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Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs

BACKGROUND: Endometrial receptivity plays a vital role in the success of embryo implantation. However, the temporal proteomic profile of porcine endometrium during embryo implantation is still unclear. RESULTS: In this study, the abundance of proteins in endometrium on days 9, 10, 11, 12, 13, 14, 15...

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Autores principales: Wang, Yueying, Xue, Songyi, Liu, Qiaorui, Gao, Dengying, Hua, Renwu, Lei, Minggang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099840/
https://www.ncbi.nlm.nih.gov/pubmed/37055767
http://dx.doi.org/10.1186/s12864-023-09278-5
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author Wang, Yueying
Xue, Songyi
Liu, Qiaorui
Gao, Dengying
Hua, Renwu
Lei, Minggang
author_facet Wang, Yueying
Xue, Songyi
Liu, Qiaorui
Gao, Dengying
Hua, Renwu
Lei, Minggang
author_sort Wang, Yueying
collection PubMed
description BACKGROUND: Endometrial receptivity plays a vital role in the success of embryo implantation. However, the temporal proteomic profile of porcine endometrium during embryo implantation is still unclear. RESULTS: In this study, the abundance of proteins in endometrium on days 9, 10, 11, 12, 13, 14, 15 and 18 of pregnancy (D9, 10, 11, 12, 13, 14, 15 and 18) was profiled via iTRAQ technology. The results showed that 25, 55, 103, 91, 100, 120, 149 proteins were up-regulated, and 24, 70, 169, 159, 164, 161, 198 proteins were down-regulated in porcine endometrium on D10, 11, 12, 13, 14, 15 and 18 compared with that on D9, respectively. Among these differentially abundance proteins (DAPs), Multiple Reaction Monitoring (MRM) results indicated that S100A9, S100A12, HRG and IFI6 were differentially abundance in endometrial during embryo implantation period. Bioinformatics analysis showed that the proteins differentially expressed in the 7 comparisons were involved in important processes and pathways related to immunization, endometrial remodeling, which have a vital effect on embryonic implantation. CONCLUSION: Our results reveal that retinol binding protein 4 (RBP4) could regulate the cell proliferation, migration and apoptosis of endometrial epithelial cells and endometrial stromal cells to affect embryo implantation. This research also provides resources for studies of proteins in endometrium during early pregnancy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09278-5.
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spelling pubmed-100998402023-04-14 Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs Wang, Yueying Xue, Songyi Liu, Qiaorui Gao, Dengying Hua, Renwu Lei, Minggang BMC Genomics Research BACKGROUND: Endometrial receptivity plays a vital role in the success of embryo implantation. However, the temporal proteomic profile of porcine endometrium during embryo implantation is still unclear. RESULTS: In this study, the abundance of proteins in endometrium on days 9, 10, 11, 12, 13, 14, 15 and 18 of pregnancy (D9, 10, 11, 12, 13, 14, 15 and 18) was profiled via iTRAQ technology. The results showed that 25, 55, 103, 91, 100, 120, 149 proteins were up-regulated, and 24, 70, 169, 159, 164, 161, 198 proteins were down-regulated in porcine endometrium on D10, 11, 12, 13, 14, 15 and 18 compared with that on D9, respectively. Among these differentially abundance proteins (DAPs), Multiple Reaction Monitoring (MRM) results indicated that S100A9, S100A12, HRG and IFI6 were differentially abundance in endometrial during embryo implantation period. Bioinformatics analysis showed that the proteins differentially expressed in the 7 comparisons were involved in important processes and pathways related to immunization, endometrial remodeling, which have a vital effect on embryonic implantation. CONCLUSION: Our results reveal that retinol binding protein 4 (RBP4) could regulate the cell proliferation, migration and apoptosis of endometrial epithelial cells and endometrial stromal cells to affect embryo implantation. This research also provides resources for studies of proteins in endometrium during early pregnancy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09278-5. BioMed Central 2023-04-13 /pmc/articles/PMC10099840/ /pubmed/37055767 http://dx.doi.org/10.1186/s12864-023-09278-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wang, Yueying
Xue, Songyi
Liu, Qiaorui
Gao, Dengying
Hua, Renwu
Lei, Minggang
Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs
title Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs
title_full Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs
title_fullStr Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs
title_full_unstemmed Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs
title_short Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs
title_sort proteomic profiles and the function of rbp4 in endometrium during embryo implantation phases in pigs
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099840/
https://www.ncbi.nlm.nih.gov/pubmed/37055767
http://dx.doi.org/10.1186/s12864-023-09278-5
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