Cargando…
Development and In Vitro Characterization of Antibiotic-Loaded Nanocarriers for Dental Delivery
The aim of this research work was to formulate and evaluate ciprofloxacin hydrochloride-loaded nanocarriers for treating dental infections and bone regeneration. Periodontal infection is associated with inflammation, soft tissue destruction, and bone loss. The objective of the study was to extract β...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096469/ https://www.ncbi.nlm.nih.gov/pubmed/37049683 http://dx.doi.org/10.3390/molecules28072914 |
_version_ | 1785024343897538560 |
---|---|
author | Ghazwani, Mohammed Vasudevan, Rajalakshimi Kandasamy, Geetha Hani, Umme Niharika, Gaddam Naredla, Manusri Devanandan, Praveen Puvvada, Ranadheer Chowdary Almehizia, Abdulrahman A. Hakami, Abdulrahim R. Dhurke, Rajeshri |
author_facet | Ghazwani, Mohammed Vasudevan, Rajalakshimi Kandasamy, Geetha Hani, Umme Niharika, Gaddam Naredla, Manusri Devanandan, Praveen Puvvada, Ranadheer Chowdary Almehizia, Abdulrahman A. Hakami, Abdulrahim R. Dhurke, Rajeshri |
author_sort | Ghazwani, Mohammed |
collection | PubMed |
description | The aim of this research work was to formulate and evaluate ciprofloxacin hydrochloride-loaded nanocarriers for treating dental infections and bone regeneration. Periodontal infection is associated with inflammation, soft tissue destruction, and bone loss. The objective of the study was to extract β tricalcium phosphate (β-TCP) from coral beach sand using the hydrothermal conversion method and load these nanocarriers with ciprofloxacin hydrochloride. The developed drug-loaded nanocarriers were evaluated for various parameters. In vitro drug-loading studies showed the highest drug loading of 71% for F1 with a drug: carrier ratio compared to plain ciprofloxacin hydrochloride gel. β-TCP and nanocarriers were evaluated for powder characteristics and the results were found to have excellent and fair flowability. In vitro drug release studies conducted over a period of 5 days confirmed the percentage drug release of 96% at the end of 120 h. Nanocarriers were found to be effective against S. aureus and E. coli showing statistically significant antibacterial activity at (* p < 0.05) significant level as compared to plain ciprofloxacin hydrochloride gel. The particle size of β-TCP and nanocarriers was found to be 2 µm. Fourier transform infra-red studies showed good compatibility between the drug and the excipients. Differential scanning calorimetry studies revealed the amorphous nature of the nanocarriers as evident from the peak shift. It is obvious from the XRD studies that the phase intensity was reduced, which demonstrates a decrease in crystallinity. Nanocarriers released the drug in a controlled manner, hence may prove to be a better option to treat dental caries as compared to conventional treatments. |
format | Online Article Text |
id | pubmed-10096469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100964692023-04-13 Development and In Vitro Characterization of Antibiotic-Loaded Nanocarriers for Dental Delivery Ghazwani, Mohammed Vasudevan, Rajalakshimi Kandasamy, Geetha Hani, Umme Niharika, Gaddam Naredla, Manusri Devanandan, Praveen Puvvada, Ranadheer Chowdary Almehizia, Abdulrahman A. Hakami, Abdulrahim R. Dhurke, Rajeshri Molecules Article The aim of this research work was to formulate and evaluate ciprofloxacin hydrochloride-loaded nanocarriers for treating dental infections and bone regeneration. Periodontal infection is associated with inflammation, soft tissue destruction, and bone loss. The objective of the study was to extract β tricalcium phosphate (β-TCP) from coral beach sand using the hydrothermal conversion method and load these nanocarriers with ciprofloxacin hydrochloride. The developed drug-loaded nanocarriers were evaluated for various parameters. In vitro drug-loading studies showed the highest drug loading of 71% for F1 with a drug: carrier ratio compared to plain ciprofloxacin hydrochloride gel. β-TCP and nanocarriers were evaluated for powder characteristics and the results were found to have excellent and fair flowability. In vitro drug release studies conducted over a period of 5 days confirmed the percentage drug release of 96% at the end of 120 h. Nanocarriers were found to be effective against S. aureus and E. coli showing statistically significant antibacterial activity at (* p < 0.05) significant level as compared to plain ciprofloxacin hydrochloride gel. The particle size of β-TCP and nanocarriers was found to be 2 µm. Fourier transform infra-red studies showed good compatibility between the drug and the excipients. Differential scanning calorimetry studies revealed the amorphous nature of the nanocarriers as evident from the peak shift. It is obvious from the XRD studies that the phase intensity was reduced, which demonstrates a decrease in crystallinity. Nanocarriers released the drug in a controlled manner, hence may prove to be a better option to treat dental caries as compared to conventional treatments. MDPI 2023-03-24 /pmc/articles/PMC10096469/ /pubmed/37049683 http://dx.doi.org/10.3390/molecules28072914 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ghazwani, Mohammed Vasudevan, Rajalakshimi Kandasamy, Geetha Hani, Umme Niharika, Gaddam Naredla, Manusri Devanandan, Praveen Puvvada, Ranadheer Chowdary Almehizia, Abdulrahman A. Hakami, Abdulrahim R. Dhurke, Rajeshri Development and In Vitro Characterization of Antibiotic-Loaded Nanocarriers for Dental Delivery |
title | Development and In Vitro Characterization of Antibiotic-Loaded Nanocarriers for Dental Delivery |
title_full | Development and In Vitro Characterization of Antibiotic-Loaded Nanocarriers for Dental Delivery |
title_fullStr | Development and In Vitro Characterization of Antibiotic-Loaded Nanocarriers for Dental Delivery |
title_full_unstemmed | Development and In Vitro Characterization of Antibiotic-Loaded Nanocarriers for Dental Delivery |
title_short | Development and In Vitro Characterization of Antibiotic-Loaded Nanocarriers for Dental Delivery |
title_sort | development and in vitro characterization of antibiotic-loaded nanocarriers for dental delivery |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096469/ https://www.ncbi.nlm.nih.gov/pubmed/37049683 http://dx.doi.org/10.3390/molecules28072914 |
work_keys_str_mv | AT ghazwanimohammed developmentandinvitrocharacterizationofantibioticloadednanocarriersfordentaldelivery AT vasudevanrajalakshimi developmentandinvitrocharacterizationofantibioticloadednanocarriersfordentaldelivery AT kandasamygeetha developmentandinvitrocharacterizationofantibioticloadednanocarriersfordentaldelivery AT haniumme developmentandinvitrocharacterizationofantibioticloadednanocarriersfordentaldelivery AT niharikagaddam developmentandinvitrocharacterizationofantibioticloadednanocarriersfordentaldelivery AT naredlamanusri developmentandinvitrocharacterizationofantibioticloadednanocarriersfordentaldelivery AT devanandanpraveen developmentandinvitrocharacterizationofantibioticloadednanocarriersfordentaldelivery AT puvvadaranadheerchowdary developmentandinvitrocharacterizationofantibioticloadednanocarriersfordentaldelivery AT almehiziaabdulrahmana developmentandinvitrocharacterizationofantibioticloadednanocarriersfordentaldelivery AT hakamiabdulrahimr developmentandinvitrocharacterizationofantibioticloadednanocarriersfordentaldelivery AT dhurkerajeshri developmentandinvitrocharacterizationofantibioticloadednanocarriersfordentaldelivery |