Cargando…
Antiviral effect of melatonin on Japanese encephalitis virus infection involves inhibition of neuronal apoptosis and neuroinflammation in SH-SY5Y cells
Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, causes high mortality rates in humans and it is the most clinically important and common cause of viral encephalitis in Asia. To date, there is no specific treatment for JEV infection. Melatonin, a neurotropic hormone, is reported to be...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099015/ https://www.ncbi.nlm.nih.gov/pubmed/37055489 http://dx.doi.org/10.1038/s41598-023-33254-4 |
_version_ | 1785024955449081856 |
---|---|
author | Kitidee, Kuntida Samutpong, Arisara Pakpian, Nattaporn Wisitponchai, Tanchanok Govitrapong, Piyarat Reiter, Russel J. Wongchitrat, Prapimpun |
author_facet | Kitidee, Kuntida Samutpong, Arisara Pakpian, Nattaporn Wisitponchai, Tanchanok Govitrapong, Piyarat Reiter, Russel J. Wongchitrat, Prapimpun |
author_sort | Kitidee, Kuntida |
collection | PubMed |
description | Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, causes high mortality rates in humans and it is the most clinically important and common cause of viral encephalitis in Asia. To date, there is no specific treatment for JEV infection. Melatonin, a neurotropic hormone, is reported to be effective in combating various bacterial and viral infections. However, the effects of melatonin on JEV infection have not yet been studied. The investigation tested the antiviral effects of melatonin against JEV infection and elucidated the possible molecular mechanisms of inhibition. Melatonin inhibited the viral production in JEV-infected SH-SY5Y cells in a time- and dose-dependent manner. Time-of-addition assays demonstrated a potent inhibitory effect of melatonin at the post-entry stage of viral replication. Molecular docking analysis revealed that melatonin negatively affected viral replication by interfering with physiological function and/or enzymatic activity of both JEV nonstructural 3 (NS3) and NS5 protein, suggesting a possible underlying mechanism of JEV replication inhibition. Moreover, treatment with melatonin reduced neuronal apoptosis and inhibited neuroinflammation induced by JEV infection. The present findings reveal a new property of melatonin as a potential molecule for the further development of anti-JEV agents and treatment of JEV infection. |
format | Online Article Text |
id | pubmed-10099015 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-100990152023-04-14 Antiviral effect of melatonin on Japanese encephalitis virus infection involves inhibition of neuronal apoptosis and neuroinflammation in SH-SY5Y cells Kitidee, Kuntida Samutpong, Arisara Pakpian, Nattaporn Wisitponchai, Tanchanok Govitrapong, Piyarat Reiter, Russel J. Wongchitrat, Prapimpun Sci Rep Article Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, causes high mortality rates in humans and it is the most clinically important and common cause of viral encephalitis in Asia. To date, there is no specific treatment for JEV infection. Melatonin, a neurotropic hormone, is reported to be effective in combating various bacterial and viral infections. However, the effects of melatonin on JEV infection have not yet been studied. The investigation tested the antiviral effects of melatonin against JEV infection and elucidated the possible molecular mechanisms of inhibition. Melatonin inhibited the viral production in JEV-infected SH-SY5Y cells in a time- and dose-dependent manner. Time-of-addition assays demonstrated a potent inhibitory effect of melatonin at the post-entry stage of viral replication. Molecular docking analysis revealed that melatonin negatively affected viral replication by interfering with physiological function and/or enzymatic activity of both JEV nonstructural 3 (NS3) and NS5 protein, suggesting a possible underlying mechanism of JEV replication inhibition. Moreover, treatment with melatonin reduced neuronal apoptosis and inhibited neuroinflammation induced by JEV infection. The present findings reveal a new property of melatonin as a potential molecule for the further development of anti-JEV agents and treatment of JEV infection. Nature Publishing Group UK 2023-04-13 /pmc/articles/PMC10099015/ /pubmed/37055489 http://dx.doi.org/10.1038/s41598-023-33254-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kitidee, Kuntida Samutpong, Arisara Pakpian, Nattaporn Wisitponchai, Tanchanok Govitrapong, Piyarat Reiter, Russel J. Wongchitrat, Prapimpun Antiviral effect of melatonin on Japanese encephalitis virus infection involves inhibition of neuronal apoptosis and neuroinflammation in SH-SY5Y cells |
title | Antiviral effect of melatonin on Japanese encephalitis virus infection involves inhibition of neuronal apoptosis and neuroinflammation in SH-SY5Y cells |
title_full | Antiviral effect of melatonin on Japanese encephalitis virus infection involves inhibition of neuronal apoptosis and neuroinflammation in SH-SY5Y cells |
title_fullStr | Antiviral effect of melatonin on Japanese encephalitis virus infection involves inhibition of neuronal apoptosis and neuroinflammation in SH-SY5Y cells |
title_full_unstemmed | Antiviral effect of melatonin on Japanese encephalitis virus infection involves inhibition of neuronal apoptosis and neuroinflammation in SH-SY5Y cells |
title_short | Antiviral effect of melatonin on Japanese encephalitis virus infection involves inhibition of neuronal apoptosis and neuroinflammation in SH-SY5Y cells |
title_sort | antiviral effect of melatonin on japanese encephalitis virus infection involves inhibition of neuronal apoptosis and neuroinflammation in sh-sy5y cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099015/ https://www.ncbi.nlm.nih.gov/pubmed/37055489 http://dx.doi.org/10.1038/s41598-023-33254-4 |
work_keys_str_mv | AT kitideekuntida antiviraleffectofmelatoninonjapaneseencephalitisvirusinfectioninvolvesinhibitionofneuronalapoptosisandneuroinflammationinshsy5ycells AT samutpongarisara antiviraleffectofmelatoninonjapaneseencephalitisvirusinfectioninvolvesinhibitionofneuronalapoptosisandneuroinflammationinshsy5ycells AT pakpiannattaporn antiviraleffectofmelatoninonjapaneseencephalitisvirusinfectioninvolvesinhibitionofneuronalapoptosisandneuroinflammationinshsy5ycells AT wisitponchaitanchanok antiviraleffectofmelatoninonjapaneseencephalitisvirusinfectioninvolvesinhibitionofneuronalapoptosisandneuroinflammationinshsy5ycells AT govitrapongpiyarat antiviraleffectofmelatoninonjapaneseencephalitisvirusinfectioninvolvesinhibitionofneuronalapoptosisandneuroinflammationinshsy5ycells AT reiterrusselj antiviraleffectofmelatoninonjapaneseencephalitisvirusinfectioninvolvesinhibitionofneuronalapoptosisandneuroinflammationinshsy5ycells AT wongchitratprapimpun antiviraleffectofmelatoninonjapaneseencephalitisvirusinfectioninvolvesinhibitionofneuronalapoptosisandneuroinflammationinshsy5ycells |