Cargando…

Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation

Chitosan is a biopolymer material extracted from marine biomass waste such as shrimp and crab shells, which has good biocompatibility and degradability with great potential for application in the field of wastewater treatment and soil remediation. The higher the degree of deacetylation (DD), the bet...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yingying, Luo, Xi, Yin, Longfei, Yin, Fengwei, Zheng, Weilong, Fu, Yongqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098122/
https://www.ncbi.nlm.nih.gov/pubmed/37064228
http://dx.doi.org/10.3389/fbioe.2023.1183333
_version_ 1785024731799355392
author Zhang, Yingying
Luo, Xi
Yin, Longfei
Yin, Fengwei
Zheng, Weilong
Fu, Yongqian
author_facet Zhang, Yingying
Luo, Xi
Yin, Longfei
Yin, Fengwei
Zheng, Weilong
Fu, Yongqian
author_sort Zhang, Yingying
collection PubMed
description Chitosan is a biopolymer material extracted from marine biomass waste such as shrimp and crab shells, which has good biocompatibility and degradability with great potential for application in the field of wastewater treatment and soil remediation. The higher the degree of deacetylation (DD), the better the adsorption performance of chitosan. Chitin deacetylase (CDA) can specifically catalyze the deacetylate of chitin in a green reaction that is environmentally friendly. However, the scarcity of high yielding chitin deacetylase strains has been regarded as the technical bottleneck of chitosan green production. Here, we screened a natural chitin degrading bacterium from coastal mud and identified it as Bacillus cereus ZWT-08 by re-screening the chitin deacetylase activity and degree of deacetylation values. By optimizing the medium conditions and enzyme production process, ZWT-08 was cultured in fermentation medium with 1% (m/V) glucose and yeast extract at pH 6.0, 37°C, and a stirring speed of 180 r/min. After fermenting in 5 L fermenter for 48 h, the deacetylation activity of the supernatant reached 613.25 U/mL. Electron microscopic examination of the chitin substrate in the fermentation medium revealed a marshmallow-like fluffy texture on its structural surface. Meanwhile, 89.29% of the acetyl groups in this chitin substrate were removed by enzymatic digestion of chitin deacetylase produced by ZWT-08, resulting in the preparation of chitosan a degree of deacetylation higher than 90%. As an effective strain for chitosan production, Bacillus cereus ZWT-08 plays a positive role in the bioconversion of chitin and the upgrading of the chitosan industry.
format Online
Article
Text
id pubmed-10098122
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-100981222023-04-14 Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation Zhang, Yingying Luo, Xi Yin, Longfei Yin, Fengwei Zheng, Weilong Fu, Yongqian Front Bioeng Biotechnol Bioengineering and Biotechnology Chitosan is a biopolymer material extracted from marine biomass waste such as shrimp and crab shells, which has good biocompatibility and degradability with great potential for application in the field of wastewater treatment and soil remediation. The higher the degree of deacetylation (DD), the better the adsorption performance of chitosan. Chitin deacetylase (CDA) can specifically catalyze the deacetylate of chitin in a green reaction that is environmentally friendly. However, the scarcity of high yielding chitin deacetylase strains has been regarded as the technical bottleneck of chitosan green production. Here, we screened a natural chitin degrading bacterium from coastal mud and identified it as Bacillus cereus ZWT-08 by re-screening the chitin deacetylase activity and degree of deacetylation values. By optimizing the medium conditions and enzyme production process, ZWT-08 was cultured in fermentation medium with 1% (m/V) glucose and yeast extract at pH 6.0, 37°C, and a stirring speed of 180 r/min. After fermenting in 5 L fermenter for 48 h, the deacetylation activity of the supernatant reached 613.25 U/mL. Electron microscopic examination of the chitin substrate in the fermentation medium revealed a marshmallow-like fluffy texture on its structural surface. Meanwhile, 89.29% of the acetyl groups in this chitin substrate were removed by enzymatic digestion of chitin deacetylase produced by ZWT-08, resulting in the preparation of chitosan a degree of deacetylation higher than 90%. As an effective strain for chitosan production, Bacillus cereus ZWT-08 plays a positive role in the bioconversion of chitin and the upgrading of the chitosan industry. Frontiers Media S.A. 2023-03-30 /pmc/articles/PMC10098122/ /pubmed/37064228 http://dx.doi.org/10.3389/fbioe.2023.1183333 Text en Copyright © 2023 Zhang, Luo, Yin, Yin, Zheng and Fu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Zhang, Yingying
Luo, Xi
Yin, Longfei
Yin, Fengwei
Zheng, Weilong
Fu, Yongqian
Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation
title Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation
title_full Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation
title_fullStr Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation
title_full_unstemmed Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation
title_short Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation
title_sort isolation and screening of a chitin deacetylase producing bacillus cereus and its potential for chitosan preparation
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098122/
https://www.ncbi.nlm.nih.gov/pubmed/37064228
http://dx.doi.org/10.3389/fbioe.2023.1183333
work_keys_str_mv AT zhangyingying isolationandscreeningofachitindeacetylaseproducingbacilluscereusanditspotentialforchitosanpreparation
AT luoxi isolationandscreeningofachitindeacetylaseproducingbacilluscereusanditspotentialforchitosanpreparation
AT yinlongfei isolationandscreeningofachitindeacetylaseproducingbacilluscereusanditspotentialforchitosanpreparation
AT yinfengwei isolationandscreeningofachitindeacetylaseproducingbacilluscereusanditspotentialforchitosanpreparation
AT zhengweilong isolationandscreeningofachitindeacetylaseproducingbacilluscereusanditspotentialforchitosanpreparation
AT fuyongqian isolationandscreeningofachitindeacetylaseproducingbacilluscereusanditspotentialforchitosanpreparation