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Design and Modeling of a Device Combining Single-Cell Exposure to a Uniform Electrical Field and Simultaneous Characterization via Bioimpedance Spectroscopy
Previous studies have demonstrated the electropermeabilization of cell membranes exposed to an electric field with moderate intensity (<2 V/cm) and a frequency of <100 MHz. Bioimpedance spectroscopy (BIS) is an electrical characterization technique that can be useful in studying this phenomeno...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098563/ https://www.ncbi.nlm.nih.gov/pubmed/37050519 http://dx.doi.org/10.3390/s23073460 |
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author | Bettenfeld, Rémi Claudel, Julien Kourtiche, Djilali Nadi, Mustapha Schlauder, Cyril |
author_facet | Bettenfeld, Rémi Claudel, Julien Kourtiche, Djilali Nadi, Mustapha Schlauder, Cyril |
author_sort | Bettenfeld, Rémi |
collection | PubMed |
description | Previous studies have demonstrated the electropermeabilization of cell membranes exposed to an electric field with moderate intensity (<2 V/cm) and a frequency of <100 MHz. Bioimpedance spectroscopy (BIS) is an electrical characterization technique that can be useful in studying this phenomenon because it is already used for electroporation. In this paper, we report a device designed to perform BIS on single cells and expose them to an electric field simultaneously. It also allows cells to be monitored by visualization through a transparent exposure electrode. This device is based on a lab-on-a-chip (LOC) with a microfluidic cell-trapping system and microelectrodes for BIS characterization. We present numerical simulations that support the design of the LOC. We also describe the fabrication of the LOC and the first electrical characterization of its measurement bandwidth. This first test, performed on reference medium with a conductivity in the same order than human cells, confirms that the measurement capabilities of our device are suitable for electrical cells characterization. |
format | Online Article Text |
id | pubmed-10098563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100985632023-04-14 Design and Modeling of a Device Combining Single-Cell Exposure to a Uniform Electrical Field and Simultaneous Characterization via Bioimpedance Spectroscopy Bettenfeld, Rémi Claudel, Julien Kourtiche, Djilali Nadi, Mustapha Schlauder, Cyril Sensors (Basel) Article Previous studies have demonstrated the electropermeabilization of cell membranes exposed to an electric field with moderate intensity (<2 V/cm) and a frequency of <100 MHz. Bioimpedance spectroscopy (BIS) is an electrical characterization technique that can be useful in studying this phenomenon because it is already used for electroporation. In this paper, we report a device designed to perform BIS on single cells and expose them to an electric field simultaneously. It also allows cells to be monitored by visualization through a transparent exposure electrode. This device is based on a lab-on-a-chip (LOC) with a microfluidic cell-trapping system and microelectrodes for BIS characterization. We present numerical simulations that support the design of the LOC. We also describe the fabrication of the LOC and the first electrical characterization of its measurement bandwidth. This first test, performed on reference medium with a conductivity in the same order than human cells, confirms that the measurement capabilities of our device are suitable for electrical cells characterization. MDPI 2023-03-25 /pmc/articles/PMC10098563/ /pubmed/37050519 http://dx.doi.org/10.3390/s23073460 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bettenfeld, Rémi Claudel, Julien Kourtiche, Djilali Nadi, Mustapha Schlauder, Cyril Design and Modeling of a Device Combining Single-Cell Exposure to a Uniform Electrical Field and Simultaneous Characterization via Bioimpedance Spectroscopy |
title | Design and Modeling of a Device Combining Single-Cell Exposure to a Uniform Electrical Field and Simultaneous Characterization via Bioimpedance Spectroscopy |
title_full | Design and Modeling of a Device Combining Single-Cell Exposure to a Uniform Electrical Field and Simultaneous Characterization via Bioimpedance Spectroscopy |
title_fullStr | Design and Modeling of a Device Combining Single-Cell Exposure to a Uniform Electrical Field and Simultaneous Characterization via Bioimpedance Spectroscopy |
title_full_unstemmed | Design and Modeling of a Device Combining Single-Cell Exposure to a Uniform Electrical Field and Simultaneous Characterization via Bioimpedance Spectroscopy |
title_short | Design and Modeling of a Device Combining Single-Cell Exposure to a Uniform Electrical Field and Simultaneous Characterization via Bioimpedance Spectroscopy |
title_sort | design and modeling of a device combining single-cell exposure to a uniform electrical field and simultaneous characterization via bioimpedance spectroscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098563/ https://www.ncbi.nlm.nih.gov/pubmed/37050519 http://dx.doi.org/10.3390/s23073460 |
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