Cargando…
Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells
Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particula...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096807/ https://www.ncbi.nlm.nih.gov/pubmed/37049991 http://dx.doi.org/10.3390/molecules28073228 |
_version_ | 1785024426628087808 |
---|---|
author | Alsrhani, Abdullah Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling |
author_facet | Alsrhani, Abdullah Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling |
author_sort | Alsrhani, Abdullah |
collection | PubMed |
description | Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particularly for cancer therapy. Specifically, albumin-based nanoparticles are a developing platform for the administration of diverse chemotherapy drugs owing to their biocompatibility and non-toxicity. Visnagin, a naturally derived furanochromone, treats cancers, epilepsy, angina, coughs, and inflammatory illnesses. In the current study, the synthesis and characterization of albumin visnagin (AV) nanoparticles (NPs) using a variety of techniques such as transmission electron microscopy, UV-visible, Fourier transform infrared, energy dispersive X-ray composition analysis, field emission scanning electron microscopy, photoluminescence, X-Ray diffraction, and dynamic light scattering analyses have been carried out. The MTT test, dual AO/EB, DCFH-DA, Annexin-V-FITC/PI, Propidium iodide staining techniques as well as analysis of apoptotic proteins, antioxidant enzymes, and PI3K/Akt/mTOR signaling analysis was performed to examine the NPs’ efficacy to suppress MDA-MB-468 cell lines. The NPs decreased cell viability increased the amount of ROS in the cells, disrupted membrane integrity, decreased the level of antioxidant enzymes, induced cell cycle arrest, and activated the PI3K/Akt/mTOR signaling cascade, ultimately leading to cell death. Thus, AV NPs possesses huge potential to be employed as a strong anticancer therapy alternative. |
format | Online Article Text |
id | pubmed-10096807 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100968072023-04-13 Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells Alsrhani, Abdullah Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling Molecules Article Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particularly for cancer therapy. Specifically, albumin-based nanoparticles are a developing platform for the administration of diverse chemotherapy drugs owing to their biocompatibility and non-toxicity. Visnagin, a naturally derived furanochromone, treats cancers, epilepsy, angina, coughs, and inflammatory illnesses. In the current study, the synthesis and characterization of albumin visnagin (AV) nanoparticles (NPs) using a variety of techniques such as transmission electron microscopy, UV-visible, Fourier transform infrared, energy dispersive X-ray composition analysis, field emission scanning electron microscopy, photoluminescence, X-Ray diffraction, and dynamic light scattering analyses have been carried out. The MTT test, dual AO/EB, DCFH-DA, Annexin-V-FITC/PI, Propidium iodide staining techniques as well as analysis of apoptotic proteins, antioxidant enzymes, and PI3K/Akt/mTOR signaling analysis was performed to examine the NPs’ efficacy to suppress MDA-MB-468 cell lines. The NPs decreased cell viability increased the amount of ROS in the cells, disrupted membrane integrity, decreased the level of antioxidant enzymes, induced cell cycle arrest, and activated the PI3K/Akt/mTOR signaling cascade, ultimately leading to cell death. Thus, AV NPs possesses huge potential to be employed as a strong anticancer therapy alternative. MDPI 2023-04-04 /pmc/articles/PMC10096807/ /pubmed/37049991 http://dx.doi.org/10.3390/molecules28073228 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alsrhani, Abdullah Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells |
title | Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells |
title_full | Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells |
title_fullStr | Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells |
title_full_unstemmed | Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells |
title_short | Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells |
title_sort | therapeutic potential of albumin nanoparticles encapsulated visnagin in mda-mb-468 triple-negative breast cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096807/ https://www.ncbi.nlm.nih.gov/pubmed/37049991 http://dx.doi.org/10.3390/molecules28073228 |
work_keys_str_mv | AT alsrhaniabdullah therapeuticpotentialofalbuminnanoparticlesencapsulatedvisnagininmdamb468triplenegativebreastcancercells AT elderderyabozery therapeuticpotentialofalbuminnanoparticlesencapsulatedvisnagininmdamb468triplenegativebreastcancercells AT alzahranibadr therapeuticpotentialofalbuminnanoparticlesencapsulatedvisnagininmdamb468triplenegativebreastcancercells AT alzerwinasseran therapeuticpotentialofalbuminnanoparticlesencapsulatedvisnagininmdamb468triplenegativebreastcancercells AT althobitimaryammusleh therapeuticpotentialofalbuminnanoparticlesencapsulatedvisnagininmdamb468triplenegativebreastcancercells AT rayzahmusaed therapeuticpotentialofalbuminnanoparticlesencapsulatedvisnagininmdamb468triplenegativebreastcancercells AT idreesbandar therapeuticpotentialofalbuminnanoparticlesencapsulatedvisnagininmdamb468triplenegativebreastcancercells AT elkhalifaahmedme therapeuticpotentialofalbuminnanoparticlesencapsulatedvisnagininmdamb468triplenegativebreastcancercells AT subbiahsureshk therapeuticpotentialofalbuminnanoparticlesencapsulatedvisnagininmdamb468triplenegativebreastcancercells AT mokpooiling therapeuticpotentialofalbuminnanoparticlesencapsulatedvisnagininmdamb468triplenegativebreastcancercells |