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Convolvulus pluricaulis Choisy’s Extraction, Chemical Characterization and Evaluation of the Potential Effects on Glycaemic Balance in a 3T3-L1 Adipocyte Cell Model

Convolvulus pluricaulis (CP) is a common Indian herb, largely employed in Ayurvedic medicine and known for its neuroprotective and neuroinflammatory action. Its effectiveness against several pathologic/sub-pathologic conditions is widely accepted, but it is not yet completely chemically characterize...

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Autores principales: Melloni, Elisabetta, Grassilli, Silvia, Romani, Arianna, Rimondi, Erika, Marcuzzi, Annalisa, Zauli, Enrico, Secchiero, Paola, Paganetto, Guglielmo, Guerrini, Alessandra, Sacchetti, Gianni, Tacchini, Massimo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097163/
https://www.ncbi.nlm.nih.gov/pubmed/37049568
http://dx.doi.org/10.3390/nu15071727
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author Melloni, Elisabetta
Grassilli, Silvia
Romani, Arianna
Rimondi, Erika
Marcuzzi, Annalisa
Zauli, Enrico
Secchiero, Paola
Paganetto, Guglielmo
Guerrini, Alessandra
Sacchetti, Gianni
Tacchini, Massimo
author_facet Melloni, Elisabetta
Grassilli, Silvia
Romani, Arianna
Rimondi, Erika
Marcuzzi, Annalisa
Zauli, Enrico
Secchiero, Paola
Paganetto, Guglielmo
Guerrini, Alessandra
Sacchetti, Gianni
Tacchini, Massimo
author_sort Melloni, Elisabetta
collection PubMed
description Convolvulus pluricaulis (CP) is a common Indian herb, largely employed in Ayurvedic medicine and known for its neuroprotective and neuroinflammatory action. Its effectiveness against several pathologic/sub-pathologic conditions is widely accepted, but it is not yet completely chemically characterized. In recent years, several researchers have pointed out the involvement of CP and other Convolvulaceae in lipidic and glucidic metabolism, particularly in the control of hyperlipidaemia and diabetic conditions. In this scenario, the aim of the study was to chemically characterize the medium polarity part of the CP whole plant and its fractions and to shed light on their biological activity in adipocyte differentiation using the 3T3-L1 cell model. Our results demonstrated that the CP extract and fractions could upregulate the adipocyte differentiation through the modulation of the nuclear receptor PPARγ (Peroxisome Proliferator-Activated Receptor γ), broadly recognized as a key regulator of adipocyte differentiation, and the glucose transporter GLUT-4, which is fundamental for cellular glucose uptake and for metabolism control. CP also showed the ability to exert an anti-inflammatory effect, downregulating cytokines such as Rantes, MCP-1, KC, eotaxin, and GM-CSF, which are deeply involved in insulin resistance and glucose intolerance. Taken together, these data suggest that CP could exert a potential beneficial effect on glycemia and could be employed as an anti-diabetic adjuvant or, in any case, a means to better control glucose homeostasis.
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spelling pubmed-100971632023-04-13 Convolvulus pluricaulis Choisy’s Extraction, Chemical Characterization and Evaluation of the Potential Effects on Glycaemic Balance in a 3T3-L1 Adipocyte Cell Model Melloni, Elisabetta Grassilli, Silvia Romani, Arianna Rimondi, Erika Marcuzzi, Annalisa Zauli, Enrico Secchiero, Paola Paganetto, Guglielmo Guerrini, Alessandra Sacchetti, Gianni Tacchini, Massimo Nutrients Article Convolvulus pluricaulis (CP) is a common Indian herb, largely employed in Ayurvedic medicine and known for its neuroprotective and neuroinflammatory action. Its effectiveness against several pathologic/sub-pathologic conditions is widely accepted, but it is not yet completely chemically characterized. In recent years, several researchers have pointed out the involvement of CP and other Convolvulaceae in lipidic and glucidic metabolism, particularly in the control of hyperlipidaemia and diabetic conditions. In this scenario, the aim of the study was to chemically characterize the medium polarity part of the CP whole plant and its fractions and to shed light on their biological activity in adipocyte differentiation using the 3T3-L1 cell model. Our results demonstrated that the CP extract and fractions could upregulate the adipocyte differentiation through the modulation of the nuclear receptor PPARγ (Peroxisome Proliferator-Activated Receptor γ), broadly recognized as a key regulator of adipocyte differentiation, and the glucose transporter GLUT-4, which is fundamental for cellular glucose uptake and for metabolism control. CP also showed the ability to exert an anti-inflammatory effect, downregulating cytokines such as Rantes, MCP-1, KC, eotaxin, and GM-CSF, which are deeply involved in insulin resistance and glucose intolerance. Taken together, these data suggest that CP could exert a potential beneficial effect on glycemia and could be employed as an anti-diabetic adjuvant or, in any case, a means to better control glucose homeostasis. MDPI 2023-03-31 /pmc/articles/PMC10097163/ /pubmed/37049568 http://dx.doi.org/10.3390/nu15071727 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Melloni, Elisabetta
Grassilli, Silvia
Romani, Arianna
Rimondi, Erika
Marcuzzi, Annalisa
Zauli, Enrico
Secchiero, Paola
Paganetto, Guglielmo
Guerrini, Alessandra
Sacchetti, Gianni
Tacchini, Massimo
Convolvulus pluricaulis Choisy’s Extraction, Chemical Characterization and Evaluation of the Potential Effects on Glycaemic Balance in a 3T3-L1 Adipocyte Cell Model
title Convolvulus pluricaulis Choisy’s Extraction, Chemical Characterization and Evaluation of the Potential Effects on Glycaemic Balance in a 3T3-L1 Adipocyte Cell Model
title_full Convolvulus pluricaulis Choisy’s Extraction, Chemical Characterization and Evaluation of the Potential Effects on Glycaemic Balance in a 3T3-L1 Adipocyte Cell Model
title_fullStr Convolvulus pluricaulis Choisy’s Extraction, Chemical Characterization and Evaluation of the Potential Effects on Glycaemic Balance in a 3T3-L1 Adipocyte Cell Model
title_full_unstemmed Convolvulus pluricaulis Choisy’s Extraction, Chemical Characterization and Evaluation of the Potential Effects on Glycaemic Balance in a 3T3-L1 Adipocyte Cell Model
title_short Convolvulus pluricaulis Choisy’s Extraction, Chemical Characterization and Evaluation of the Potential Effects on Glycaemic Balance in a 3T3-L1 Adipocyte Cell Model
title_sort convolvulus pluricaulis choisy’s extraction, chemical characterization and evaluation of the potential effects on glycaemic balance in a 3t3-l1 adipocyte cell model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097163/
https://www.ncbi.nlm.nih.gov/pubmed/37049568
http://dx.doi.org/10.3390/nu15071727
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