Genome-wide identification of Aux/IAA and ARF gene families reveal their potential roles in flower opening of Dendrobium officinale

BACKGROUND: The auxin indole-3-acetic acid (IAA) is a vital phytohormone that influences plant growth and development. Our previous work showed that IAA content decreased during flower development in the medicinally important orchid Dendrobium officinale, while Aux/IAA genes were downregulated. Howe...

Descripción completa

Detalles Bibliográficos
Autores principales: Si, Can, Zeng, Danqi, da Silva, Jaime A. Teixeira, Qiu, Shengxiang, Duan, Jun, Bai, Song, He, Chunmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099678/
https://www.ncbi.nlm.nih.gov/pubmed/37055721
http://dx.doi.org/10.1186/s12864-023-09263-y
_version_ 1785025105801248768
author Si, Can
Zeng, Danqi
da Silva, Jaime A. Teixeira
Qiu, Shengxiang
Duan, Jun
Bai, Song
He, Chunmei
author_facet Si, Can
Zeng, Danqi
da Silva, Jaime A. Teixeira
Qiu, Shengxiang
Duan, Jun
Bai, Song
He, Chunmei
author_sort Si, Can
collection PubMed
description BACKGROUND: The auxin indole-3-acetic acid (IAA) is a vital phytohormone that influences plant growth and development. Our previous work showed that IAA content decreased during flower development in the medicinally important orchid Dendrobium officinale, while Aux/IAA genes were downregulated. However, little information about auxin-responsive genes and their roles in D. officinale flower development exists. RESULTS: This study validated 14 DoIAA and 26 DoARF early auxin-responsive genes in the D. officinale genome. A phylogenetic analysis classified the DoIAA genes into two subgroups. An analysis of cis-regulatory elements indicated that they were related by phytohormones and abiotic stresses. Gene expression profiles were tissue-specific. Most DoIAA genes (except for DoIAA7) were sensitive to IAA (10 μmol/L) and were downregulated during flower development. Four DoIAA proteins (DoIAA1, DoIAA6, DoIAA10 and DoIAA13) were mainly localized in the nucleus. A yeast two-hybrid assay showed that these four DoIAA proteins interacted with three DoARF proteins (DoARF2, DoARF17, DoARF23). CONCLUSIONS: The structure and molecular functions of early auxin-responsive genes in D. officinale were investigated. The DoIAA-DoARF interaction may play an important role in flower development via the auxin signaling pathway. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09263-y.
format Online
Article
Text
id pubmed-10099678
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-100996782023-04-14 Genome-wide identification of Aux/IAA and ARF gene families reveal their potential roles in flower opening of Dendrobium officinale Si, Can Zeng, Danqi da Silva, Jaime A. Teixeira Qiu, Shengxiang Duan, Jun Bai, Song He, Chunmei BMC Genomics Research BACKGROUND: The auxin indole-3-acetic acid (IAA) is a vital phytohormone that influences plant growth and development. Our previous work showed that IAA content decreased during flower development in the medicinally important orchid Dendrobium officinale, while Aux/IAA genes were downregulated. However, little information about auxin-responsive genes and their roles in D. officinale flower development exists. RESULTS: This study validated 14 DoIAA and 26 DoARF early auxin-responsive genes in the D. officinale genome. A phylogenetic analysis classified the DoIAA genes into two subgroups. An analysis of cis-regulatory elements indicated that they were related by phytohormones and abiotic stresses. Gene expression profiles were tissue-specific. Most DoIAA genes (except for DoIAA7) were sensitive to IAA (10 μmol/L) and were downregulated during flower development. Four DoIAA proteins (DoIAA1, DoIAA6, DoIAA10 and DoIAA13) were mainly localized in the nucleus. A yeast two-hybrid assay showed that these four DoIAA proteins interacted with three DoARF proteins (DoARF2, DoARF17, DoARF23). CONCLUSIONS: The structure and molecular functions of early auxin-responsive genes in D. officinale were investigated. The DoIAA-DoARF interaction may play an important role in flower development via the auxin signaling pathway. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09263-y. BioMed Central 2023-04-13 /pmc/articles/PMC10099678/ /pubmed/37055721 http://dx.doi.org/10.1186/s12864-023-09263-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Si, Can
Zeng, Danqi
da Silva, Jaime A. Teixeira
Qiu, Shengxiang
Duan, Jun
Bai, Song
He, Chunmei
Genome-wide identification of Aux/IAA and ARF gene families reveal their potential roles in flower opening of Dendrobium officinale
title Genome-wide identification of Aux/IAA and ARF gene families reveal their potential roles in flower opening of Dendrobium officinale
title_full Genome-wide identification of Aux/IAA and ARF gene families reveal their potential roles in flower opening of Dendrobium officinale
title_fullStr Genome-wide identification of Aux/IAA and ARF gene families reveal their potential roles in flower opening of Dendrobium officinale
title_full_unstemmed Genome-wide identification of Aux/IAA and ARF gene families reveal their potential roles in flower opening of Dendrobium officinale
title_short Genome-wide identification of Aux/IAA and ARF gene families reveal their potential roles in flower opening of Dendrobium officinale
title_sort genome-wide identification of aux/iaa and arf gene families reveal their potential roles in flower opening of dendrobium officinale
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099678/
https://www.ncbi.nlm.nih.gov/pubmed/37055721
http://dx.doi.org/10.1186/s12864-023-09263-y
work_keys_str_mv AT sican genomewideidentificationofauxiaaandarfgenefamiliesrevealtheirpotentialrolesinfloweropeningofdendrobiumofficinale
AT zengdanqi genomewideidentificationofauxiaaandarfgenefamiliesrevealtheirpotentialrolesinfloweropeningofdendrobiumofficinale
AT dasilvajaimeateixeira genomewideidentificationofauxiaaandarfgenefamiliesrevealtheirpotentialrolesinfloweropeningofdendrobiumofficinale
AT qiushengxiang genomewideidentificationofauxiaaandarfgenefamiliesrevealtheirpotentialrolesinfloweropeningofdendrobiumofficinale
AT duanjun genomewideidentificationofauxiaaandarfgenefamiliesrevealtheirpotentialrolesinfloweropeningofdendrobiumofficinale
AT baisong genomewideidentificationofauxiaaandarfgenefamiliesrevealtheirpotentialrolesinfloweropeningofdendrobiumofficinale
AT hechunmei genomewideidentificationofauxiaaandarfgenefamiliesrevealtheirpotentialrolesinfloweropeningofdendrobiumofficinale

Ejemplares similares