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Dysbiosis of the Gut Microbiota and Kynurenine (Kyn) Pathway Activity as Potential Biomarkers in Patients with Major Depressive Disorder

With increasing attention paid to the concept of the microbiota–gut–brain axis, mounting evidence reveals that the gut microbiota is involved in a variety of neurological and psychiatric diseases. However, gut microbiota changes in major depressive disorder (MDD) patients and their association with...

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Autores principales: Lin, Ping, Li, Dan, Shi, Yun, Li, Qingtian, Guo, Xiaokui, Dong, Ke, Chen, Qing, Lou, Xiaoyan, Li, Zhenhua, Li, Ping, Jin, Weifeng, Chen, Shuzi, Sun, Yang, Sun, Jing, Cheng, Xunjia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096701/
https://www.ncbi.nlm.nih.gov/pubmed/37049591
http://dx.doi.org/10.3390/nu15071752
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author Lin, Ping
Li, Dan
Shi, Yun
Li, Qingtian
Guo, Xiaokui
Dong, Ke
Chen, Qing
Lou, Xiaoyan
Li, Zhenhua
Li, Ping
Jin, Weifeng
Chen, Shuzi
Sun, Yang
Sun, Jing
Cheng, Xunjia
author_facet Lin, Ping
Li, Dan
Shi, Yun
Li, Qingtian
Guo, Xiaokui
Dong, Ke
Chen, Qing
Lou, Xiaoyan
Li, Zhenhua
Li, Ping
Jin, Weifeng
Chen, Shuzi
Sun, Yang
Sun, Jing
Cheng, Xunjia
author_sort Lin, Ping
collection PubMed
description With increasing attention paid to the concept of the microbiota–gut–brain axis, mounting evidence reveals that the gut microbiota is involved in a variety of neurological and psychiatric diseases. However, gut microbiota changes in major depressive disorder (MDD) patients and their association with disease mechanisms remain undefined. Fifty MDD patients and sixty healthy controls were recruited from the Shanghai Healthy Mental Center, China. Fecal samples were collected, and the compositional characteristics of the intestinal flora were determined in MDD patients by MiSeq sequencing. Venous blood was collected for the detection of plasma indoleamine-2,3-dioxygenase (Ido), kynurenine (Kyn) and tryptophan (Trp) levels. Stool samples of bacterial 16S sequencing was carried out. A total of 2,705,809 optimized sequences were obtained, with an average of 54,116 per sample. More unique OTUs were observed at the family, genus and species levels in the control group compared with the MDD cases. Further analysis showed significant changes in the α- and β-diversities and relative abundance levels of gut microbial entities in MDD patients, as well as elevated amounts of Ido and Kyn indicating Kyn pathway activation, KEGG bacterial 16S function prediction analysis shows a variety of amino acids and metabolic (including Ido, Trp and Kyn) changes in the body of patients with MDD. These may result in increased neurotoxic metabolites and reduced generation of serotonin in the disease process. These changed factors may potentially be utilized as biomarkers for MDD in the future, playing more important roles in the disease course.
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spelling pubmed-100967012023-04-13 Dysbiosis of the Gut Microbiota and Kynurenine (Kyn) Pathway Activity as Potential Biomarkers in Patients with Major Depressive Disorder Lin, Ping Li, Dan Shi, Yun Li, Qingtian Guo, Xiaokui Dong, Ke Chen, Qing Lou, Xiaoyan Li, Zhenhua Li, Ping Jin, Weifeng Chen, Shuzi Sun, Yang Sun, Jing Cheng, Xunjia Nutrients Article With increasing attention paid to the concept of the microbiota–gut–brain axis, mounting evidence reveals that the gut microbiota is involved in a variety of neurological and psychiatric diseases. However, gut microbiota changes in major depressive disorder (MDD) patients and their association with disease mechanisms remain undefined. Fifty MDD patients and sixty healthy controls were recruited from the Shanghai Healthy Mental Center, China. Fecal samples were collected, and the compositional characteristics of the intestinal flora were determined in MDD patients by MiSeq sequencing. Venous blood was collected for the detection of plasma indoleamine-2,3-dioxygenase (Ido), kynurenine (Kyn) and tryptophan (Trp) levels. Stool samples of bacterial 16S sequencing was carried out. A total of 2,705,809 optimized sequences were obtained, with an average of 54,116 per sample. More unique OTUs were observed at the family, genus and species levels in the control group compared with the MDD cases. Further analysis showed significant changes in the α- and β-diversities and relative abundance levels of gut microbial entities in MDD patients, as well as elevated amounts of Ido and Kyn indicating Kyn pathway activation, KEGG bacterial 16S function prediction analysis shows a variety of amino acids and metabolic (including Ido, Trp and Kyn) changes in the body of patients with MDD. These may result in increased neurotoxic metabolites and reduced generation of serotonin in the disease process. These changed factors may potentially be utilized as biomarkers for MDD in the future, playing more important roles in the disease course. MDPI 2023-04-03 /pmc/articles/PMC10096701/ /pubmed/37049591 http://dx.doi.org/10.3390/nu15071752 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Ping
Li, Dan
Shi, Yun
Li, Qingtian
Guo, Xiaokui
Dong, Ke
Chen, Qing
Lou, Xiaoyan
Li, Zhenhua
Li, Ping
Jin, Weifeng
Chen, Shuzi
Sun, Yang
Sun, Jing
Cheng, Xunjia
Dysbiosis of the Gut Microbiota and Kynurenine (Kyn) Pathway Activity as Potential Biomarkers in Patients with Major Depressive Disorder
title Dysbiosis of the Gut Microbiota and Kynurenine (Kyn) Pathway Activity as Potential Biomarkers in Patients with Major Depressive Disorder
title_full Dysbiosis of the Gut Microbiota and Kynurenine (Kyn) Pathway Activity as Potential Biomarkers in Patients with Major Depressive Disorder
title_fullStr Dysbiosis of the Gut Microbiota and Kynurenine (Kyn) Pathway Activity as Potential Biomarkers in Patients with Major Depressive Disorder
title_full_unstemmed Dysbiosis of the Gut Microbiota and Kynurenine (Kyn) Pathway Activity as Potential Biomarkers in Patients with Major Depressive Disorder
title_short Dysbiosis of the Gut Microbiota and Kynurenine (Kyn) Pathway Activity as Potential Biomarkers in Patients with Major Depressive Disorder
title_sort dysbiosis of the gut microbiota and kynurenine (kyn) pathway activity as potential biomarkers in patients with major depressive disorder
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096701/
https://www.ncbi.nlm.nih.gov/pubmed/37049591
http://dx.doi.org/10.3390/nu15071752
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