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Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells

Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particula...

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Autores principales: Alsrhani, Abdullah, Elderdery, Abozer Y., Alzahrani, Badr, Alzerwi, Nasser A. N., Althobiti, Maryam Musleh, Rayzah, Musaed, Idrees, Bandar, Elkhalifa, Ahmed M. E., Subbiah, Suresh K., Mok, Pooi Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096807/
https://www.ncbi.nlm.nih.gov/pubmed/37049991
http://dx.doi.org/10.3390/molecules28073228
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author Alsrhani, Abdullah
Elderdery, Abozer Y.
Alzahrani, Badr
Alzerwi, Nasser A. N.
Althobiti, Maryam Musleh
Rayzah, Musaed
Idrees, Bandar
Elkhalifa, Ahmed M. E.
Subbiah, Suresh K.
Mok, Pooi Ling
author_facet Alsrhani, Abdullah
Elderdery, Abozer Y.
Alzahrani, Badr
Alzerwi, Nasser A. N.
Althobiti, Maryam Musleh
Rayzah, Musaed
Idrees, Bandar
Elkhalifa, Ahmed M. E.
Subbiah, Suresh K.
Mok, Pooi Ling
author_sort Alsrhani, Abdullah
collection PubMed
description Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particularly for cancer therapy. Specifically, albumin-based nanoparticles are a developing platform for the administration of diverse chemotherapy drugs owing to their biocompatibility and non-toxicity. Visnagin, a naturally derived furanochromone, treats cancers, epilepsy, angina, coughs, and inflammatory illnesses. In the current study, the synthesis and characterization of albumin visnagin (AV) nanoparticles (NPs) using a variety of techniques such as transmission electron microscopy, UV-visible, Fourier transform infrared, energy dispersive X-ray composition analysis, field emission scanning electron microscopy, photoluminescence, X-Ray diffraction, and dynamic light scattering analyses have been carried out. The MTT test, dual AO/EB, DCFH-DA, Annexin-V-FITC/PI, Propidium iodide staining techniques as well as analysis of apoptotic proteins, antioxidant enzymes, and PI3K/Akt/mTOR signaling analysis was performed to examine the NPs’ efficacy to suppress MDA-MB-468 cell lines. The NPs decreased cell viability increased the amount of ROS in the cells, disrupted membrane integrity, decreased the level of antioxidant enzymes, induced cell cycle arrest, and activated the PI3K/Akt/mTOR signaling cascade, ultimately leading to cell death. Thus, AV NPs possesses huge potential to be employed as a strong anticancer therapy alternative.
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spelling pubmed-100968072023-04-13 Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells Alsrhani, Abdullah Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling Molecules Article Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particularly for cancer therapy. Specifically, albumin-based nanoparticles are a developing platform for the administration of diverse chemotherapy drugs owing to their biocompatibility and non-toxicity. Visnagin, a naturally derived furanochromone, treats cancers, epilepsy, angina, coughs, and inflammatory illnesses. In the current study, the synthesis and characterization of albumin visnagin (AV) nanoparticles (NPs) using a variety of techniques such as transmission electron microscopy, UV-visible, Fourier transform infrared, energy dispersive X-ray composition analysis, field emission scanning electron microscopy, photoluminescence, X-Ray diffraction, and dynamic light scattering analyses have been carried out. The MTT test, dual AO/EB, DCFH-DA, Annexin-V-FITC/PI, Propidium iodide staining techniques as well as analysis of apoptotic proteins, antioxidant enzymes, and PI3K/Akt/mTOR signaling analysis was performed to examine the NPs’ efficacy to suppress MDA-MB-468 cell lines. The NPs decreased cell viability increased the amount of ROS in the cells, disrupted membrane integrity, decreased the level of antioxidant enzymes, induced cell cycle arrest, and activated the PI3K/Akt/mTOR signaling cascade, ultimately leading to cell death. Thus, AV NPs possesses huge potential to be employed as a strong anticancer therapy alternative. MDPI 2023-04-04 /pmc/articles/PMC10096807/ /pubmed/37049991 http://dx.doi.org/10.3390/molecules28073228 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alsrhani, Abdullah
Elderdery, Abozer Y.
Alzahrani, Badr
Alzerwi, Nasser A. N.
Althobiti, Maryam Musleh
Rayzah, Musaed
Idrees, Bandar
Elkhalifa, Ahmed M. E.
Subbiah, Suresh K.
Mok, Pooi Ling
Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells
title Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells
title_full Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells
title_fullStr Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells
title_full_unstemmed Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells
title_short Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells
title_sort therapeutic potential of albumin nanoparticles encapsulated visnagin in mda-mb-468 triple-negative breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096807/
https://www.ncbi.nlm.nih.gov/pubmed/37049991
http://dx.doi.org/10.3390/molecules28073228
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