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Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response

Triclocarban (TCC), an emerging organic contaminant (EOC), has become a severe threat to soil microbial communities and ecological security. Here, the TCC-degrading strain Rhodococcus rhodochrous BX2 and DCA-degrading strain Pseudomonas sp. LY-1 (together referred to as TC1) were immobilized on bioc...

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Autores principales: Miao, Lei, Chen, Siyuan, Yang, Hua, Hong, Yaqi, Sun, Liwen, Yang, Jie, Sun, Guanjun, Liu, Yi, Li, Chunyan, Zang, Hailian, Cheng, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098447/
https://www.ncbi.nlm.nih.gov/pubmed/37065135
http://dx.doi.org/10.3389/fmicb.2023.1168902
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author Miao, Lei
Chen, Siyuan
Yang, Hua
Hong, Yaqi
Sun, Liwen
Yang, Jie
Sun, Guanjun
Liu, Yi
Li, Chunyan
Zang, Hailian
Cheng, Yi
author_facet Miao, Lei
Chen, Siyuan
Yang, Hua
Hong, Yaqi
Sun, Liwen
Yang, Jie
Sun, Guanjun
Liu, Yi
Li, Chunyan
Zang, Hailian
Cheng, Yi
author_sort Miao, Lei
collection PubMed
description Triclocarban (TCC), an emerging organic contaminant (EOC), has become a severe threat to soil microbial communities and ecological security. Here, the TCC-degrading strain Rhodococcus rhodochrous BX2 and DCA-degrading strain Pseudomonas sp. LY-1 (together referred to as TC1) were immobilized on biochar to remove TCC and its intermediates in TCC-contaminated soil. High-throughput sequencing was used to investigate the microbial community structure in TCC-contaminated soil. Analysis of co-occurrence networks was used to explore the mutual relationships among soil microbiome members. The results showed that the immobilized TC1 significantly increased the removal efficiency of TCC from 84.7 to 92.7% compared to CK (no TC1 cells on biochar) in 10 mg/L TCC liquid medium. The utilization of immobilized TC1 also significantly accelerated the removal of TCC from contaminated soil. Microbial community analysis revealed the crucial microorganisms and their functional enzymes participating in TCC degradation in soil. Moreover, the internal labor division patterns and connections of TCC-degrading microbes, with a focus on strains BX2 and LY-1, were unraveled by co-occurrence networks analysis. This work provides a promising strategy to facilitate the bioremediation of TCC in soil, which has potential application value for sustainable biobased economies.
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spelling pubmed-100984472023-04-14 Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response Miao, Lei Chen, Siyuan Yang, Hua Hong, Yaqi Sun, Liwen Yang, Jie Sun, Guanjun Liu, Yi Li, Chunyan Zang, Hailian Cheng, Yi Front Microbiol Microbiology Triclocarban (TCC), an emerging organic contaminant (EOC), has become a severe threat to soil microbial communities and ecological security. Here, the TCC-degrading strain Rhodococcus rhodochrous BX2 and DCA-degrading strain Pseudomonas sp. LY-1 (together referred to as TC1) were immobilized on biochar to remove TCC and its intermediates in TCC-contaminated soil. High-throughput sequencing was used to investigate the microbial community structure in TCC-contaminated soil. Analysis of co-occurrence networks was used to explore the mutual relationships among soil microbiome members. The results showed that the immobilized TC1 significantly increased the removal efficiency of TCC from 84.7 to 92.7% compared to CK (no TC1 cells on biochar) in 10 mg/L TCC liquid medium. The utilization of immobilized TC1 also significantly accelerated the removal of TCC from contaminated soil. Microbial community analysis revealed the crucial microorganisms and their functional enzymes participating in TCC degradation in soil. Moreover, the internal labor division patterns and connections of TCC-degrading microbes, with a focus on strains BX2 and LY-1, were unraveled by co-occurrence networks analysis. This work provides a promising strategy to facilitate the bioremediation of TCC in soil, which has potential application value for sustainable biobased economies. Frontiers Media S.A. 2023-03-30 /pmc/articles/PMC10098447/ /pubmed/37065135 http://dx.doi.org/10.3389/fmicb.2023.1168902 Text en Copyright © 2023 Miao, Chen, Yang, Hong, Sun, Yang, Sun, Liu, Li, Zang and Cheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Miao, Lei
Chen, Siyuan
Yang, Hua
Hong, Yaqi
Sun, Liwen
Yang, Jie
Sun, Guanjun
Liu, Yi
Li, Chunyan
Zang, Hailian
Cheng, Yi
Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response
title Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response
title_full Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response
title_fullStr Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response
title_full_unstemmed Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response
title_short Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response
title_sort enhanced bioremediation of triclocarban-contaminated soil by rhodococcus rhodochrous bx2 and pseudomonas sp. ly-1 immobilized on biochar and microbial community response
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098447/
https://www.ncbi.nlm.nih.gov/pubmed/37065135
http://dx.doi.org/10.3389/fmicb.2023.1168902
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