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Glial progenitor cells of the adult human white and grey matter are contextually distinct
Genomic analyses have revealed heterogeneity among glial progenitor cells (GPCs), but the compartment selectivity of human GPCs (hGPCs) is unclear. Here, we asked if GPCs of human grey and white brain matter are distinct in their architecture and associated gene expression. RNA profiling of NG2‐defi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10100527/ https://www.ncbi.nlm.nih.gov/pubmed/36334067 http://dx.doi.org/10.1002/glia.24291 |
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author | Osorio, Maria Joana Mariani, John N. Zou, Lisa Schanz, Steven J. Heffernan, Kate Cornwell, Adam Goldman, Steven A. |
author_facet | Osorio, Maria Joana Mariani, John N. Zou, Lisa Schanz, Steven J. Heffernan, Kate Cornwell, Adam Goldman, Steven A. |
author_sort | Osorio, Maria Joana |
collection | PubMed |
description | Genomic analyses have revealed heterogeneity among glial progenitor cells (GPCs), but the compartment selectivity of human GPCs (hGPCs) is unclear. Here, we asked if GPCs of human grey and white brain matter are distinct in their architecture and associated gene expression. RNA profiling of NG2‐defined hGPCs derived from adult human neocortex and white matter differed in their expression of genes involved in Wnt, NOTCH, BMP and TGFβ signaling, suggesting compartment‐selective biases in fate and self‐renewal. White matter hGPCs over‐expressed the BMP antagonists BAMBI and CHRDL1, suggesting their tonic suppression of astrocytic fate relative to cortical hGPCs, whose relative enrichment of cytoskeletal genes presaged their greater morphological complexity. In human glial chimeric mice, cortical hGPCs assumed larger and more complex morphologies than white matter hGPCs, and both were more complex than their mouse counterparts. These findings suggest that human grey and white matter GPCs comprise context‐specific pools with distinct functional biases. |
format | Online Article Text |
id | pubmed-10100527 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101005272023-04-14 Glial progenitor cells of the adult human white and grey matter are contextually distinct Osorio, Maria Joana Mariani, John N. Zou, Lisa Schanz, Steven J. Heffernan, Kate Cornwell, Adam Goldman, Steven A. Glia Research Articles Genomic analyses have revealed heterogeneity among glial progenitor cells (GPCs), but the compartment selectivity of human GPCs (hGPCs) is unclear. Here, we asked if GPCs of human grey and white brain matter are distinct in their architecture and associated gene expression. RNA profiling of NG2‐defined hGPCs derived from adult human neocortex and white matter differed in their expression of genes involved in Wnt, NOTCH, BMP and TGFβ signaling, suggesting compartment‐selective biases in fate and self‐renewal. White matter hGPCs over‐expressed the BMP antagonists BAMBI and CHRDL1, suggesting their tonic suppression of astrocytic fate relative to cortical hGPCs, whose relative enrichment of cytoskeletal genes presaged their greater morphological complexity. In human glial chimeric mice, cortical hGPCs assumed larger and more complex morphologies than white matter hGPCs, and both were more complex than their mouse counterparts. These findings suggest that human grey and white matter GPCs comprise context‐specific pools with distinct functional biases. John Wiley & Sons, Inc. 2022-11-05 2023-03 /pmc/articles/PMC10100527/ /pubmed/36334067 http://dx.doi.org/10.1002/glia.24291 Text en © 2022 The Authors. GLIA published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Osorio, Maria Joana Mariani, John N. Zou, Lisa Schanz, Steven J. Heffernan, Kate Cornwell, Adam Goldman, Steven A. Glial progenitor cells of the adult human white and grey matter are contextually distinct |
title | Glial progenitor cells of the adult human white and grey matter are contextually distinct |
title_full | Glial progenitor cells of the adult human white and grey matter are contextually distinct |
title_fullStr | Glial progenitor cells of the adult human white and grey matter are contextually distinct |
title_full_unstemmed | Glial progenitor cells of the adult human white and grey matter are contextually distinct |
title_short | Glial progenitor cells of the adult human white and grey matter are contextually distinct |
title_sort | glial progenitor cells of the adult human white and grey matter are contextually distinct |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10100527/ https://www.ncbi.nlm.nih.gov/pubmed/36334067 http://dx.doi.org/10.1002/glia.24291 |
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