Cargando…

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient mic...

Descripción completa

Detalles Bibliográficos
Autores principales: Gianguzzi, Valeria, Barone, Giulio, Di Gristina, Emilio, Sottile, Francesco, Domina, Gianniantonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097119/
https://www.ncbi.nlm.nih.gov/pubmed/37050156
http://dx.doi.org/10.3390/plants12071530
_version_ 1785024503135338496
author Gianguzzi, Valeria
Barone, Giulio
Di Gristina, Emilio
Sottile, Francesco
Domina, Gianniantonio
author_facet Gianguzzi, Valeria
Barone, Giulio
Di Gristina, Emilio
Sottile, Francesco
Domina, Gianniantonio
author_sort Gianguzzi, Valeria
collection PubMed
description The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for Adenostyles by evaluating the effect of different plant growth regulators on leaf explants. Adenostyles alpina subsp. macrocephala (Asterace) is a perennial herbaceous plant endemic to Calabria (Southern Italy). The genus Adenostyles includes three species confined to the mountains of the Mediterranean and southern Europe. For callus induction, media supplemented with different concentrations of Benzylaminopurine (BAP) (0.5, 1, 2, and 3 mg L(−1)), Naphthaleneacetic Acid (NAA) (1 mg L(−1)), and 2,4-Dichlorophenoxyacetic Acid (2,4-D) (1 mg L(−1)) were tested. Shoot regeneration and proliferation were obtained in media supplemented with BAP (1, 2, and 3 mg L(−1)) and NAA (1 mg L(−1)). Root induction was obtained in media supplemented with IBA (0.25, 0.50, and 1 mg L(−1)) and NAA (0.25, 0.50, and 1 mg L(−1)). Statistically significant differences in callus induction and shoot regeneration were observed between the various media tested. The medium containing Murashige and Skoog (MS) supplemented with 3 mg L(−1) of BAP and 1 mg L(−1) of NAA showed the highest percentage of callus induction and increased shoot regeneration. The regenerated shoots showed more effective root induction in the hormone-free MS medium and in the presence of Indole-3-Butyric Acid (IBA) at concentrations of 0.25, 0.50, and 1 mg L(−1). These results can be used as a basis for the preparation of a micropropagation protocol for different taxa of Adenostyles, as well as other species of Asteraceae specialized to the Mediterranean mountain habitat.
format Online
Article
Text
id pubmed-10097119
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100971192023-04-13 Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study Gianguzzi, Valeria Barone, Giulio Di Gristina, Emilio Sottile, Francesco Domina, Gianniantonio Plants (Basel) Article The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for Adenostyles by evaluating the effect of different plant growth regulators on leaf explants. Adenostyles alpina subsp. macrocephala (Asterace) is a perennial herbaceous plant endemic to Calabria (Southern Italy). The genus Adenostyles includes three species confined to the mountains of the Mediterranean and southern Europe. For callus induction, media supplemented with different concentrations of Benzylaminopurine (BAP) (0.5, 1, 2, and 3 mg L(−1)), Naphthaleneacetic Acid (NAA) (1 mg L(−1)), and 2,4-Dichlorophenoxyacetic Acid (2,4-D) (1 mg L(−1)) were tested. Shoot regeneration and proliferation were obtained in media supplemented with BAP (1, 2, and 3 mg L(−1)) and NAA (1 mg L(−1)). Root induction was obtained in media supplemented with IBA (0.25, 0.50, and 1 mg L(−1)) and NAA (0.25, 0.50, and 1 mg L(−1)). Statistically significant differences in callus induction and shoot regeneration were observed between the various media tested. The medium containing Murashige and Skoog (MS) supplemented with 3 mg L(−1) of BAP and 1 mg L(−1) of NAA showed the highest percentage of callus induction and increased shoot regeneration. The regenerated shoots showed more effective root induction in the hormone-free MS medium and in the presence of Indole-3-Butyric Acid (IBA) at concentrations of 0.25, 0.50, and 1 mg L(−1). These results can be used as a basis for the preparation of a micropropagation protocol for different taxa of Adenostyles, as well as other species of Asteraceae specialized to the Mediterranean mountain habitat. MDPI 2023-04-01 /pmc/articles/PMC10097119/ /pubmed/37050156 http://dx.doi.org/10.3390/plants12071530 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gianguzzi, Valeria
Barone, Giulio
Di Gristina, Emilio
Sottile, Francesco
Domina, Gianniantonio
Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study
title Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study
title_full Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study
title_fullStr Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study
title_full_unstemmed Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study
title_short Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study
title_sort micropropagation of endemic endangered taxa of the italian flora: adenostyles alpina subsp. macrocephala (asteraceae), as a case study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097119/
https://www.ncbi.nlm.nih.gov/pubmed/37050156
http://dx.doi.org/10.3390/plants12071530
work_keys_str_mv AT gianguzzivaleria micropropagationofendemicendangeredtaxaoftheitalianfloraadenostylesalpinasubspmacrocephalaasteraceaeasacasestudy
AT baronegiulio micropropagationofendemicendangeredtaxaoftheitalianfloraadenostylesalpinasubspmacrocephalaasteraceaeasacasestudy
AT digristinaemilio micropropagationofendemicendangeredtaxaoftheitalianfloraadenostylesalpinasubspmacrocephalaasteraceaeasacasestudy
AT sottilefrancesco micropropagationofendemicendangeredtaxaoftheitalianfloraadenostylesalpinasubspmacrocephalaasteraceaeasacasestudy
AT dominagianniantonio micropropagationofendemicendangeredtaxaoftheitalianfloraadenostylesalpinasubspmacrocephalaasteraceaeasacasestudy