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MicroRNA‐143‐3p ameliorates collagen‐induced arthritis by polarizing naive CD4 (+)T cells into Treg cells

BACKGROUND: Rheumatoid arthritis (RA) is a persistent and systemic autoimmunity disease. The abnormal differentiation of Treg cells is important in pathogenesis. Despite previous studies showed that microRNAs (miRNAs, miR) are pivotal modulators of Treg cells, the effect of miRNAs on Treg cell diffe...

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Autores principales: Shen, Mei‐Yu, Jiang, Bao‐Ping, Zhang, Ming‐Fei, Wang, Xiang, Zhu, Hong, Gu, Zhen‐Ning, Zhou, Xue‐Ping, Lu, Yan, Zhou, Ling‐Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098049/
https://www.ncbi.nlm.nih.gov/pubmed/36861291
http://dx.doi.org/10.1002/jcla.24845
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author Shen, Mei‐Yu
Jiang, Bao‐Ping
Zhang, Ming‐Fei
Wang, Xiang
Zhu, Hong
Gu, Zhen‐Ning
Zhou, Xue‐Ping
Lu, Yan
Zhou, Ling‐Ling
author_facet Shen, Mei‐Yu
Jiang, Bao‐Ping
Zhang, Ming‐Fei
Wang, Xiang
Zhu, Hong
Gu, Zhen‐Ning
Zhou, Xue‐Ping
Lu, Yan
Zhou, Ling‐Ling
author_sort Shen, Mei‐Yu
collection PubMed
description BACKGROUND: Rheumatoid arthritis (RA) is a persistent and systemic autoimmunity disease. The abnormal differentiation of Treg cells is important in pathogenesis. Despite previous studies showed that microRNAs (miRNAs, miR) are pivotal modulators of Treg cells, the effect of miRNAs on Treg cell differentiation and function is not clear. Our study wants to reveal the relationship of miR‐143‐3p with the differentiative ability and biofunction of Treg cells during the development of RA. METHODS: The Expressing level of miR‐143‐3p and cell factor generation in peripheral blood (PB) of RA sufferers were identified by ELISA or RT‐qPCR. The roles of miR‐143‐3p in Treg cell differentiation were studied via ShRNA/lentivirus transfection. Male DBA/1 J mice were separated into control, model, control mimics, and miR‐143‐3p mimics groups to analyze the anti‐arthritis efficacy, the differentiative ability of Treg cells, and the expression level of miR‐143‐3p. RESULTS: Our team discovered that the Expressing level of miR‐143‐3p was related to RA disease activities in a negative manner, and remarkably related to antiinflammation cell factor IL‐10. In vitro, the expression of miR‐143‐3p in the CD4(+)T cells upregulated the percentage of CD4(+)CD25(+)Fxop3(+) cells (Tregs) and forkhead box protein 3 (Foxp3) mRNA expression. Evidently, miR‐143‐3p mimic intervention considerably upregulated the content of Treg cells in vivo, validly avoided CIA progression, and remarkably suppressed the inflammatory events of joints in mice. CONCLUSION: Our findings indicated that miR‐143‐3p could ameliorate CIA through polarizing naive CD4(+)T cells into Treg cells, which may be a novel strategy to treat autoimmune diseases such as RA.
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spelling pubmed-100980492023-04-14 MicroRNA‐143‐3p ameliorates collagen‐induced arthritis by polarizing naive CD4 (+)T cells into Treg cells Shen, Mei‐Yu Jiang, Bao‐Ping Zhang, Ming‐Fei Wang, Xiang Zhu, Hong Gu, Zhen‐Ning Zhou, Xue‐Ping Lu, Yan Zhou, Ling‐Ling J Clin Lab Anal Research Articles BACKGROUND: Rheumatoid arthritis (RA) is a persistent and systemic autoimmunity disease. The abnormal differentiation of Treg cells is important in pathogenesis. Despite previous studies showed that microRNAs (miRNAs, miR) are pivotal modulators of Treg cells, the effect of miRNAs on Treg cell differentiation and function is not clear. Our study wants to reveal the relationship of miR‐143‐3p with the differentiative ability and biofunction of Treg cells during the development of RA. METHODS: The Expressing level of miR‐143‐3p and cell factor generation in peripheral blood (PB) of RA sufferers were identified by ELISA or RT‐qPCR. The roles of miR‐143‐3p in Treg cell differentiation were studied via ShRNA/lentivirus transfection. Male DBA/1 J mice were separated into control, model, control mimics, and miR‐143‐3p mimics groups to analyze the anti‐arthritis efficacy, the differentiative ability of Treg cells, and the expression level of miR‐143‐3p. RESULTS: Our team discovered that the Expressing level of miR‐143‐3p was related to RA disease activities in a negative manner, and remarkably related to antiinflammation cell factor IL‐10. In vitro, the expression of miR‐143‐3p in the CD4(+)T cells upregulated the percentage of CD4(+)CD25(+)Fxop3(+) cells (Tregs) and forkhead box protein 3 (Foxp3) mRNA expression. Evidently, miR‐143‐3p mimic intervention considerably upregulated the content of Treg cells in vivo, validly avoided CIA progression, and remarkably suppressed the inflammatory events of joints in mice. CONCLUSION: Our findings indicated that miR‐143‐3p could ameliorate CIA through polarizing naive CD4(+)T cells into Treg cells, which may be a novel strategy to treat autoimmune diseases such as RA. John Wiley and Sons Inc. 2023-03-01 /pmc/articles/PMC10098049/ /pubmed/36861291 http://dx.doi.org/10.1002/jcla.24845 Text en © 2023 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Shen, Mei‐Yu
Jiang, Bao‐Ping
Zhang, Ming‐Fei
Wang, Xiang
Zhu, Hong
Gu, Zhen‐Ning
Zhou, Xue‐Ping
Lu, Yan
Zhou, Ling‐Ling
MicroRNA‐143‐3p ameliorates collagen‐induced arthritis by polarizing naive CD4 (+)T cells into Treg cells
title MicroRNA‐143‐3p ameliorates collagen‐induced arthritis by polarizing naive CD4 (+)T cells into Treg cells
title_full MicroRNA‐143‐3p ameliorates collagen‐induced arthritis by polarizing naive CD4 (+)T cells into Treg cells
title_fullStr MicroRNA‐143‐3p ameliorates collagen‐induced arthritis by polarizing naive CD4 (+)T cells into Treg cells
title_full_unstemmed MicroRNA‐143‐3p ameliorates collagen‐induced arthritis by polarizing naive CD4 (+)T cells into Treg cells
title_short MicroRNA‐143‐3p ameliorates collagen‐induced arthritis by polarizing naive CD4 (+)T cells into Treg cells
title_sort microrna‐143‐3p ameliorates collagen‐induced arthritis by polarizing naive cd4 (+)t cells into treg cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098049/
https://www.ncbi.nlm.nih.gov/pubmed/36861291
http://dx.doi.org/10.1002/jcla.24845
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