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Valorization of Adhatoda vasica leaves: Extraction, in vitro analyses and in silico approaches

Adhatoda vasica (also called Vasaka) is a traditional medicinal herb used traditionally for the relief of cough, asthma, nasal congestion, bronchial inflammation, upper respiratory infections, bleeding disorders, skin diseases, leprosy, tuberculosis, diabetes, allergic conditions, rheumatism, tumor,...

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Autores principales: Rudrapal, Mithun, Vallinayagam, Sugumari, Aldosari, Sahar, Khan, Johra, Albadrani, Hind, Al-Shareeda, Alaa, Kamal, Mehnaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099809/
https://www.ncbi.nlm.nih.gov/pubmed/37063312
http://dx.doi.org/10.3389/fnut.2023.1161471
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author Rudrapal, Mithun
Vallinayagam, Sugumari
Aldosari, Sahar
Khan, Johra
Albadrani, Hind
Al-Shareeda, Alaa
Kamal, Mehnaz
author_facet Rudrapal, Mithun
Vallinayagam, Sugumari
Aldosari, Sahar
Khan, Johra
Albadrani, Hind
Al-Shareeda, Alaa
Kamal, Mehnaz
author_sort Rudrapal, Mithun
collection PubMed
description Adhatoda vasica (also called Vasaka) is a traditional medicinal herb used traditionally for the relief of cough, asthma, nasal congestion, bronchial inflammation, upper respiratory infections, bleeding disorders, skin diseases, leprosy, tuberculosis, diabetes, allergic conditions, rheumatism, tumor, and many more diseases. The present study aims to investigate the biological activities of vasicine, a potent alkaloid from A. vasica with different biological/ pharmacological assays and in silico techniques. Vasicine showed antimicrobial activity as evidenced fromthe colony-forming unit assay. It showed antioxidant activity in ABTS scavenging assay (IC(50) = 11.5 μg/ml), ferric reducing power assay (IC(50) = 15 μg/ml), DPPH radical scavenging assay (IC(50) = 18.2 μg/ml), hydroxyl radical scavenging assay (IC(50) = 22 μg/ml), and hydrogen peroxide assay (IC(50) = 27.8 μg/ml). It also showed anti-inflammatory activity in proteinase inhibitory assay (IC(50) = 76 μg/ml), BSA method (IC(50) = 51.7 μg/ml), egg albumin method (IC(50) = 53.2 μg/ml), and lipooxygenase inhibition assay (IC(50) = 76 μg/ml). Vasicine showed antidiabetic activity in α-amylase inhibition assay (IC(50) = 47.6 μg/ml), α-glucosidase inhibition assay (IC(50) = 49.68 μg/ml), and non-enzymatic glycosylation of hemoglobin assay. It showed antiviral activity against HIV-protease (IC(50) = 38.5 μg/ml). Vasicine also showed anticancer activity against lung cancer cells (IC(50) = 46.5 μg/ml) and human fibroblast cells (IC(50) = 82.5 μg/ml). In silico studies revealed that similar to the native ligands, vasicine also showed a low binding energy, i.e., good binding affinity for the active binding sites and interacted with α-amylase (-6.7 kcal/mol), α-glucosidase (-7.6 kcal/mol), cyclooxygenase (-7.4 kcal/mol), epidermal growth factor receptor (-6.4 kcal/mol), lipooxygenase (-6.9 kcal/mol), and HIV-protease (-6.4 kcal/mol). The present study ascertains the potential of vasicine as a bioactive compound isolated from A. vasica having therapeutic usefulness in many human diseases.
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spelling pubmed-100998092023-04-14 Valorization of Adhatoda vasica leaves: Extraction, in vitro analyses and in silico approaches Rudrapal, Mithun Vallinayagam, Sugumari Aldosari, Sahar Khan, Johra Albadrani, Hind Al-Shareeda, Alaa Kamal, Mehnaz Front Nutr Nutrition Adhatoda vasica (also called Vasaka) is a traditional medicinal herb used traditionally for the relief of cough, asthma, nasal congestion, bronchial inflammation, upper respiratory infections, bleeding disorders, skin diseases, leprosy, tuberculosis, diabetes, allergic conditions, rheumatism, tumor, and many more diseases. The present study aims to investigate the biological activities of vasicine, a potent alkaloid from A. vasica with different biological/ pharmacological assays and in silico techniques. Vasicine showed antimicrobial activity as evidenced fromthe colony-forming unit assay. It showed antioxidant activity in ABTS scavenging assay (IC(50) = 11.5 μg/ml), ferric reducing power assay (IC(50) = 15 μg/ml), DPPH radical scavenging assay (IC(50) = 18.2 μg/ml), hydroxyl radical scavenging assay (IC(50) = 22 μg/ml), and hydrogen peroxide assay (IC(50) = 27.8 μg/ml). It also showed anti-inflammatory activity in proteinase inhibitory assay (IC(50) = 76 μg/ml), BSA method (IC(50) = 51.7 μg/ml), egg albumin method (IC(50) = 53.2 μg/ml), and lipooxygenase inhibition assay (IC(50) = 76 μg/ml). Vasicine showed antidiabetic activity in α-amylase inhibition assay (IC(50) = 47.6 μg/ml), α-glucosidase inhibition assay (IC(50) = 49.68 μg/ml), and non-enzymatic glycosylation of hemoglobin assay. It showed antiviral activity against HIV-protease (IC(50) = 38.5 μg/ml). Vasicine also showed anticancer activity against lung cancer cells (IC(50) = 46.5 μg/ml) and human fibroblast cells (IC(50) = 82.5 μg/ml). In silico studies revealed that similar to the native ligands, vasicine also showed a low binding energy, i.e., good binding affinity for the active binding sites and interacted with α-amylase (-6.7 kcal/mol), α-glucosidase (-7.6 kcal/mol), cyclooxygenase (-7.4 kcal/mol), epidermal growth factor receptor (-6.4 kcal/mol), lipooxygenase (-6.9 kcal/mol), and HIV-protease (-6.4 kcal/mol). The present study ascertains the potential of vasicine as a bioactive compound isolated from A. vasica having therapeutic usefulness in many human diseases. Frontiers Media S.A. 2023-03-17 /pmc/articles/PMC10099809/ /pubmed/37063312 http://dx.doi.org/10.3389/fnut.2023.1161471 Text en Copyright © 2023 Rudrapal, Vallinayagam, Aldosari, Khan, Albadrani, Al-Shareeda and Kamal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Rudrapal, Mithun
Vallinayagam, Sugumari
Aldosari, Sahar
Khan, Johra
Albadrani, Hind
Al-Shareeda, Alaa
Kamal, Mehnaz
Valorization of Adhatoda vasica leaves: Extraction, in vitro analyses and in silico approaches
title Valorization of Adhatoda vasica leaves: Extraction, in vitro analyses and in silico approaches
title_full Valorization of Adhatoda vasica leaves: Extraction, in vitro analyses and in silico approaches
title_fullStr Valorization of Adhatoda vasica leaves: Extraction, in vitro analyses and in silico approaches
title_full_unstemmed Valorization of Adhatoda vasica leaves: Extraction, in vitro analyses and in silico approaches
title_short Valorization of Adhatoda vasica leaves: Extraction, in vitro analyses and in silico approaches
title_sort valorization of adhatoda vasica leaves: extraction, in vitro analyses and in silico approaches
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099809/
https://www.ncbi.nlm.nih.gov/pubmed/37063312
http://dx.doi.org/10.3389/fnut.2023.1161471
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