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Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study

Objective: The present study aimed to evaluate the effects of enriched hen egg consumption on endothelium-dependent vasodilation (EDV) and the role of cyclooxygenases in EDV in the microcirculation of young healthy individuals. This study hypothesizes that Nutri4 eggs will improve endothelial functi...

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Autores principales: Šušnjara, Petar, Mihaljević, Zrinka, Stupin, Ana, Kolobarić, Nikolina, Matić, Anita, Jukić, Ivana, Kralik, Zlata, Kralik, Gordana, Miloloža, Anđelina, Pavošević, Tihana, Šerić, Vatroslav, Lončarić, Zdenko, Kerovec, Darko, Galović, Olivera, Drenjančević, Ines
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097130/
https://www.ncbi.nlm.nih.gov/pubmed/37049437
http://dx.doi.org/10.3390/nu15071599
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author Šušnjara, Petar
Mihaljević, Zrinka
Stupin, Ana
Kolobarić, Nikolina
Matić, Anita
Jukić, Ivana
Kralik, Zlata
Kralik, Gordana
Miloloža, Anđelina
Pavošević, Tihana
Šerić, Vatroslav
Lončarić, Zdenko
Kerovec, Darko
Galović, Olivera
Drenjančević, Ines
author_facet Šušnjara, Petar
Mihaljević, Zrinka
Stupin, Ana
Kolobarić, Nikolina
Matić, Anita
Jukić, Ivana
Kralik, Zlata
Kralik, Gordana
Miloloža, Anđelina
Pavošević, Tihana
Šerić, Vatroslav
Lončarić, Zdenko
Kerovec, Darko
Galović, Olivera
Drenjančević, Ines
author_sort Šušnjara, Petar
collection PubMed
description Objective: The present study aimed to evaluate the effects of enriched hen egg consumption on endothelium-dependent vasodilation (EDV) and the role of cyclooxygenases in EDV in the microcirculation of young healthy individuals. This study hypothesizes that Nutri4 eggs will improve endothelial function, which will be manifested by changes in microcirculatory flow measured by a laser Doppler flowmeter (LDF) during reactive hyperemia in response to vascular occlusion, in which n-3 PUFA plays an important role as well as its degradation pathway by cyclooxygenases. Materials and Methods: Participants consumed three eggs per day for three weeks: The control group (CTRL, n = 14) consumed regular hen eggs (approximately 0.330 mg of lutein, 1.785 mg of vitamin E, 0.054 mg of selenium and 438 mg of n-3 PUFAs daily) and Nutri4 group (n = 20) consumed enriched eggs (approximately 1.85 mg of lutein, 0.06 mg of selenium, 3.29 mg of vitamin E, and 1026 mg of n-3 PUFAs daily). Skin microvascular blood flow in response to EDV (post-occlusive reactive hyperemia (PORH) and iontophoresis of acetylcholine (AChID)) and sodium nitroprusside (SNPID; endothelium-independent) was assessed by laser Doppler flowmetry before and after dietary protocol and in a separate group of participants who were administered perorally 100 mg of indomethacin before microvascular response assessment. Arterial blood pressure, heart rate, serum lipid, and liver enzymes, anthropometric measurements, protein expression of cyclooxygenase 1 (COX-1), cyclooxygenase 2 (COX-2), neuronal nitric oxide synthases (nNOS), inducible nitric oxide synthases (iNOS), and endothelial nitric oxide synthases (eNOS) were measured before and after dietary protocol. Results: PORH and AChID were significantly enhanced, and SNPID remained unchanged in the Nutri4 group, while none was changed in the CTRL following a respective diet. PORH decreased after administration of indomethacin in Nutri4 after dietary protocol. Protein expression of COX-2 was significantly higher in the Nutri4 group compared to the CTRL after the dietary protocol. Conclusion: Consumption of enriched eggs improves microvascular EDV in healthy young subjects. Results suggest an element of n-3 PUFAs metabolites via the cyclooxygenases pathway in enhanced reactive hyperemia.
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spelling pubmed-100971302023-04-13 Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study Šušnjara, Petar Mihaljević, Zrinka Stupin, Ana Kolobarić, Nikolina Matić, Anita Jukić, Ivana Kralik, Zlata Kralik, Gordana Miloloža, Anđelina Pavošević, Tihana Šerić, Vatroslav Lončarić, Zdenko Kerovec, Darko Galović, Olivera Drenjančević, Ines Nutrients Article Objective: The present study aimed to evaluate the effects of enriched hen egg consumption on endothelium-dependent vasodilation (EDV) and the role of cyclooxygenases in EDV in the microcirculation of young healthy individuals. This study hypothesizes that Nutri4 eggs will improve endothelial function, which will be manifested by changes in microcirculatory flow measured by a laser Doppler flowmeter (LDF) during reactive hyperemia in response to vascular occlusion, in which n-3 PUFA plays an important role as well as its degradation pathway by cyclooxygenases. Materials and Methods: Participants consumed three eggs per day for three weeks: The control group (CTRL, n = 14) consumed regular hen eggs (approximately 0.330 mg of lutein, 1.785 mg of vitamin E, 0.054 mg of selenium and 438 mg of n-3 PUFAs daily) and Nutri4 group (n = 20) consumed enriched eggs (approximately 1.85 mg of lutein, 0.06 mg of selenium, 3.29 mg of vitamin E, and 1026 mg of n-3 PUFAs daily). Skin microvascular blood flow in response to EDV (post-occlusive reactive hyperemia (PORH) and iontophoresis of acetylcholine (AChID)) and sodium nitroprusside (SNPID; endothelium-independent) was assessed by laser Doppler flowmetry before and after dietary protocol and in a separate group of participants who were administered perorally 100 mg of indomethacin before microvascular response assessment. Arterial blood pressure, heart rate, serum lipid, and liver enzymes, anthropometric measurements, protein expression of cyclooxygenase 1 (COX-1), cyclooxygenase 2 (COX-2), neuronal nitric oxide synthases (nNOS), inducible nitric oxide synthases (iNOS), and endothelial nitric oxide synthases (eNOS) were measured before and after dietary protocol. Results: PORH and AChID were significantly enhanced, and SNPID remained unchanged in the Nutri4 group, while none was changed in the CTRL following a respective diet. PORH decreased after administration of indomethacin in Nutri4 after dietary protocol. Protein expression of COX-2 was significantly higher in the Nutri4 group compared to the CTRL after the dietary protocol. Conclusion: Consumption of enriched eggs improves microvascular EDV in healthy young subjects. Results suggest an element of n-3 PUFAs metabolites via the cyclooxygenases pathway in enhanced reactive hyperemia. MDPI 2023-03-25 /pmc/articles/PMC10097130/ /pubmed/37049437 http://dx.doi.org/10.3390/nu15071599 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Šušnjara, Petar
Mihaljević, Zrinka
Stupin, Ana
Kolobarić, Nikolina
Matić, Anita
Jukić, Ivana
Kralik, Zlata
Kralik, Gordana
Miloloža, Anđelina
Pavošević, Tihana
Šerić, Vatroslav
Lončarić, Zdenko
Kerovec, Darko
Galović, Olivera
Drenjančević, Ines
Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study
title Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study
title_full Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study
title_fullStr Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study
title_full_unstemmed Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study
title_short Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study
title_sort consumption of nutritionally enriched hen eggs enhances endothelium-dependent vasodilation via cyclooxygenase metabolites in healthy young people—a randomized study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097130/
https://www.ncbi.nlm.nih.gov/pubmed/37049437
http://dx.doi.org/10.3390/nu15071599
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