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A Sequential Electrospinning of a Coaxial and Blending Process for Creating Double-Layer Hybrid Films to Sense Glucose

This study presents a glucose biosensor based on electrospun core–sheath nanofibers. Two types of film were fabricated using different electrospinning procedures. Film F1 was composed solely of core–sheath nanofibers fabricated using a modified coaxial electrospinning process. Film F2 was a double-l...

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Autores principales: Du, Yutong, Yang, Zili, Kang, Shixiong, Yu, Deng-Guang, Chen, Xiren, Shao, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099372/
https://www.ncbi.nlm.nih.gov/pubmed/37050745
http://dx.doi.org/10.3390/s23073685
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author Du, Yutong
Yang, Zili
Kang, Shixiong
Yu, Deng-Guang
Chen, Xiren
Shao, Jun
author_facet Du, Yutong
Yang, Zili
Kang, Shixiong
Yu, Deng-Guang
Chen, Xiren
Shao, Jun
author_sort Du, Yutong
collection PubMed
description This study presents a glucose biosensor based on electrospun core–sheath nanofibers. Two types of film were fabricated using different electrospinning procedures. Film F1 was composed solely of core–sheath nanofibers fabricated using a modified coaxial electrospinning process. Film F2 was a double-layer hybrid film fabricated through a sequential electrospinning and blending process. The bottom layer of F2 comprised core–sheath nanofibers fabricated using a modified process, in which pure polymethacrylate type A (Eudragit L100) was used as the core section and water-soluble lignin (WSL) and phenol were loaded as the sheath section. The top layer of F2 contained glucose oxidase (GOx) and gold nanoparticles, which were distributed throughout the polyvinylpyrrolidone K90 (PVP K90) nanofibers through a single-fluid blending electrospinning process. The study investigated the sequential electrospinning process in detail. The experimental results demonstrated that the F2 hybrid film had a higher degradation efficiency of β-D-glucose than F1, reaching a maximum of over 70% after 12 h within the concentration range of 10–40 mmol/L. The hybrid film F2 is used for colorimetric sensing of β-D-glucose in the range of 1–15 mmol/L. The solution exhibited a color that deepened gradually with an increase in β-D-glucose concentration. Electrospinning is flexible in creating structures for bio-cascade reactions, and the double-layer hybrid film can provide a simple template for developing other sensing nanomaterials.
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spelling pubmed-100993722023-04-14 A Sequential Electrospinning of a Coaxial and Blending Process for Creating Double-Layer Hybrid Films to Sense Glucose Du, Yutong Yang, Zili Kang, Shixiong Yu, Deng-Guang Chen, Xiren Shao, Jun Sensors (Basel) Article This study presents a glucose biosensor based on electrospun core–sheath nanofibers. Two types of film were fabricated using different electrospinning procedures. Film F1 was composed solely of core–sheath nanofibers fabricated using a modified coaxial electrospinning process. Film F2 was a double-layer hybrid film fabricated through a sequential electrospinning and blending process. The bottom layer of F2 comprised core–sheath nanofibers fabricated using a modified process, in which pure polymethacrylate type A (Eudragit L100) was used as the core section and water-soluble lignin (WSL) and phenol were loaded as the sheath section. The top layer of F2 contained glucose oxidase (GOx) and gold nanoparticles, which were distributed throughout the polyvinylpyrrolidone K90 (PVP K90) nanofibers through a single-fluid blending electrospinning process. The study investigated the sequential electrospinning process in detail. The experimental results demonstrated that the F2 hybrid film had a higher degradation efficiency of β-D-glucose than F1, reaching a maximum of over 70% after 12 h within the concentration range of 10–40 mmol/L. The hybrid film F2 is used for colorimetric sensing of β-D-glucose in the range of 1–15 mmol/L. The solution exhibited a color that deepened gradually with an increase in β-D-glucose concentration. Electrospinning is flexible in creating structures for bio-cascade reactions, and the double-layer hybrid film can provide a simple template for developing other sensing nanomaterials. MDPI 2023-04-02 /pmc/articles/PMC10099372/ /pubmed/37050745 http://dx.doi.org/10.3390/s23073685 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Du, Yutong
Yang, Zili
Kang, Shixiong
Yu, Deng-Guang
Chen, Xiren
Shao, Jun
A Sequential Electrospinning of a Coaxial and Blending Process for Creating Double-Layer Hybrid Films to Sense Glucose
title A Sequential Electrospinning of a Coaxial and Blending Process for Creating Double-Layer Hybrid Films to Sense Glucose
title_full A Sequential Electrospinning of a Coaxial and Blending Process for Creating Double-Layer Hybrid Films to Sense Glucose
title_fullStr A Sequential Electrospinning of a Coaxial and Blending Process for Creating Double-Layer Hybrid Films to Sense Glucose
title_full_unstemmed A Sequential Electrospinning of a Coaxial and Blending Process for Creating Double-Layer Hybrid Films to Sense Glucose
title_short A Sequential Electrospinning of a Coaxial and Blending Process for Creating Double-Layer Hybrid Films to Sense Glucose
title_sort sequential electrospinning of a coaxial and blending process for creating double-layer hybrid films to sense glucose
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099372/
https://www.ncbi.nlm.nih.gov/pubmed/37050745
http://dx.doi.org/10.3390/s23073685
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