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HnRNPA2B1 Aggravates Inflammation by Promoting M1 Macrophage Polarization
Macrophages have critical contributions to both acute and chronic inflammatory diseases, for example, bowel disease and obesity, respectively. However, little is known about the post-transcriptional regulatory mechanisms in macrophage-mediated inflammatory diseases. hnRNPA2B1 (A2B1) is an RNA bindin...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096984/ https://www.ncbi.nlm.nih.gov/pubmed/37049395 http://dx.doi.org/10.3390/nu15071555 |
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author | Meng, Meiyao Cao, Yuxiang Zhang, Yankang Liu, Shuang Zhong, Yinzhao Wang, Dongmei Li, Dali Xu, Lingyan Ma, Xinran |
author_facet | Meng, Meiyao Cao, Yuxiang Zhang, Yankang Liu, Shuang Zhong, Yinzhao Wang, Dongmei Li, Dali Xu, Lingyan Ma, Xinran |
author_sort | Meng, Meiyao |
collection | PubMed |
description | Macrophages have critical contributions to both acute and chronic inflammatory diseases, for example, bowel disease and obesity, respectively. However, little is known about the post-transcriptional regulatory mechanisms in macrophage-mediated inflammatory diseases. hnRNPA2B1 (A2B1) is an RNA binding protein for mRNA fate determination. We showed that hnRNPA2B1 mRNA levels were increased in colon in dextran sodium sulfate (DSS)-induced colitis mice and in epididymal white adipose tissue (eWAT) and spleen of high-fat-diet (HFD)-induced obese mice. Consistently, mice with haploinsufficiency of A2B1 (A2B1 HET) are protected against DSS-induced acute colitis and HFD-induced obesity, with decreased M1 macrophages polarization in colon, eWAT and spleen. Mechanistically, A2B1 mRNA and protein levels were increased in LPS-stimulated RAW 264.7 macrophages, and A2B1 enhanced RNA stability of pro-inflammatory genes Tnfα, Il-6 and Il-1β for the regulation of macrophages polarization. Interestingly, A2B1 HET mice exhibited reduced white fat expansion, which was influenced by macrophages, since conditioned medium from macrophages with A2B1 manipulation significantly changed preadipocyte proliferation. Our data demonstrate that A2B1 plays a vital role in macrophage-mediated inflammation via regulating mRNA stability, suggesting that A2B1 may be served as a promising target for the intervention of acute and chronic inflammatory diseases. |
format | Online Article Text |
id | pubmed-10096984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100969842023-04-13 HnRNPA2B1 Aggravates Inflammation by Promoting M1 Macrophage Polarization Meng, Meiyao Cao, Yuxiang Zhang, Yankang Liu, Shuang Zhong, Yinzhao Wang, Dongmei Li, Dali Xu, Lingyan Ma, Xinran Nutrients Article Macrophages have critical contributions to both acute and chronic inflammatory diseases, for example, bowel disease and obesity, respectively. However, little is known about the post-transcriptional regulatory mechanisms in macrophage-mediated inflammatory diseases. hnRNPA2B1 (A2B1) is an RNA binding protein for mRNA fate determination. We showed that hnRNPA2B1 mRNA levels were increased in colon in dextran sodium sulfate (DSS)-induced colitis mice and in epididymal white adipose tissue (eWAT) and spleen of high-fat-diet (HFD)-induced obese mice. Consistently, mice with haploinsufficiency of A2B1 (A2B1 HET) are protected against DSS-induced acute colitis and HFD-induced obesity, with decreased M1 macrophages polarization in colon, eWAT and spleen. Mechanistically, A2B1 mRNA and protein levels were increased in LPS-stimulated RAW 264.7 macrophages, and A2B1 enhanced RNA stability of pro-inflammatory genes Tnfα, Il-6 and Il-1β for the regulation of macrophages polarization. Interestingly, A2B1 HET mice exhibited reduced white fat expansion, which was influenced by macrophages, since conditioned medium from macrophages with A2B1 manipulation significantly changed preadipocyte proliferation. Our data demonstrate that A2B1 plays a vital role in macrophage-mediated inflammation via regulating mRNA stability, suggesting that A2B1 may be served as a promising target for the intervention of acute and chronic inflammatory diseases. MDPI 2023-03-23 /pmc/articles/PMC10096984/ /pubmed/37049395 http://dx.doi.org/10.3390/nu15071555 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Meng, Meiyao Cao, Yuxiang Zhang, Yankang Liu, Shuang Zhong, Yinzhao Wang, Dongmei Li, Dali Xu, Lingyan Ma, Xinran HnRNPA2B1 Aggravates Inflammation by Promoting M1 Macrophage Polarization |
title | HnRNPA2B1 Aggravates Inflammation by Promoting M1 Macrophage Polarization |
title_full | HnRNPA2B1 Aggravates Inflammation by Promoting M1 Macrophage Polarization |
title_fullStr | HnRNPA2B1 Aggravates Inflammation by Promoting M1 Macrophage Polarization |
title_full_unstemmed | HnRNPA2B1 Aggravates Inflammation by Promoting M1 Macrophage Polarization |
title_short | HnRNPA2B1 Aggravates Inflammation by Promoting M1 Macrophage Polarization |
title_sort | hnrnpa2b1 aggravates inflammation by promoting m1 macrophage polarization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096984/ https://www.ncbi.nlm.nih.gov/pubmed/37049395 http://dx.doi.org/10.3390/nu15071555 |
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