Cargando…

Validation of a Reporter Cell Line for Flavivirus Inhibition Assays

Here, we report the validation of a new reporter cell line, Hec1a-IFNB-Luc, for use in inhibition studies of various flaviviruses relevant to human pathology. The reporter system allows the detection of viral replication after luciferase gene activation driven by an interferon beta (IFN-β) promoter....

Descripción completa

Detalles Bibliográficos
Autores principales:	Rezende, Tatiana M. T., Macera, Gabriella, Heyndrickx, Leo, Michiels, Johan, Coppens, Sandra, Thibaut, Hendrik Jan, Dallmeier, Kai, Van Esbroeck, Marjan, Neyts, Johan, Ariën, Kevin K., Bartholomeeusen, Koen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2023
Materias:	Methods and Protocols
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10100686/ https://www.ncbi.nlm.nih.gov/pubmed/36786659 http://dx.doi.org/10.1128/spectrum.05027-22

Ejemplares similares

Three doses of BNT162b2 vaccine confer neutralising antibody capacity against the SARS-CoV-2 Omicron variant
por: Ariën, Kevin K., et al.
Publicado: (2022)

A High-Throughput Yellow Fever Neutralization Assay
por: Rasulova, Madina, et al.
Publicado: (2022)

Dynamics of the Magnitude, Breadth and Depth of the Antibody Response at Epitope Level Following Dengue Infection
por: Falconi-Agapito, Francesca, et al.
Publicado: (2021)

Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay
por: Mariën, Joachim, et al.
Publicado: (2021)

Chikungunya Virus’ High Genomic Plasticity Enables Rapid Adaptation to Restrictive A549 Cells
por: De Caluwé, Lien, et al.
Publicado: (2022)

Evaluation of a surrogate virus neutralization test for high-throughput serosurveillance of SARS-CoV-2
por: Mariën, Joachim, et al.
Publicado: (2021)

MiniCD4 protein resistance mutations affect binding to the HIV-1 gp120 CD4 binding site and decrease entry efficiency
por: Grupping, Katrijn, et al.
Publicado: (2012)

Simple and inexpensive three-step rapid amplification of cDNA 5′ ends using 5′ phosphorylated primers
por: Dallmeier, Kai, et al.
Publicado: (2013)

Zika and Other Emerging Viruses: Aiming at the Right Target
por: Dallmeier, Kai, et al.
Publicado: (2016)

Retrospective detection of asymptomatic monkeypox virus infections among male sexual health clinic attendees in Belgium
por: De Baetselier, Irith, et al.
Publicado: (2022)

Establishment and Application of Heminested RT-PCR Assay for Detection of Mosquito-Borne Flavivirus — Guizhou Province, China, 2018
por: Xue, Zhijing, et al.
Publicado: (2021)

Magnetic bead purification of enveloped alphavirus and flavivirus
por: Galasso, Bianca, et al.
Publicado: (2021)

Chikungunya virus infection in Aruba: Diagnosis, clinical features and predictors of post-chikungunya chronic polyarthralgia
por: Huits, Ralph, et al.
Publicado: (2018)

nsP4 Is a Major Determinant of Alphavirus Replicase Activity and Template Selectivity
por: Lello, Laura Sandra, et al.
Publicado: (2021)

Flaviviral NS4b, chameleon and jack‐in‐the‐box roles in viral replication and pathogenesis, and a molecular target for antiviral intervention
por: Zmurko, Joanna, et al.
Publicado: (2015)

COVID-19 and the intensive care unit: vaccines to the rescue
por: Dallmeier, Kai, et al.
Publicado: (2021)

Autochthonous Cases of Tick-Borne Encephalitis, Belgium, 2020
por: Stoefs, Anke, et al.
Publicado: (2021)

Limited evolution of the yellow fever virus 17d in a mouse infection model
por: Kum, Dieudonné Buh, et al.
Publicado: (2019)

The CD147 Protein Complex Is Involved in Entry of Chikungunya Virus and Related Alphaviruses in Human Cells
por: De Caluwé, Lien, et al.
Publicado: (2021)

A dual-antigen self-amplifying RNA SARS-CoV-2 vaccine induces potent humoral and cellular immune responses and protects against SARS-CoV-2 variants through T cell-mediated immunity
por: McCafferty, Sean, et al.
Publicado: (2022)

Broad-spectrum monoclonal antibodies against chikungunya virus structural proteins: Promising candidates for antibody-based rapid diagnostic test development
por: Tuekprakhon, Aekkachai, et al.
Publicado: (2018)

Severe mpox (formerly monkeypox) disease in five patients after recent vaccination with MVA-BN vaccine, Belgium, July to October 2022
por: Berens-Riha, Nicole, et al.
Publicado: (2022)

A novel therapeutic HBV vaccine candidate induces strong polyfunctional cytotoxic T cell responses in mice
por: Boudewijns, Robbert, et al.
Publicado: (2021)

Comparing immunogenicity and protective efficacy of the yellow fever 17D vaccine in mice
por: Ma, Ji, et al.
Publicado: (2021)

YF17D-vectored Ebola vaccine candidate protects mice against lethal surrogate Ebola and yellow fever virus challenge
por: Lemmens, Viktor, et al.
Publicado: (2023)

An Analogue of the Antibiotic Teicoplanin Prevents Flavivirus Entry In Vitro
por: De Burghgraeve, Tine, et al.
Publicado: (2012)

Zika Virus IgG in Infants with Microcephaly, Guinea-Bissau, 2016
por: Rosenstierne, Maiken Worsøe, et al.
Publicado: (2018)

Alternative sampling specimens for the molecular detection of mpox (formerly monkeypox) virus
por: Coppens, Jasmine, et al.
Publicado: (2023)

Cross-utilisation of template RNAs by alphavirus replicases
por: Lello, Laura Sandra, et al.
Publicado: (2020)

Persistent morbidity in Clade IIb mpox patients: interim results of a long-term follow-up study, Belgium, June to November 2022
por: Berens-Riha, Nicole, et al.
Publicado: (2023)

Insulin secretion assays in an engineered MIN6 cell line
por: Yang, Liu, et al.
Publicado: (2023)

Complete Genome Sequence of a Rat Hepatitis E Virus Strain Isolated in the United States
por: Debing, Yannick, et al.
Publicado: (2014)

A rat model for hepatitis E virus
por: Debing, Yannick, et al.
Publicado: (2016)

MLGA—a rapid and cost-efficient assay for gene copy-number analysis
por: Isaksson, Magnus, et al.
Publicado: (2007)

A loop-mediated isothermal amplification (LAMP) assay for rapid identification of Ceratitis capitata and related species
por: Dermauw, Wannes, et al.
Publicado: (2022)

Zika virus in semen: a prospective cohort study of symptomatic travellers returning to Belgium
por: Huits, Ralph, et al.
Publicado: (2017)

Detection of mpox virus in ambient air in a sexual health clinic
por: Raymenants, Joren, et al.
Publicado: (2023)

Flaviviruses
Publicado: (2005)

Development of a robust and convenient dual-reporter high-throughput screening assay for SARS-CoV-2 antiviral drug discovery
por: Chiu, Winston, et al.
Publicado: (2023)

A chimeric yellow fever-Zika virus vaccine candidate fully protects against yellow fever virus infection in mice
por: Kum, Dieudonné Buh, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_h6e99blt1baj1a3ee2jisdtdd1