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A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus

Cytoplasmic male sterility (CMS) is the main mechanism employed to utilize the heterosis of Brassica napus. CMS three-line rapeseed hybrids have dramatically enhanced yield and brought about the global revolution of hybrid varieties, replacing conventional crop varieties. Over the last half century,...

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Autores principales: Zhang, Yanfeng, An, Ran, Song, Min, Xie, Changgen, Wei, Shihao, Wang, Daojie, Dong, Yuhong, Jia, Qingli, Huang, Shuhua, Mu, Jianxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096742/
https://www.ncbi.nlm.nih.gov/pubmed/37050140
http://dx.doi.org/10.3390/plants12071514
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author Zhang, Yanfeng
An, Ran
Song, Min
Xie, Changgen
Wei, Shihao
Wang, Daojie
Dong, Yuhong
Jia, Qingli
Huang, Shuhua
Mu, Jianxin
author_facet Zhang, Yanfeng
An, Ran
Song, Min
Xie, Changgen
Wei, Shihao
Wang, Daojie
Dong, Yuhong
Jia, Qingli
Huang, Shuhua
Mu, Jianxin
author_sort Zhang, Yanfeng
collection PubMed
description Cytoplasmic male sterility (CMS) is the main mechanism employed to utilize the heterosis of Brassica napus. CMS three-line rapeseed hybrids have dramatically enhanced yield and brought about the global revolution of hybrid varieties, replacing conventional crop varieties. Over the last half century, China has led the development of hybrid Brassica napus varieties. Two sterile lines, polima (pol) and shaan 2A, were of particular importance for the establishment of three-line hybrid systems in rapeseed, which has opened up a new era of heterosis utilization. However, in current breeding practices, it takes up to three years to identify the restorer or maintainer relationship and the cytoplasmic type of any inbred material. This greatly affects the breeding speed of new varieties and inhibits the rapid development of the rapeseed industry. To address this problem, we developed a set of molecular markers for the identification of fertile cytoplasmic gene N and sterile cytoplasmic gene S, as well as for the fertile nucleus gene R and sterile nucleus gene r, based on differences in the gene sequences between the CMS line, maintainer line and restorer line of Brassica napus. Combining these markers can accurately identify the CMS line, maintainer and restorer of both the pol and shaan systems, as well as their hybrids. These markers can not only be used to identify of the maintainer and restorer relationship of inbred materials; they can also be used as general molecular markers to identify the CMS-type hybrid purity of pol and shaan systems.
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spelling pubmed-100967422023-04-13 A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus Zhang, Yanfeng An, Ran Song, Min Xie, Changgen Wei, Shihao Wang, Daojie Dong, Yuhong Jia, Qingli Huang, Shuhua Mu, Jianxin Plants (Basel) Article Cytoplasmic male sterility (CMS) is the main mechanism employed to utilize the heterosis of Brassica napus. CMS three-line rapeseed hybrids have dramatically enhanced yield and brought about the global revolution of hybrid varieties, replacing conventional crop varieties. Over the last half century, China has led the development of hybrid Brassica napus varieties. Two sterile lines, polima (pol) and shaan 2A, were of particular importance for the establishment of three-line hybrid systems in rapeseed, which has opened up a new era of heterosis utilization. However, in current breeding practices, it takes up to three years to identify the restorer or maintainer relationship and the cytoplasmic type of any inbred material. This greatly affects the breeding speed of new varieties and inhibits the rapid development of the rapeseed industry. To address this problem, we developed a set of molecular markers for the identification of fertile cytoplasmic gene N and sterile cytoplasmic gene S, as well as for the fertile nucleus gene R and sterile nucleus gene r, based on differences in the gene sequences between the CMS line, maintainer line and restorer line of Brassica napus. Combining these markers can accurately identify the CMS line, maintainer and restorer of both the pol and shaan systems, as well as their hybrids. These markers can not only be used to identify of the maintainer and restorer relationship of inbred materials; they can also be used as general molecular markers to identify the CMS-type hybrid purity of pol and shaan systems. MDPI 2023-03-30 /pmc/articles/PMC10096742/ /pubmed/37050140 http://dx.doi.org/10.3390/plants12071514 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Yanfeng
An, Ran
Song, Min
Xie, Changgen
Wei, Shihao
Wang, Daojie
Dong, Yuhong
Jia, Qingli
Huang, Shuhua
Mu, Jianxin
A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus
title A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus
title_full A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus
title_fullStr A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus
title_full_unstemmed A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus
title_short A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus
title_sort set of molecular markers to accelerate breeding and determine seed purity of cms three-line hybrids in brassica napus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096742/
https://www.ncbi.nlm.nih.gov/pubmed/37050140
http://dx.doi.org/10.3390/plants12071514
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