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Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation

Functional constipation (FC) is a gastrointestinal disorder with high incidence, and it seriously affects patients’ physical and mental health. Several studies have shown that the gut microbiome is associated with FC, but these studies have produced inconsistent findings, with few reflecting the rel...

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Autores principales: Wang, Jialiang, Wang, Linlin, Yu, Qiangqing, Tang, Nan, Mei, Chunxia, Zhang, Hao, Wang, Gang, Lu, Jian, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097253/
https://www.ncbi.nlm.nih.gov/pubmed/37049619
http://dx.doi.org/10.3390/nu15071779
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author Wang, Jialiang
Wang, Linlin
Yu, Qiangqing
Tang, Nan
Mei, Chunxia
Zhang, Hao
Wang, Gang
Lu, Jian
Chen, Wei
author_facet Wang, Jialiang
Wang, Linlin
Yu, Qiangqing
Tang, Nan
Mei, Chunxia
Zhang, Hao
Wang, Gang
Lu, Jian
Chen, Wei
author_sort Wang, Jialiang
collection PubMed
description Functional constipation (FC) is a gastrointestinal disorder with high incidence, and it seriously affects patients’ physical and mental health. Several studies have shown that the gut microbiome is associated with FC, but these studies have produced inconsistent findings, with few reflecting the relationship between the gut microbiome and metabolites. This study used 16S rRNA microbial genomics and non-target metabolome based on liquid chromatography–mass spectrometry to analyze the gut microbiota composition and serum metabolic profiles of 30 FC patients and 28 healthy individuals. We found that patients with FC and healthy individuals have different gut microbiota structures and serum metabolic profiles. FC patients had more Bacteroides and butyrate-producing bacteria (Roseburia, Faecaliberium, Butyriccoccus). The upstream products of host arginine biosynthesis (2-oxoglutaric acid, L-glutamic acid, N-acetylornithine, and L-ornithine) were significantly reduced in FC patients’ serum metabolites. In summary, our study describes the gut microbiome and serum metabolome of patients with functional constipation. It reveals that functional constipation may be associated with increased Bacteroidetes and downregulation of upstream products of host arginine biosynthesis, which may be potential markers for diagnosing functional constipation.
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spelling pubmed-100972532023-04-13 Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation Wang, Jialiang Wang, Linlin Yu, Qiangqing Tang, Nan Mei, Chunxia Zhang, Hao Wang, Gang Lu, Jian Chen, Wei Nutrients Article Functional constipation (FC) is a gastrointestinal disorder with high incidence, and it seriously affects patients’ physical and mental health. Several studies have shown that the gut microbiome is associated with FC, but these studies have produced inconsistent findings, with few reflecting the relationship between the gut microbiome and metabolites. This study used 16S rRNA microbial genomics and non-target metabolome based on liquid chromatography–mass spectrometry to analyze the gut microbiota composition and serum metabolic profiles of 30 FC patients and 28 healthy individuals. We found that patients with FC and healthy individuals have different gut microbiota structures and serum metabolic profiles. FC patients had more Bacteroides and butyrate-producing bacteria (Roseburia, Faecaliberium, Butyriccoccus). The upstream products of host arginine biosynthesis (2-oxoglutaric acid, L-glutamic acid, N-acetylornithine, and L-ornithine) were significantly reduced in FC patients’ serum metabolites. In summary, our study describes the gut microbiome and serum metabolome of patients with functional constipation. It reveals that functional constipation may be associated with increased Bacteroidetes and downregulation of upstream products of host arginine biosynthesis, which may be potential markers for diagnosing functional constipation. MDPI 2023-04-06 /pmc/articles/PMC10097253/ /pubmed/37049619 http://dx.doi.org/10.3390/nu15071779 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Jialiang
Wang, Linlin
Yu, Qiangqing
Tang, Nan
Mei, Chunxia
Zhang, Hao
Wang, Gang
Lu, Jian
Chen, Wei
Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation
title Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation
title_full Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation
title_fullStr Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation
title_full_unstemmed Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation
title_short Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation
title_sort characteristics of the gut microbiome and serum metabolome in patients with functional constipation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097253/
https://www.ncbi.nlm.nih.gov/pubmed/37049619
http://dx.doi.org/10.3390/nu15071779
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