Cargando…

Purification Analysis, Intracellular Tracking, and Colocalization of Extracellular Vesicles Using Atomic Force and 3D Single-Molecule Localization Microscopy

[Image: see text] Extracellular vesicles (EVs) play a key role in cell–cell communication and thus have great potential to be utilized as therapeutic agents and diagnostic tools. In this study, we implemented single-molecule microscopy techniques as a toolbox for a comprehensive characterization as...

Descripción completa

Detalles Bibliográficos
Autores principales:	Puthukodan, Sujitha, Hofmann, Martina, Mairhofer, Mario, Janout, Hannah, Schurr, Jonas, Hauser, Fabian, Naderer, Christoph, Preiner, Johannes, Winkler, Stephan, Sivun, Dmitry, Jacak, Jaroslaw
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2023
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10100414/ https://www.ncbi.nlm.nih.gov/pubmed/37002540 http://dx.doi.org/10.1021/acs.analchem.3c00144

Ejemplares similares

CRISPR/Cas9 Genome Editing vs. Over-Expression for Fluorescent Extracellular Vesicle-Labeling: A Quantitative Analysis
por: Strohmeier, Karin, et al.
Publicado: (2021)

Statistical analysis of 3D localisation microscopy images for quantification of membrane protein distributions in a platelet clot model
por: Mayr, Sandra, et al.
Publicado: (2020)

Low-Fluorescence Starter for Optical 3D Lithography of Sub-40 nm Structures
por: Gvindzhiliia, Georgii, et al.
Publicado: (2023)

Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching
por: Murtezi, Eljesa, et al.
Publicado: (2018)

Localization Microscopy of Actin Cytoskeleton in Human Platelets
por: Mayr, Sandra, et al.
Publicado: (2018)

Label‐free characterization of an extracellular vesicle‐based therapeutic
por: Priglinger, Eleni, et al.
Publicado: (2021)

Dual Channel Microfluidics for Mimicking the Blood–Brain Barrier
por: Buchroithner, Boris, et al.
Publicado: (2021)

Bio-inspired microneedle design for efficient drug/vaccine coating
por: Plamadeala, Cristina, et al.
Publicado: (2019)

Real-time 3D single-molecule localization microscopy analysis using lookup tables
por: Hauser, Fabian, et al.
Publicado: (2021)

An Improved Transwell Design for Microelectrode Ion-Flux Measurements
por: Buchroithner, Boris, et al.
Publicado: (2021)

Virtual reality assisted microscopy data visualization and colocalization analysis
por: Theart, Rensu P., et al.
Publicado: (2017)

Intracellular colocalization of variant surface glycoprotein and transferrin-gold in Trypanosoma brucei
Publicado: (1988)

Single-vesicle intensity and colocalization fluorescence microscopy to study lipid vesicle fusion, fission, and lipid exchange
por: Andersson, Alexandra, et al.
Publicado: (2022)

Novel approach for accurate tissue-based protein colocalization and proximity microscopy
por: Lutz, Mirjam I., et al.
Publicado: (2017)

Analysis of conditional colocalization relationships and hierarchies in three-color microscopy images
por: Vega-Lugo, Jesus, et al.
Publicado: (2022)

Bridging the gap between qualitative and quantitative colocalization results in fluorescence microscopy studies
por: Zinchuk, Vadim, et al.
Publicado: (2013)

New image colocalization coefficient for fluorescence microscopy to quantify (bio-)molecular interactions
por: Herce, HD, et al.
Publicado: (2013)

A tessellation-based colocalization analysis approach for single-molecule localization microscopy
por: Levet, Florian, et al.
Publicado: (2019)

Stimulated Emission Depletion Lithography with Mercapto-Functional Polymers
por: Buchegger, Bianca, et al.
Publicado: (2016)

ColocQuiaL: a QTL-GWAS colocalization pipeline
por: Chen, Brian Y, et al.
Publicado: (2022)

Colocalization coefficients evaluating the distribution of molecular targets in microscopy methods based on pointed patterns
por: Pastorek, Lukáš, et al.
Publicado: (2016)

Purification and preparation of Rhodobacter sphaeroides reaction centers for photocurrent measurements and atomic force microscopy characterization
por: Jun, Daniel, et al.
Publicado: (2021)

Intracellular Colocalization of Influenza Viral RNA and Rab11A Is Dependent upon Microtubule Filaments
por: Nturibi, Eric, et al.
Publicado: (2017)

Fluorescence colocalization microscopy analysis can be improved by combining object‐recognition with pixel‐intensity‐correlation
por: Moser, Bernhard, et al.
Publicado: (2016)

A density-based enrichment measure for assessing colocalization in single-molecule localization microscopy data
por: Ejdrup, Aske L., et al.
Publicado: (2022)

Determination of oligomeric states of proteins via dual-color colocalization with single molecule localization microscopy
por: Tan, Hua Leonhard, et al.
Publicado: (2022)

EzColocalization: An ImageJ plugin for visualizing and measuring colocalization in cells and organisms
por: Stauffer, Weston, et al.
Publicado: (2018)

Colocalization Analysis of Lipo-Deoxyribozyme Consisting of DNA and Protic Catalysts in a Vesicle-Based Protocellular Membrane Investigated by Confocal Microscopy
por: Hirata, Yuiko, et al.
Publicado: (2021)

Colocalization of synapsin and actin during synaptic vesicle recycling
por: Bloom, Ona, et al.
Publicado: (2003)

Colocalization of endogenous TNF with a functional intracellular splice form of human TNF receptor type 2
por: Scherübl, Christoph, et al.
Publicado: (2005)

3D multiphoton lithography using biocompatible polymers with specific mechanical properties
por: Buchroithner, Boris, et al.
Publicado: (2020)

Coloc-stats: a unified web interface to perform colocalization analysis of genomic features
por: Simovski, Boris, et al.
Publicado: (2018)

A Cryosectioning Technique for the Observation of Intracellular Structures and Immunocytochemistry of Tissues in Atomic Force Microscopy (AFM)
por: Usukura, Eiji, et al.
Publicado: (2017)

Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes
por: Ichikawa, Takehiko, et al.
Publicado: (2023)

Quantitative Colocalization Analysis of Multicolor Confocal Immunofluorescence Microscopy Images: Pushing Pixels to Explore Biological Phenomena
por: Zinchuk, Vadim, et al.
Publicado: (2007)

Dual-color Colocalization in Single-molecule Localization Microscopy to Determine the Oligomeric State of Proteins in the Plasma Membrane
por: Tan, Hua Leonhard, et al.
Publicado: (2023)

Frequency domain photoacoustic and fluorescence microscopy
por: Langer, Gregor, et al.
Publicado: (2016)

Dimerisation of the Yeast K(+) Translocation Protein Trk1 Depends on the K(+) Concentration
por: Kulik, Natalia, et al.
Publicado: (2022)

s-cyclophilin is retained intracellularly via a unique COOH-terminal sequence and colocalizes with the calcium storage protein calreticulin
Publicado: (1992)

epiCOLOC: Integrating Large-Scale and Context-Dependent Epigenomics Features for Comprehensive Colocalization Analysis
por: Zhou, Yao, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_k322n5n10qpuk78fo32mtkru8v