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Insights into the potential for mutualistic and harmful host–microbe interactions affecting brown alga freshwater acclimation
Microbes can modify their hosts' stress tolerance, thus potentially enhancing their ecological range. An example of such interactions is Ectocarpus subulatus, one of the few freshwater‐tolerant brown algae. This tolerance is partially due to its (un)cultivated microbiome. We investigated this p...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099861/ https://www.ncbi.nlm.nih.gov/pubmed/36326449 http://dx.doi.org/10.1111/mec.16766 |
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author | KleinJan, Hetty Frioux, Clémence Califano, Gianmaria Aite, Méziane Fremy, Enora Karimi, Elham Corre, Erwan Wichard, Thomas Siegel, Anne Boyen, Catherine Dittami, Simon M. |
author_facet | KleinJan, Hetty Frioux, Clémence Califano, Gianmaria Aite, Méziane Fremy, Enora Karimi, Elham Corre, Erwan Wichard, Thomas Siegel, Anne Boyen, Catherine Dittami, Simon M. |
author_sort | KleinJan, Hetty |
collection | PubMed |
description | Microbes can modify their hosts' stress tolerance, thus potentially enhancing their ecological range. An example of such interactions is Ectocarpus subulatus, one of the few freshwater‐tolerant brown algae. This tolerance is partially due to its (un)cultivated microbiome. We investigated this phenomenon by modifying the microbiome of laboratory‐grown E. subulatus using mild antibiotic treatments, which affected its ability to grow in low salinity. Low salinity acclimation of these algal‐bacterial associations was then compared. Salinity significantly impacted bacterial and viral gene expression, albeit in different ways across algal‐bacterial communities. In contrast, gene expression of the host and metabolite profiles were affected almost exclusively in the freshwater‐intolerant algal‐bacterial communities. We found no evidence of bacterial protein production that would directly improve algal stress tolerance. However, vitamin K synthesis is one possible bacterial service missing specifically in freshwater‐intolerant cultures in low salinity. In this condition, we also observed a relative increase in bacterial transcriptomic activity and the induction of microbial genes involved in the biosynthesis of the autoinducer AI‐1, a quorum‐sensing regulator. This could have resulted in dysbiosis by causing a shift in bacterial behaviour in the intolerant algal‐bacterial community. Together, these results provide two promising hypotheses to be examined by future targeted experiments. Although they apply only to the specific study system, they offer an example of how bacteria may impact their host's stress response. |
format | Online Article Text |
id | pubmed-10099861 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100998612023-04-14 Insights into the potential for mutualistic and harmful host–microbe interactions affecting brown alga freshwater acclimation KleinJan, Hetty Frioux, Clémence Califano, Gianmaria Aite, Méziane Fremy, Enora Karimi, Elham Corre, Erwan Wichard, Thomas Siegel, Anne Boyen, Catherine Dittami, Simon M. Mol Ecol ORIGINAL ARTICLES Microbes can modify their hosts' stress tolerance, thus potentially enhancing their ecological range. An example of such interactions is Ectocarpus subulatus, one of the few freshwater‐tolerant brown algae. This tolerance is partially due to its (un)cultivated microbiome. We investigated this phenomenon by modifying the microbiome of laboratory‐grown E. subulatus using mild antibiotic treatments, which affected its ability to grow in low salinity. Low salinity acclimation of these algal‐bacterial associations was then compared. Salinity significantly impacted bacterial and viral gene expression, albeit in different ways across algal‐bacterial communities. In contrast, gene expression of the host and metabolite profiles were affected almost exclusively in the freshwater‐intolerant algal‐bacterial communities. We found no evidence of bacterial protein production that would directly improve algal stress tolerance. However, vitamin K synthesis is one possible bacterial service missing specifically in freshwater‐intolerant cultures in low salinity. In this condition, we also observed a relative increase in bacterial transcriptomic activity and the induction of microbial genes involved in the biosynthesis of the autoinducer AI‐1, a quorum‐sensing regulator. This could have resulted in dysbiosis by causing a shift in bacterial behaviour in the intolerant algal‐bacterial community. Together, these results provide two promising hypotheses to be examined by future targeted experiments. Although they apply only to the specific study system, they offer an example of how bacteria may impact their host's stress response. John Wiley and Sons Inc. 2022-11-23 2023-02 /pmc/articles/PMC10099861/ /pubmed/36326449 http://dx.doi.org/10.1111/mec.16766 Text en © 2022 The Authors. Molecular Ecology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | ORIGINAL ARTICLES KleinJan, Hetty Frioux, Clémence Califano, Gianmaria Aite, Méziane Fremy, Enora Karimi, Elham Corre, Erwan Wichard, Thomas Siegel, Anne Boyen, Catherine Dittami, Simon M. Insights into the potential for mutualistic and harmful host–microbe interactions affecting brown alga freshwater acclimation |
title | Insights into the potential for mutualistic and harmful host–microbe interactions affecting brown alga freshwater acclimation |
title_full | Insights into the potential for mutualistic and harmful host–microbe interactions affecting brown alga freshwater acclimation |
title_fullStr | Insights into the potential for mutualistic and harmful host–microbe interactions affecting brown alga freshwater acclimation |
title_full_unstemmed | Insights into the potential for mutualistic and harmful host–microbe interactions affecting brown alga freshwater acclimation |
title_short | Insights into the potential for mutualistic and harmful host–microbe interactions affecting brown alga freshwater acclimation |
title_sort | insights into the potential for mutualistic and harmful host–microbe interactions affecting brown alga freshwater acclimation |
topic | ORIGINAL ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10099861/ https://www.ncbi.nlm.nih.gov/pubmed/36326449 http://dx.doi.org/10.1111/mec.16766 |
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