Cargando…

Probiotic Potential and Safety Assessment of Type Strains of Weissella and Periweissella Species

Although numerous strains belonging to the Weissella genus have been described in the last decades for their probiotic and biotechnological potential, others are known to be opportunistic pathogens of humans and animals. Here, we investigated the probiotic potential of two Weissella and four Periwei...

Descripción completa

Detalles Bibliográficos
Autores principales: Fanelli, Francesca, Montemurro, Marco, Verni, Michela, Garbetta, Antonella, Bavaro, Anna Rita, Chieffi, Daniele, Cho, Gyu-Sung, Franz, Charles M. A. P., Rizzello, Carlo Giuseppe, Fusco, Vincenzina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10100829/
https://www.ncbi.nlm.nih.gov/pubmed/36847557
http://dx.doi.org/10.1128/spectrum.03047-22
_version_ 1785025366678568960
author Fanelli, Francesca
Montemurro, Marco
Verni, Michela
Garbetta, Antonella
Bavaro, Anna Rita
Chieffi, Daniele
Cho, Gyu-Sung
Franz, Charles M. A. P.
Rizzello, Carlo Giuseppe
Fusco, Vincenzina
author_facet Fanelli, Francesca
Montemurro, Marco
Verni, Michela
Garbetta, Antonella
Bavaro, Anna Rita
Chieffi, Daniele
Cho, Gyu-Sung
Franz, Charles M. A. P.
Rizzello, Carlo Giuseppe
Fusco, Vincenzina
author_sort Fanelli, Francesca
collection PubMed
description Although numerous strains belonging to the Weissella genus have been described in the last decades for their probiotic and biotechnological potential, others are known to be opportunistic pathogens of humans and animals. Here, we investigated the probiotic potential of two Weissella and four Periweissella type strains belonging to the species Weissella diestrammenae, Weissella uvarum, Periweissella beninensis, Periweissella fabalis, Periweissella fabaria, and Periweissella ghanensis by genomic and phenotypic analyses, and performed a safety assessment of these strains. Based on the results of the survival to simulated gastrointestinal transit, autoaggregation and hydrophobicity characteristics, as well as adhesion to Caco-2 cells, we showed that the P. beninensis, P. fabalis, P. fabaria, P. ghanensis, and W. uvarum type strains exhibited a high probiotic potential. The safety assessment, based on the genomic analysis, performed by searching for virulence and antibiotic resistance genes, as well as on the phenotypic evaluation, by testing hemolytic activity and antibiotic susceptibility, allowed us to identify the P. beninensis type strain as a safe potential probiotic microorganism. IMPORTANCE A comprehensive analysis of safety and functional features of six Weissella and Periweissella type strains was performed. Our data demonstrated the probiotic potential of these species, indicating the P. beninensis type strain as the best candidate based on its potential probiotic features and the safety assessment. The presence of different antimicrobial resistance profiles in the analyzed strains highlighted the need to establish cutoff values to perform a standardized safety evaluation of these species, which, in our opinion, should be mandatory on a strain-specific basis.
format Online
Article
Text
id pubmed-10100829
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-101008292023-04-14 Probiotic Potential and Safety Assessment of Type Strains of Weissella and Periweissella Species Fanelli, Francesca Montemurro, Marco Verni, Michela Garbetta, Antonella Bavaro, Anna Rita Chieffi, Daniele Cho, Gyu-Sung Franz, Charles M. A. P. Rizzello, Carlo Giuseppe Fusco, Vincenzina Microbiol Spectr Research Article Although numerous strains belonging to the Weissella genus have been described in the last decades for their probiotic and biotechnological potential, others are known to be opportunistic pathogens of humans and animals. Here, we investigated the probiotic potential of two Weissella and four Periweissella type strains belonging to the species Weissella diestrammenae, Weissella uvarum, Periweissella beninensis, Periweissella fabalis, Periweissella fabaria, and Periweissella ghanensis by genomic and phenotypic analyses, and performed a safety assessment of these strains. Based on the results of the survival to simulated gastrointestinal transit, autoaggregation and hydrophobicity characteristics, as well as adhesion to Caco-2 cells, we showed that the P. beninensis, P. fabalis, P. fabaria, P. ghanensis, and W. uvarum type strains exhibited a high probiotic potential. The safety assessment, based on the genomic analysis, performed by searching for virulence and antibiotic resistance genes, as well as on the phenotypic evaluation, by testing hemolytic activity and antibiotic susceptibility, allowed us to identify the P. beninensis type strain as a safe potential probiotic microorganism. IMPORTANCE A comprehensive analysis of safety and functional features of six Weissella and Periweissella type strains was performed. Our data demonstrated the probiotic potential of these species, indicating the P. beninensis type strain as the best candidate based on its potential probiotic features and the safety assessment. The presence of different antimicrobial resistance profiles in the analyzed strains highlighted the need to establish cutoff values to perform a standardized safety evaluation of these species, which, in our opinion, should be mandatory on a strain-specific basis. American Society for Microbiology 2023-02-27 /pmc/articles/PMC10100829/ /pubmed/36847557 http://dx.doi.org/10.1128/spectrum.03047-22 Text en Copyright © 2023 Fanelli et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Fanelli, Francesca
Montemurro, Marco
Verni, Michela
Garbetta, Antonella
Bavaro, Anna Rita
Chieffi, Daniele
Cho, Gyu-Sung
Franz, Charles M. A. P.
Rizzello, Carlo Giuseppe
Fusco, Vincenzina
Probiotic Potential and Safety Assessment of Type Strains of Weissella and Periweissella Species
title Probiotic Potential and Safety Assessment of Type Strains of Weissella and Periweissella Species
title_full Probiotic Potential and Safety Assessment of Type Strains of Weissella and Periweissella Species
title_fullStr Probiotic Potential and Safety Assessment of Type Strains of Weissella and Periweissella Species
title_full_unstemmed Probiotic Potential and Safety Assessment of Type Strains of Weissella and Periweissella Species
title_short Probiotic Potential and Safety Assessment of Type Strains of Weissella and Periweissella Species
title_sort probiotic potential and safety assessment of type strains of weissella and periweissella species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10100829/
https://www.ncbi.nlm.nih.gov/pubmed/36847557
http://dx.doi.org/10.1128/spectrum.03047-22
work_keys_str_mv AT fanellifrancesca probioticpotentialandsafetyassessmentoftypestrainsofweissellaandperiweissellaspecies
AT montemurromarco probioticpotentialandsafetyassessmentoftypestrainsofweissellaandperiweissellaspecies
AT vernimichela probioticpotentialandsafetyassessmentoftypestrainsofweissellaandperiweissellaspecies
AT garbettaantonella probioticpotentialandsafetyassessmentoftypestrainsofweissellaandperiweissellaspecies
AT bavaroannarita probioticpotentialandsafetyassessmentoftypestrainsofweissellaandperiweissellaspecies
AT chieffidaniele probioticpotentialandsafetyassessmentoftypestrainsofweissellaandperiweissellaspecies
AT chogyusung probioticpotentialandsafetyassessmentoftypestrainsofweissellaandperiweissellaspecies
AT franzcharlesmap probioticpotentialandsafetyassessmentoftypestrainsofweissellaandperiweissellaspecies
AT rizzellocarlogiuseppe probioticpotentialandsafetyassessmentoftypestrainsofweissellaandperiweissellaspecies
AT fuscovincenzina probioticpotentialandsafetyassessmentoftypestrainsofweissellaandperiweissellaspecies