Cargando…

Characterization of Delonix regia Flowers’ Pigment and Polysaccharides: Evaluating Their Antibacterial, Anticancer, and Antioxidant Activities and Their Application as a Natural Colorant and Sweetener in Beverages

In the present study, an attempt was made to investigate the in vitro antioxidant, anticancer, and antibacterial activities of Delonix regia, then in vivo evaluate its safety as a natural colorant and sweetener in beverages compared to synthetic colorant and sweetener in rats, then serve the beverag...

Descripción completa

Detalles Bibliográficos
Autores principales: Ebada, Doaa, Hefnawy, Hefnawy T., Gomaa, Ayman, Alghamdi, Amira M., Alharbi, Asmaa Ali, Almuhayawi, Mohammed S., Alharbi, Mohanned Talal, Awad, Ahmed, Al Jaouni, Soad K., Selim, Samy, Eldeeb, Gehad S., Namir, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096959/
https://www.ncbi.nlm.nih.gov/pubmed/37050006
http://dx.doi.org/10.3390/molecules28073243
_version_ 1785024464454418432
author Ebada, Doaa
Hefnawy, Hefnawy T.
Gomaa, Ayman
Alghamdi, Amira M.
Alharbi, Asmaa Ali
Almuhayawi, Mohammed S.
Alharbi, Mohanned Talal
Awad, Ahmed
Al Jaouni, Soad K.
Selim, Samy
Eldeeb, Gehad S.
Namir, Mohammad
author_facet Ebada, Doaa
Hefnawy, Hefnawy T.
Gomaa, Ayman
Alghamdi, Amira M.
Alharbi, Asmaa Ali
Almuhayawi, Mohammed S.
Alharbi, Mohanned Talal
Awad, Ahmed
Al Jaouni, Soad K.
Selim, Samy
Eldeeb, Gehad S.
Namir, Mohammad
author_sort Ebada, Doaa
collection PubMed
description In the present study, an attempt was made to investigate the in vitro antioxidant, anticancer, and antibacterial activities of Delonix regia, then in vivo evaluate its safety as a natural colorant and sweetener in beverages compared to synthetic colorant and sweetener in rats, then serve the beverages for sensory evaluation. Delonix regia flowers had high protein, polysaccharide, Ca, Na, Mg, K, and Fe contents. The Delonix regia pigment extract (DRPE) polysaccharides were separated and purified by gel permeation chromatography on Sephacryl S-200, characterized by rich polysaccharides (13.6 g/L). The HPLC sugar profile detected the monosaccharides in the extracted polysaccharides, composed of mannose, galactose, glucose, arabinose, and gluconic acid, and the structure of saccharides was confirmed by FTIR, which showed three active groups: carbonyl, hydrocarbon, and hydroxyl. On the other hand, the red pigment constituents of DRPE were detected by HPLC; the main compounds were delphinidin and cyanidin at 15 µg/mL. The DRPE contained a considerable amount (26.33 mg/g) of anthocyanins, phenolic compounds (64.7 mg/g), and flavonoids (10.30 mg/g), thus influencing the antioxidant activity of the DRPE, which scavenged 92% of DPPH free radicals. Additionally, it inhibited the population of pathogenic bacteria, including Staphylococcus aureus, Listeria monocyogenes, Salmonella typhimurum, and Pseudomonas aeruginosa, in the range of 30–90 μg/mL, in addition to inhibiting 85% of pancreatic cancer cell lines. On the in vivo level, the rats that were delivered a diet containing DRPE showed regular liver markers (AST, ALP, and ALT); kidney markers (urea and creatinine); high TP, TA, and GSH; and low MDA, while rats treated with synthetic dye and aspartame showed higher liver and kidney markers; lowered TP, TA, and GSH; and high MDA. After proving the safety of DRPE, it can be safely added to strawberry beverages. Significant sensorial traits, enhanced red color, and taste characterize the strawberry beverages supplemented with DRPE. The lightness and redness of strawberries were enhanced, and the color change ΔE values in DRPE-supplemented beverages ranged from 1.1 to 1.35 compared to 1.69 in controls, indicating the preservative role of DRPE on color. So, including DRPE in food formulation as a natural colorant and sweetener is recommended for preserving health and the environment.
format Online
Article
Text
id pubmed-10096959
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100969592023-04-13 Characterization of Delonix regia Flowers’ Pigment and Polysaccharides: Evaluating Their Antibacterial, Anticancer, and Antioxidant Activities and Their Application as a Natural Colorant and Sweetener in Beverages Ebada, Doaa Hefnawy, Hefnawy T. Gomaa, Ayman Alghamdi, Amira M. Alharbi, Asmaa Ali Almuhayawi, Mohammed S. Alharbi, Mohanned Talal Awad, Ahmed Al Jaouni, Soad K. Selim, Samy Eldeeb, Gehad S. Namir, Mohammad Molecules Article In the present study, an attempt was made to investigate the in vitro antioxidant, anticancer, and antibacterial activities of Delonix regia, then in vivo evaluate its safety as a natural colorant and sweetener in beverages compared to synthetic colorant and sweetener in rats, then serve the beverages for sensory evaluation. Delonix regia flowers had high protein, polysaccharide, Ca, Na, Mg, K, and Fe contents. The Delonix regia pigment extract (DRPE) polysaccharides were separated and purified by gel permeation chromatography on Sephacryl S-200, characterized by rich polysaccharides (13.6 g/L). The HPLC sugar profile detected the monosaccharides in the extracted polysaccharides, composed of mannose, galactose, glucose, arabinose, and gluconic acid, and the structure of saccharides was confirmed by FTIR, which showed three active groups: carbonyl, hydrocarbon, and hydroxyl. On the other hand, the red pigment constituents of DRPE were detected by HPLC; the main compounds were delphinidin and cyanidin at 15 µg/mL. The DRPE contained a considerable amount (26.33 mg/g) of anthocyanins, phenolic compounds (64.7 mg/g), and flavonoids (10.30 mg/g), thus influencing the antioxidant activity of the DRPE, which scavenged 92% of DPPH free radicals. Additionally, it inhibited the population of pathogenic bacteria, including Staphylococcus aureus, Listeria monocyogenes, Salmonella typhimurum, and Pseudomonas aeruginosa, in the range of 30–90 μg/mL, in addition to inhibiting 85% of pancreatic cancer cell lines. On the in vivo level, the rats that were delivered a diet containing DRPE showed regular liver markers (AST, ALP, and ALT); kidney markers (urea and creatinine); high TP, TA, and GSH; and low MDA, while rats treated with synthetic dye and aspartame showed higher liver and kidney markers; lowered TP, TA, and GSH; and high MDA. After proving the safety of DRPE, it can be safely added to strawberry beverages. Significant sensorial traits, enhanced red color, and taste characterize the strawberry beverages supplemented with DRPE. The lightness and redness of strawberries were enhanced, and the color change ΔE values in DRPE-supplemented beverages ranged from 1.1 to 1.35 compared to 1.69 in controls, indicating the preservative role of DRPE on color. So, including DRPE in food formulation as a natural colorant and sweetener is recommended for preserving health and the environment. MDPI 2023-04-05 /pmc/articles/PMC10096959/ /pubmed/37050006 http://dx.doi.org/10.3390/molecules28073243 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ebada, Doaa
Hefnawy, Hefnawy T.
Gomaa, Ayman
Alghamdi, Amira M.
Alharbi, Asmaa Ali
Almuhayawi, Mohammed S.
Alharbi, Mohanned Talal
Awad, Ahmed
Al Jaouni, Soad K.
Selim, Samy
Eldeeb, Gehad S.
Namir, Mohammad
Characterization of Delonix regia Flowers’ Pigment and Polysaccharides: Evaluating Their Antibacterial, Anticancer, and Antioxidant Activities and Their Application as a Natural Colorant and Sweetener in Beverages
title Characterization of Delonix regia Flowers’ Pigment and Polysaccharides: Evaluating Their Antibacterial, Anticancer, and Antioxidant Activities and Their Application as a Natural Colorant and Sweetener in Beverages
title_full Characterization of Delonix regia Flowers’ Pigment and Polysaccharides: Evaluating Their Antibacterial, Anticancer, and Antioxidant Activities and Their Application as a Natural Colorant and Sweetener in Beverages
title_fullStr Characterization of Delonix regia Flowers’ Pigment and Polysaccharides: Evaluating Their Antibacterial, Anticancer, and Antioxidant Activities and Their Application as a Natural Colorant and Sweetener in Beverages
title_full_unstemmed Characterization of Delonix regia Flowers’ Pigment and Polysaccharides: Evaluating Their Antibacterial, Anticancer, and Antioxidant Activities and Their Application as a Natural Colorant and Sweetener in Beverages
title_short Characterization of Delonix regia Flowers’ Pigment and Polysaccharides: Evaluating Their Antibacterial, Anticancer, and Antioxidant Activities and Their Application as a Natural Colorant and Sweetener in Beverages
title_sort characterization of delonix regia flowers’ pigment and polysaccharides: evaluating their antibacterial, anticancer, and antioxidant activities and their application as a natural colorant and sweetener in beverages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096959/
https://www.ncbi.nlm.nih.gov/pubmed/37050006
http://dx.doi.org/10.3390/molecules28073243
work_keys_str_mv AT ebadadoaa characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages
AT hefnawyhefnawyt characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages
AT gomaaayman characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages
AT alghamdiamiram characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages
AT alharbiasmaaali characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages
AT almuhayawimohammeds characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages
AT alharbimohannedtalal characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages
AT awadahmed characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages
AT aljaounisoadk characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages
AT selimsamy characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages
AT eldeebgehads characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages
AT namirmohammad characterizationofdelonixregiaflowerspigmentandpolysaccharidesevaluatingtheirantibacterialanticancerandantioxidantactivitiesandtheirapplicationasanaturalcolorantandsweetenerinbeverages