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Fullerenol inhibits tendinopathy by alleviating inflammation
Tendinopathy is a common disease in orthopaedics, seriously affecting tendon functions. However, the effects of non-surgical treatment on tendinopathy are not satisfactory and surgical treatments possibly impair the function of tendons. Biomaterial fullerenol has been proved to show good anti-inflam...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098086/ https://www.ncbi.nlm.nih.gov/pubmed/37064249 http://dx.doi.org/10.3389/fbioe.2023.1171360 |
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author | Jiao, Xin Wang, Zengguang Li, Yiming Wang, Tianchang Xu, Chen Zhou, Xianhao Gan, Yaokai |
author_facet | Jiao, Xin Wang, Zengguang Li, Yiming Wang, Tianchang Xu, Chen Zhou, Xianhao Gan, Yaokai |
author_sort | Jiao, Xin |
collection | PubMed |
description | Tendinopathy is a common disease in orthopaedics, seriously affecting tendon functions. However, the effects of non-surgical treatment on tendinopathy are not satisfactory and surgical treatments possibly impair the function of tendons. Biomaterial fullerenol has been proved to show good anti-inflammatory effects on various inflammatory diseases. For in vitro experiments, primary rat tendon cells (TCs) were treated by interleukin-1 beta (IL-1β) combined with aqueous fullerenol (5, 1, 0.3 μg/mL). Then inflammatory factors, tendon-related markers, migration and signaling pathways were detected. For in vivo experiments, rat tendinopathy model was constructed by local injection of collagenase into Achilles tendons of rats and fullerenol (0.5, 1 mg/mL) was locally injected 7 days after collagenase injection. Inflammatory factors and tendon-related markers were also investigated. Fullerenol with good water-solubility showed excellent biocompatibility with TCs. Fullerenol could increase expression of tendon-related factors (Collagen I and tenascin C) and decrease expression of inflammatory factors (matrix metalloproteinases-3, MMP-3, and MMP-13) and reactive oxygen species (ROS) level. Simultaneously, fullerenol slowed the migration of TCs and inhibited activation of Mitogen-activated protein kinase (MAPK) signaling pathway. Fullerenol also attenuated tendinopathy in vivo, including reduction of fiber disorders, decrease of inflammatory factors and increase of tendon markers. In summary, fullerenol is a promising biomaterial that can be used to treat tendinopathy. |
format | Online Article Text |
id | pubmed-10098086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100980862023-04-14 Fullerenol inhibits tendinopathy by alleviating inflammation Jiao, Xin Wang, Zengguang Li, Yiming Wang, Tianchang Xu, Chen Zhou, Xianhao Gan, Yaokai Front Bioeng Biotechnol Bioengineering and Biotechnology Tendinopathy is a common disease in orthopaedics, seriously affecting tendon functions. However, the effects of non-surgical treatment on tendinopathy are not satisfactory and surgical treatments possibly impair the function of tendons. Biomaterial fullerenol has been proved to show good anti-inflammatory effects on various inflammatory diseases. For in vitro experiments, primary rat tendon cells (TCs) were treated by interleukin-1 beta (IL-1β) combined with aqueous fullerenol (5, 1, 0.3 μg/mL). Then inflammatory factors, tendon-related markers, migration and signaling pathways were detected. For in vivo experiments, rat tendinopathy model was constructed by local injection of collagenase into Achilles tendons of rats and fullerenol (0.5, 1 mg/mL) was locally injected 7 days after collagenase injection. Inflammatory factors and tendon-related markers were also investigated. Fullerenol with good water-solubility showed excellent biocompatibility with TCs. Fullerenol could increase expression of tendon-related factors (Collagen I and tenascin C) and decrease expression of inflammatory factors (matrix metalloproteinases-3, MMP-3, and MMP-13) and reactive oxygen species (ROS) level. Simultaneously, fullerenol slowed the migration of TCs and inhibited activation of Mitogen-activated protein kinase (MAPK) signaling pathway. Fullerenol also attenuated tendinopathy in vivo, including reduction of fiber disorders, decrease of inflammatory factors and increase of tendon markers. In summary, fullerenol is a promising biomaterial that can be used to treat tendinopathy. Frontiers Media S.A. 2023-03-30 /pmc/articles/PMC10098086/ /pubmed/37064249 http://dx.doi.org/10.3389/fbioe.2023.1171360 Text en Copyright © 2023 Jiao, Wang, Li, Wang, Xu, Zhou and Gan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Jiao, Xin Wang, Zengguang Li, Yiming Wang, Tianchang Xu, Chen Zhou, Xianhao Gan, Yaokai Fullerenol inhibits tendinopathy by alleviating inflammation |
title | Fullerenol inhibits tendinopathy by alleviating inflammation |
title_full | Fullerenol inhibits tendinopathy by alleviating inflammation |
title_fullStr | Fullerenol inhibits tendinopathy by alleviating inflammation |
title_full_unstemmed | Fullerenol inhibits tendinopathy by alleviating inflammation |
title_short | Fullerenol inhibits tendinopathy by alleviating inflammation |
title_sort | fullerenol inhibits tendinopathy by alleviating inflammation |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098086/ https://www.ncbi.nlm.nih.gov/pubmed/37064249 http://dx.doi.org/10.3389/fbioe.2023.1171360 |
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