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Fingerprinting of Plum (Prunus domestica) Genotypes in Lithuania Using SSR Markers
This study’s aim was to evaluate the genetic diversity of European plum (Prunus domestica) cultivars and hybrids in Lithuania using SSR markers. In total, 107 plum genotypes (including 68 European plum cultivars and 39 hybrids) from the genetic resources collection of the Institute of Horticulture o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097231/ https://www.ncbi.nlm.nih.gov/pubmed/37050164 http://dx.doi.org/10.3390/plants12071538 |
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author | Antanynienė, Raminta Šikšnianienė, Jūratė Bronė Stanys, Vidmantas Frercks, Birutė |
author_facet | Antanynienė, Raminta Šikšnianienė, Jūratė Bronė Stanys, Vidmantas Frercks, Birutė |
author_sort | Antanynienė, Raminta |
collection | PubMed |
description | This study’s aim was to evaluate the genetic diversity of European plum (Prunus domestica) cultivars and hybrids in Lithuania using SSR markers. In total, 107 plum genotypes (including 68 European plum cultivars and 39 hybrids) from the genetic resources collection of the Institute of Horticulture of the Lithuanian Research Centre for Agriculture and Forestry (LRCAF IH) were evaluated using nine microsatellite markers (SSRs) previously published and suggested by the European Cooperative Programme for Plant Genetic Resources (ECPGR). Up to six alleles per locus with each primer pair were generated for some genotypes due to the hexaploidy of plums. The number of alleles in each primer ranged from 18 to 30, with an average of 24.33. The highest number of alleles was generated with the PacA33 primer pair (30). The most informative primer, according to the PIC value, was BPPCT007. Sixty-two unique alleles (representing 39.5% of all polymorphic alleles) have been detected in the plum germplasm developed in Lithuania. According to UPGMA cluster analysis, 58 European plum genotypes were separated into eight groups without any relation to fruit color or shape. By genetic diversity (UPGMA) and structure (Bayesian) analysis, European plum hybrids were grouped into clusters according to their pedigree. |
format | Online Article Text |
id | pubmed-10097231 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100972312023-04-13 Fingerprinting of Plum (Prunus domestica) Genotypes in Lithuania Using SSR Markers Antanynienė, Raminta Šikšnianienė, Jūratė Bronė Stanys, Vidmantas Frercks, Birutė Plants (Basel) Article This study’s aim was to evaluate the genetic diversity of European plum (Prunus domestica) cultivars and hybrids in Lithuania using SSR markers. In total, 107 plum genotypes (including 68 European plum cultivars and 39 hybrids) from the genetic resources collection of the Institute of Horticulture of the Lithuanian Research Centre for Agriculture and Forestry (LRCAF IH) were evaluated using nine microsatellite markers (SSRs) previously published and suggested by the European Cooperative Programme for Plant Genetic Resources (ECPGR). Up to six alleles per locus with each primer pair were generated for some genotypes due to the hexaploidy of plums. The number of alleles in each primer ranged from 18 to 30, with an average of 24.33. The highest number of alleles was generated with the PacA33 primer pair (30). The most informative primer, according to the PIC value, was BPPCT007. Sixty-two unique alleles (representing 39.5% of all polymorphic alleles) have been detected in the plum germplasm developed in Lithuania. According to UPGMA cluster analysis, 58 European plum genotypes were separated into eight groups without any relation to fruit color or shape. By genetic diversity (UPGMA) and structure (Bayesian) analysis, European plum hybrids were grouped into clusters according to their pedigree. MDPI 2023-04-03 /pmc/articles/PMC10097231/ /pubmed/37050164 http://dx.doi.org/10.3390/plants12071538 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Antanynienė, Raminta Šikšnianienė, Jūratė Bronė Stanys, Vidmantas Frercks, Birutė Fingerprinting of Plum (Prunus domestica) Genotypes in Lithuania Using SSR Markers |
title | Fingerprinting of Plum (Prunus domestica) Genotypes in Lithuania Using SSR Markers |
title_full | Fingerprinting of Plum (Prunus domestica) Genotypes in Lithuania Using SSR Markers |
title_fullStr | Fingerprinting of Plum (Prunus domestica) Genotypes in Lithuania Using SSR Markers |
title_full_unstemmed | Fingerprinting of Plum (Prunus domestica) Genotypes in Lithuania Using SSR Markers |
title_short | Fingerprinting of Plum (Prunus domestica) Genotypes in Lithuania Using SSR Markers |
title_sort | fingerprinting of plum (prunus domestica) genotypes in lithuania using ssr markers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097231/ https://www.ncbi.nlm.nih.gov/pubmed/37050164 http://dx.doi.org/10.3390/plants12071538 |
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