Cargando…

Goss’s Wilt Resistance in Corn Is Mediated via Salicylic Acid and Programmed Cell Death but Not Jasmonic Acid Pathways

A highly aggressive strain (CMN14-5-1) of Clavibacter nebraskensis bacteria, which causes Goss’s wilt in corn, induced severe symptoms in a susceptible corn line (CO447), resulting in water-soaked lesions followed by necrosis within a few days. A tolerant line (CO450) inoculated with the same strain...

Descripción completa

Detalles Bibliográficos
Autores principales: Shumilak, Alexander, El-Shetehy, Mohamed, Soliman, Atta, Tambong, James T., Daayf, Fouad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097360/
https://www.ncbi.nlm.nih.gov/pubmed/37050101
http://dx.doi.org/10.3390/plants12071475
_version_ 1785024561952063488
author Shumilak, Alexander
El-Shetehy, Mohamed
Soliman, Atta
Tambong, James T.
Daayf, Fouad
author_facet Shumilak, Alexander
El-Shetehy, Mohamed
Soliman, Atta
Tambong, James T.
Daayf, Fouad
author_sort Shumilak, Alexander
collection PubMed
description A highly aggressive strain (CMN14-5-1) of Clavibacter nebraskensis bacteria, which causes Goss’s wilt in corn, induced severe symptoms in a susceptible corn line (CO447), resulting in water-soaked lesions followed by necrosis within a few days. A tolerant line (CO450) inoculated with the same strain exhibited only mild symptoms such as chlorosis, freckling, and necrosis that did not progress after the first six days following infection. Both lesion length and disease severity were measured using the area under the disease progression curve (AUDPC), and significant differences were found between treatments. We analyzed the expression of key genes related to plant defense in both corn lines challenged with the CMN14-5-1 strain. Allene oxide synthase (ZmAOS), a gene responsible for the production of jasmonic acid (JA), was induced in the CO447 line in response to CMN14-5-1. Following inoculation with CMN14-5-1, the CO450 line demonstrated a higher expression of salicylic acid (SA)-related genes, ZmPAL and ZmPR-1, compared to the CO447 line. In the CO450 line, four genes related to programmed cell death (PCD) were upregulated: respiratory burst oxidase homolog protein D (ZmrbohD), polyphenol oxidase (ZmPPO1), ras-related protein 7 (ZmRab7), and peptidyl-prolyl cis-trans isomerase (ZmPPI). The differential gene expression in response to CMN14-5-1 between the two corn lines provided an indication that SA and PCD are involved in the regulation of corn defense responses against Goss’s wilt disease, whereas JA may be contributing to disease susceptibility.
format Online
Article
Text
id pubmed-10097360
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100973602023-04-13 Goss’s Wilt Resistance in Corn Is Mediated via Salicylic Acid and Programmed Cell Death but Not Jasmonic Acid Pathways Shumilak, Alexander El-Shetehy, Mohamed Soliman, Atta Tambong, James T. Daayf, Fouad Plants (Basel) Article A highly aggressive strain (CMN14-5-1) of Clavibacter nebraskensis bacteria, which causes Goss’s wilt in corn, induced severe symptoms in a susceptible corn line (CO447), resulting in water-soaked lesions followed by necrosis within a few days. A tolerant line (CO450) inoculated with the same strain exhibited only mild symptoms such as chlorosis, freckling, and necrosis that did not progress after the first six days following infection. Both lesion length and disease severity were measured using the area under the disease progression curve (AUDPC), and significant differences were found between treatments. We analyzed the expression of key genes related to plant defense in both corn lines challenged with the CMN14-5-1 strain. Allene oxide synthase (ZmAOS), a gene responsible for the production of jasmonic acid (JA), was induced in the CO447 line in response to CMN14-5-1. Following inoculation with CMN14-5-1, the CO450 line demonstrated a higher expression of salicylic acid (SA)-related genes, ZmPAL and ZmPR-1, compared to the CO447 line. In the CO450 line, four genes related to programmed cell death (PCD) were upregulated: respiratory burst oxidase homolog protein D (ZmrbohD), polyphenol oxidase (ZmPPO1), ras-related protein 7 (ZmRab7), and peptidyl-prolyl cis-trans isomerase (ZmPPI). The differential gene expression in response to CMN14-5-1 between the two corn lines provided an indication that SA and PCD are involved in the regulation of corn defense responses against Goss’s wilt disease, whereas JA may be contributing to disease susceptibility. MDPI 2023-03-28 /pmc/articles/PMC10097360/ /pubmed/37050101 http://dx.doi.org/10.3390/plants12071475 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shumilak, Alexander
El-Shetehy, Mohamed
Soliman, Atta
Tambong, James T.
Daayf, Fouad
Goss’s Wilt Resistance in Corn Is Mediated via Salicylic Acid and Programmed Cell Death but Not Jasmonic Acid Pathways
title Goss’s Wilt Resistance in Corn Is Mediated via Salicylic Acid and Programmed Cell Death but Not Jasmonic Acid Pathways
title_full Goss’s Wilt Resistance in Corn Is Mediated via Salicylic Acid and Programmed Cell Death but Not Jasmonic Acid Pathways
title_fullStr Goss’s Wilt Resistance in Corn Is Mediated via Salicylic Acid and Programmed Cell Death but Not Jasmonic Acid Pathways
title_full_unstemmed Goss’s Wilt Resistance in Corn Is Mediated via Salicylic Acid and Programmed Cell Death but Not Jasmonic Acid Pathways
title_short Goss’s Wilt Resistance in Corn Is Mediated via Salicylic Acid and Programmed Cell Death but Not Jasmonic Acid Pathways
title_sort goss’s wilt resistance in corn is mediated via salicylic acid and programmed cell death but not jasmonic acid pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097360/
https://www.ncbi.nlm.nih.gov/pubmed/37050101
http://dx.doi.org/10.3390/plants12071475
work_keys_str_mv AT shumilakalexander gossswiltresistanceincornismediatedviasalicylicacidandprogrammedcelldeathbutnotjasmonicacidpathways
AT elshetehymohamed gossswiltresistanceincornismediatedviasalicylicacidandprogrammedcelldeathbutnotjasmonicacidpathways
AT solimanatta gossswiltresistanceincornismediatedviasalicylicacidandprogrammedcelldeathbutnotjasmonicacidpathways
AT tambongjamest gossswiltresistanceincornismediatedviasalicylicacidandprogrammedcelldeathbutnotjasmonicacidpathways
AT daayffouad gossswiltresistanceincornismediatedviasalicylicacidandprogrammedcelldeathbutnotjasmonicacidpathways