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Dynamics of oral microbiome acquisition in healthy infants: A pilot study

OBJECTIVES: The human oral microbiota is one of the most complex bacterial communities in the human body. However, how newborns initially acquire these bacteria remains largely unknown. In this study, we examined the dynamics of oral microbial communities in healthy infants and investigated the infl...

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Autores principales: Li, Yihong, Saraithong, Prakaimuk, Zhang, Lanxin, Dills, Ashley, Paster, Bruce J., Xiao, Jin, Wu, Tong Tong, Jones, Zachary
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098328/
https://www.ncbi.nlm.nih.gov/pubmed/37065420
http://dx.doi.org/10.3389/froh.2023.1152601
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author Li, Yihong
Saraithong, Prakaimuk
Zhang, Lanxin
Dills, Ashley
Paster, Bruce J.
Xiao, Jin
Wu, Tong Tong
Jones, Zachary
author_facet Li, Yihong
Saraithong, Prakaimuk
Zhang, Lanxin
Dills, Ashley
Paster, Bruce J.
Xiao, Jin
Wu, Tong Tong
Jones, Zachary
author_sort Li, Yihong
collection PubMed
description OBJECTIVES: The human oral microbiota is one of the most complex bacterial communities in the human body. However, how newborns initially acquire these bacteria remains largely unknown. In this study, we examined the dynamics of oral microbial communities in healthy infants and investigated the influence of the maternal oral microbiota on the acquisition of the infant's oral microbiota. We hypothesized that the infant oral microbial diversity increases with age. METHODS: One hundred and sixteen whole-salivary samples were collected from 32 healthy infants and their biological mothers during postpartum and 9- and 15-month well-infant visits. Bacterial genomic DNA was extracted and sequenced by Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) methods. The Shannon index was used to measure the microbial diversity of the infant-mother dyads (alpha diversity). The microbial diversity between the mother-infant dyads (beta-diversity) was calculated using the weighted non-phylogenetic Bray-Curtis distance in QIIME 1.9.1. Core microbiome analysis was performed using MicrobiomeAnalyst software. Linear discriminant analysis coupled with effect size analysis was used to identify differentially abundant features between mother and infant dyads. RESULTS: A total of 6,870,571 16S rRNA reads were generated from paired mother–infant saliva samples. Overall, oral microbial profiles significantly differed between the mother and infant groups (p < 0.001). The diversity of the salivary microbiomes in the infants increased in an age-dependent manner, whereas the core microbiome of the mothers remained relatively stable during the study period. Breastfeeding and gender did not affect the microbial diversity in infants. Moreover, infants had a greater relative abundance of Firmicutes and a lower abundance of Actinobacteria, Bacteroidetes, Fusobacteria, and Proteobacteria than their mothers. The SparCC correlation analysis demonstrated constant changes in infants' oral microbial community network (p < 0.05). CONCLUSIONS: This study provides new evidence that the oral cavities of infants are colonized by a distinct group of bacterial species at birth. The acquisition and diversity of changes in oral microbial composition are dynamic during the first year of an infant's life. Before reaching the second birthday, the composition of the oral microbial community could be more similar to that of their biological mothers.
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spelling pubmed-100983282023-04-14 Dynamics of oral microbiome acquisition in healthy infants: A pilot study Li, Yihong Saraithong, Prakaimuk Zhang, Lanxin Dills, Ashley Paster, Bruce J. Xiao, Jin Wu, Tong Tong Jones, Zachary Front Oral Health Oral Health OBJECTIVES: The human oral microbiota is one of the most complex bacterial communities in the human body. However, how newborns initially acquire these bacteria remains largely unknown. In this study, we examined the dynamics of oral microbial communities in healthy infants and investigated the influence of the maternal oral microbiota on the acquisition of the infant's oral microbiota. We hypothesized that the infant oral microbial diversity increases with age. METHODS: One hundred and sixteen whole-salivary samples were collected from 32 healthy infants and their biological mothers during postpartum and 9- and 15-month well-infant visits. Bacterial genomic DNA was extracted and sequenced by Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) methods. The Shannon index was used to measure the microbial diversity of the infant-mother dyads (alpha diversity). The microbial diversity between the mother-infant dyads (beta-diversity) was calculated using the weighted non-phylogenetic Bray-Curtis distance in QIIME 1.9.1. Core microbiome analysis was performed using MicrobiomeAnalyst software. Linear discriminant analysis coupled with effect size analysis was used to identify differentially abundant features between mother and infant dyads. RESULTS: A total of 6,870,571 16S rRNA reads were generated from paired mother–infant saliva samples. Overall, oral microbial profiles significantly differed between the mother and infant groups (p < 0.001). The diversity of the salivary microbiomes in the infants increased in an age-dependent manner, whereas the core microbiome of the mothers remained relatively stable during the study period. Breastfeeding and gender did not affect the microbial diversity in infants. Moreover, infants had a greater relative abundance of Firmicutes and a lower abundance of Actinobacteria, Bacteroidetes, Fusobacteria, and Proteobacteria than their mothers. The SparCC correlation analysis demonstrated constant changes in infants' oral microbial community network (p < 0.05). CONCLUSIONS: This study provides new evidence that the oral cavities of infants are colonized by a distinct group of bacterial species at birth. The acquisition and diversity of changes in oral microbial composition are dynamic during the first year of an infant's life. Before reaching the second birthday, the composition of the oral microbial community could be more similar to that of their biological mothers. Frontiers Media S.A. 2023-03-30 /pmc/articles/PMC10098328/ /pubmed/37065420 http://dx.doi.org/10.3389/froh.2023.1152601 Text en © 2023 Li, Saraithong, Zhang, Dills, Paster, Xiao, Wu and Jones. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oral Health
Li, Yihong
Saraithong, Prakaimuk
Zhang, Lanxin
Dills, Ashley
Paster, Bruce J.
Xiao, Jin
Wu, Tong Tong
Jones, Zachary
Dynamics of oral microbiome acquisition in healthy infants: A pilot study
title Dynamics of oral microbiome acquisition in healthy infants: A pilot study
title_full Dynamics of oral microbiome acquisition in healthy infants: A pilot study
title_fullStr Dynamics of oral microbiome acquisition in healthy infants: A pilot study
title_full_unstemmed Dynamics of oral microbiome acquisition in healthy infants: A pilot study
title_short Dynamics of oral microbiome acquisition in healthy infants: A pilot study
title_sort dynamics of oral microbiome acquisition in healthy infants: a pilot study
topic Oral Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098328/
https://www.ncbi.nlm.nih.gov/pubmed/37065420
http://dx.doi.org/10.3389/froh.2023.1152601
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