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Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus
Tomato yellow leaf curl virus (TYLCV) is a global spreading begomovirus that is exerting a major restraint on global tomato production. In this transgenic approach, an RNA interference (RNAi)-based construct consisting of sequences of an artificial microRNA (amiRNA), a group of small RNA molecules n...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098008/ https://www.ncbi.nlm.nih.gov/pubmed/37063229 http://dx.doi.org/10.3389/fpls.2023.1164921 |
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author | Al-Roshdi, Maha R. Ammara, Ume Khan, Jamal Al-Sadi, Abdullah M. Shahid, Muhammad Shafiq |
author_facet | Al-Roshdi, Maha R. Ammara, Ume Khan, Jamal Al-Sadi, Abdullah M. Shahid, Muhammad Shafiq |
author_sort | Al-Roshdi, Maha R. |
collection | PubMed |
description | Tomato yellow leaf curl virus (TYLCV) is a global spreading begomovirus that is exerting a major restraint on global tomato production. In this transgenic approach, an RNA interference (RNAi)-based construct consisting of sequences of an artificial microRNA (amiRNA), a group of small RNA molecules necessary for plant cell development, signal transduction, and stimulus to biotic and abiotic disease was engineered targeting the AC1/Rep gene of the Oman strain of TYLCV-OM. The Rep-amiRNA constructs presented an effective approach in regulating the expression of the Rep gene against TYLCV as a silencing target to create transgenic Solanum lycopersicum L. plant tolerance against TYLCV infection. Molecular diagnosis by PCR followed by a Southern hybridization analysis were performed to confirm the effectiveness of agrobacterium-mediated transformation in T0/T1-transformed plants. A substantial decrease in virus replication was observed when T1 transgenic tomato plants were challenged with the TYLCV-OM infectious construct. Although natural resistance options against TYLCV infection are not accessible, the current study proposes that genetically transformed tomato plants expressing amiRNA could be a potential approach for engineering tolerance in plants against TYLCV infection and conceivably for the inhibition of viral diseases against different strains of whitefly-transmitted begomoviruses in Oman. |
format | Online Article Text |
id | pubmed-10098008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100980082023-04-14 Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus Al-Roshdi, Maha R. Ammara, Ume Khan, Jamal Al-Sadi, Abdullah M. Shahid, Muhammad Shafiq Front Plant Sci Plant Science Tomato yellow leaf curl virus (TYLCV) is a global spreading begomovirus that is exerting a major restraint on global tomato production. In this transgenic approach, an RNA interference (RNAi)-based construct consisting of sequences of an artificial microRNA (amiRNA), a group of small RNA molecules necessary for plant cell development, signal transduction, and stimulus to biotic and abiotic disease was engineered targeting the AC1/Rep gene of the Oman strain of TYLCV-OM. The Rep-amiRNA constructs presented an effective approach in regulating the expression of the Rep gene against TYLCV as a silencing target to create transgenic Solanum lycopersicum L. plant tolerance against TYLCV infection. Molecular diagnosis by PCR followed by a Southern hybridization analysis were performed to confirm the effectiveness of agrobacterium-mediated transformation in T0/T1-transformed plants. A substantial decrease in virus replication was observed when T1 transgenic tomato plants were challenged with the TYLCV-OM infectious construct. Although natural resistance options against TYLCV infection are not accessible, the current study proposes that genetically transformed tomato plants expressing amiRNA could be a potential approach for engineering tolerance in plants against TYLCV infection and conceivably for the inhibition of viral diseases against different strains of whitefly-transmitted begomoviruses in Oman. Frontiers Media S.A. 2023-03-30 /pmc/articles/PMC10098008/ /pubmed/37063229 http://dx.doi.org/10.3389/fpls.2023.1164921 Text en Copyright © 2023 Al-Roshdi, Ammara, Khan, Al-Sadi and Shahid https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Al-Roshdi, Maha R. Ammara, Ume Khan, Jamal Al-Sadi, Abdullah M. Shahid, Muhammad Shafiq Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus |
title | Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus |
title_full | Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus |
title_fullStr | Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus |
title_full_unstemmed | Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus |
title_short | Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus |
title_sort | artificial microrna-mediated resistance against oman strain of tomato yellow leaf curl virus |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098008/ https://www.ncbi.nlm.nih.gov/pubmed/37063229 http://dx.doi.org/10.3389/fpls.2023.1164921 |
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