterious outcomes. In contrast, ω-3 supplementation was safe and abrogated the DOX-related enhancement of systemic genotoxicity, serum urea and creatinine levels. Furthermore, ω-3 intervention reduced by 50% the incidence of kidney histological lesions while reducing by 40–50% the p65 protein level, and the proliferative response in the liver induced by DOX. Our findings indicate that ω-3 intervention attenuated the DOX-induced deleterious effects in the liver and kidney. Therefore, our findings may inspire future mechanistical investigations and clinical interventions with ω-3 on the reported outcomes. |
format | Online Article Text |
id | pubmed-10096317 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100963172023-04-13 Protective Effects of Omega-3 Supplementation against Doxorubicin-Induced Deleterious Effects on the Liver and Kidneys of Rats Espírito Santo, Sara Gomes Monte, Marina Gaiato Polegato, Bertha Furlan Barbisan, Luís Fernando Romualdo, Guilherme Ribeiro Molecules Article Anthracycline doxorubicin (DOX) is still widely used as a chemotherapeutic drug for some solid tumors. Although DOX is highly effective, its side effects are limiting factors, such as cardio, nephro and hepatotoxicity. As such, approaches used to mitigate these adverse effects are highly encouraged. Omega 3 (ω-3), which is a class of long-chain polyunsaturated fatty acids, has been shown to have anti-inflammatory and antioxidant effects in preclinical bioassays. Thus, we evaluated the protective effects of ω-3 supplementation on hepatotoxicity and nephrotoxicity induced by multiple DOX administrations in rodents. Male Wistar rats (10 rats/group) were treated daily with ω-3 (400 mg/kg/day) by gavage for six weeks. Two weeks after the first ω-3 administration, the rats received DOX (3.5 mg/kg, intraperitoneal, 1×/week) for four weeks. DOX treatment reduced body weight gain increased systemic genotoxicity and caused liver-related (increase in serum ALT levels, thickness of the Glisson’s capsule, compensatory proliferation and p65 levels) and kidney-related (increase in serum urea and creatinine levels, and incidence of tubular dilatation) deleterious outcomes. In contrast, ω-3 supplementation was safe and abrogated the DOX-related enhancement of systemic genotoxicity, serum urea and creatinine levels. Furthermore, ω-3 intervention reduced by 50% the incidence of kidney histological lesions while reducing by 40–50% the p65 protein level, and the proliferative response in the liver induced by DOX. Our findings indicate that ω-3 intervention attenuated the DOX-induced deleterious effects in the liver and kidney. Therefore, our findings may inspire future mechanistical investigations and clinical interventions with ω-3 on the reported outcomes. MDPI 2023-03-28 /pmc/articles/PMC10096317/ /pubmed/37049766 http://dx.doi.org/10.3390/molecules28073004 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Espírito Santo, Sara Gomes Monte, Marina Gaiato Polegato, Bertha Furlan Barbisan, Luís Fernando Romualdo, Guilherme Ribeiro Protective Effects of Omega-3 Supplementation against Doxorubicin-Induced Deleterious Effects on the Liver and Kidneys of Rats |
title | Protective Effects of Omega-3 Supplementation against Doxorubicin-Induced Deleterious Effects on the Liver and Kidneys of Rats |
title_full | Protective Effects of Omega-3 Supplementation against Doxorubicin-Induced Deleterious Effects on the Liver and Kidneys of Rats |
title_fullStr | Protective Effects of Omega-3 Supplementation against Doxorubicin-Induced Deleterious Effects on the Liver and Kidneys of Rats |
title_full_unstemmed | Protective Effects of Omega-3 Supplementation against Doxorubicin-Induced Deleterious Effects on the Liver and Kidneys of Rats |
title_short | Protective Effects of Omega-3 Supplementation against Doxorubicin-Induced Deleterious Effects on the Liver and Kidneys of Rats |
title_sort | protective effects of omega-3 supplementation against doxorubicin-induced deleterious effects on the liver and kidneys of rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096317/ https://www.ncbi.nlm.nih.gov/pubmed/37049766 http://dx.doi.org/10.3390/molecules28073004 |
work_keys_str_mv | AT espiritosantosaragomes protectiveeffectsofomega3supplementationagainstdoxorubicininduceddeleteriouseffectsontheliverandkidneysofrats AT montemarinagaiato protectiveeffectsofomega3supplementationagainstdoxorubicininduceddeleteriouseffectsontheliverandkidneysofrats AT polegatoberthafurlan protectiveeffectsofomega3supplementationagainstdoxorubicininduceddeleteriouseffectsontheliverandkidneysofrats AT barbisanluisfernando protectiveeffectsofomega3supplementationagainstdoxorubicininduceddeleteriouseffectsontheliverandkidneysofrats AT romualdoguilhermeribeiro protectiveeffectsofomega3supplementationagainstdoxorubicininduceddeleteriouseffectsontheliverandkidneysofrats |