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Bioactive Materials Based on Hydroxypropyl Methylcellulose and Silver Nanoparticles: Structural-Morphological Characterization and Antimicrobial Testing

The progress achieved in recent years in the biomedical field justifies the objective evaluation of new techniques and materials obtained by using silver in different forms as metallic silver, silver salts, and nanoparticles. Thus, the antibacterial, antiviral, antifungal, antioxidant, and anti-infl...

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Autores principales: Filimon, Anca, Onofrei, Mihaela Dorina, Bargan, Alexandra, Stoica, Iuliana, Dunca, Simona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096613/
https://www.ncbi.nlm.nih.gov/pubmed/37050239
http://dx.doi.org/10.3390/polym15071625
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author Filimon, Anca
Onofrei, Mihaela Dorina
Bargan, Alexandra
Stoica, Iuliana
Dunca, Simona
author_facet Filimon, Anca
Onofrei, Mihaela Dorina
Bargan, Alexandra
Stoica, Iuliana
Dunca, Simona
author_sort Filimon, Anca
collection PubMed
description The progress achieved in recent years in the biomedical field justifies the objective evaluation of new techniques and materials obtained by using silver in different forms as metallic silver, silver salts, and nanoparticles. Thus, the antibacterial, antiviral, antifungal, antioxidant, and anti-inflammatory activity of silver nanoparticles (AgNPs) confers to newly obtained materials characteristics that make them ideal candidates in a wide spectrum of applications. In the present study, the use of hydroxypropyl methyl cellulose (HPMC) in the new formulation, by embedding AgNPs with antibacterial activity, using poly(N-vinylpyrrolidone) (PVP) as a stabilizing agent was investigated. AgNPs were incorporated in HPMC solutions, by thermal reduction of silver ions to silver nanoparticles, using PVP as a stabilizer; a technique that ensures the efficiency and selectivity of the obtained materials. The rheological properties, morphology, in vitro antimicrobial activity, and stability/catching of Ag nanoparticles in resulting HPMC/PVP-AgNPs materials were evaluated. The obtained rheological parameters highlight the multifunctional roles of PVP, focusing on the stabilizing effect of new formulations but also the optimization of some properties of the studied materials. The silver amount was quantified using the spectroscopy techniques (energy-dispersive X-ray fluorescence (XRF), energy-dispersive X-ray spectroscopy (EDX)), while formation of the AgNPs was confirmed using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Also, the morphological examination (Atomic Force Microscopy (AFM) and Scanning electron microscopy (SEM)) by means of the texture roughness parameters has evidenced favorable characteristics for targeted applications. Antibacterial activity was tested against Escherichia coli and Staphylococcus aureus and was found to be substantially improved was silver was added in the studied systems.
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spelling pubmed-100966132023-04-13 Bioactive Materials Based on Hydroxypropyl Methylcellulose and Silver Nanoparticles: Structural-Morphological Characterization and Antimicrobial Testing Filimon, Anca Onofrei, Mihaela Dorina Bargan, Alexandra Stoica, Iuliana Dunca, Simona Polymers (Basel) Article The progress achieved in recent years in the biomedical field justifies the objective evaluation of new techniques and materials obtained by using silver in different forms as metallic silver, silver salts, and nanoparticles. Thus, the antibacterial, antiviral, antifungal, antioxidant, and anti-inflammatory activity of silver nanoparticles (AgNPs) confers to newly obtained materials characteristics that make them ideal candidates in a wide spectrum of applications. In the present study, the use of hydroxypropyl methyl cellulose (HPMC) in the new formulation, by embedding AgNPs with antibacterial activity, using poly(N-vinylpyrrolidone) (PVP) as a stabilizing agent was investigated. AgNPs were incorporated in HPMC solutions, by thermal reduction of silver ions to silver nanoparticles, using PVP as a stabilizer; a technique that ensures the efficiency and selectivity of the obtained materials. The rheological properties, morphology, in vitro antimicrobial activity, and stability/catching of Ag nanoparticles in resulting HPMC/PVP-AgNPs materials were evaluated. The obtained rheological parameters highlight the multifunctional roles of PVP, focusing on the stabilizing effect of new formulations but also the optimization of some properties of the studied materials. The silver amount was quantified using the spectroscopy techniques (energy-dispersive X-ray fluorescence (XRF), energy-dispersive X-ray spectroscopy (EDX)), while formation of the AgNPs was confirmed using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Also, the morphological examination (Atomic Force Microscopy (AFM) and Scanning electron microscopy (SEM)) by means of the texture roughness parameters has evidenced favorable characteristics for targeted applications. Antibacterial activity was tested against Escherichia coli and Staphylococcus aureus and was found to be substantially improved was silver was added in the studied systems. MDPI 2023-03-24 /pmc/articles/PMC10096613/ /pubmed/37050239 http://dx.doi.org/10.3390/polym15071625 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Filimon, Anca
Onofrei, Mihaela Dorina
Bargan, Alexandra
Stoica, Iuliana
Dunca, Simona
Bioactive Materials Based on Hydroxypropyl Methylcellulose and Silver Nanoparticles: Structural-Morphological Characterization and Antimicrobial Testing
title Bioactive Materials Based on Hydroxypropyl Methylcellulose and Silver Nanoparticles: Structural-Morphological Characterization and Antimicrobial Testing
title_full Bioactive Materials Based on Hydroxypropyl Methylcellulose and Silver Nanoparticles: Structural-Morphological Characterization and Antimicrobial Testing
title_fullStr Bioactive Materials Based on Hydroxypropyl Methylcellulose and Silver Nanoparticles: Structural-Morphological Characterization and Antimicrobial Testing
title_full_unstemmed Bioactive Materials Based on Hydroxypropyl Methylcellulose and Silver Nanoparticles: Structural-Morphological Characterization and Antimicrobial Testing
title_short Bioactive Materials Based on Hydroxypropyl Methylcellulose and Silver Nanoparticles: Structural-Morphological Characterization and Antimicrobial Testing
title_sort bioactive materials based on hydroxypropyl methylcellulose and silver nanoparticles: structural-morphological characterization and antimicrobial testing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096613/
https://www.ncbi.nlm.nih.gov/pubmed/37050239
http://dx.doi.org/10.3390/polym15071625
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