Cargando…

Comparative Evaluation of Standard RT-PCR Assays and Commercial Real-Time RT-PCR Kits for Detection of Lassa Virus

Lassa virus (LASV) is a causative agent of hemorrhagic fever epidemic in West Africa. In recent years, it has been transmitted several times to North America, Europe, and Asia. Standard reverse transcription (RT)-PCR and real-time RT-PCR are extensively used for early detection of LASV. However, the...

Descripción completa

Detalles Bibliográficos
Autores principales:	Luo, Xue-Lian, Zhang, Xiu-Dan, Li, Bei-Jie, Qin, Tian, Cao, Zhi-Jie, Fan, Qian-Jin, Yang, Jing, Jin, Dong, Lu, Shan, Zheng, Ya-Yun, Xu, Xue-Fang, Pu, Ji, Xu, Jianguo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10100986/ https://www.ncbi.nlm.nih.gov/pubmed/36976009 http://dx.doi.org/10.1128/spectrum.05011-22

Ejemplares similares

Comparison of seven commercial RT-PCR diagnostic kits for COVID-19
por: van Kasteren, Puck B., et al.
Publicado: (2020)

Comparison of four commercial RT‐PCR diagnostic kits for COVID‐19 in China
por: Shen, Lingyu, et al.
Publicado: (2020)

Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR
por: Pagliarulo, Vincenzo, et al.
Publicado: (2004)

Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus
por: Dedkov, Vladimir G., et al.
Publicado: (2019)

Evaluation of commercial RT-PCR kits for pooled clinical specimens in COVID-19 patients.
por: Arya, Akshay Kumar, et al.
Publicado: (2021)

Clinical Evaluation of Three Commercial RT-PCR Kits for Routine COVID-19 Diagnosis
por: Juthi, Rifat Tasnim, et al.
Publicado: (2022)

Performance verification of five commercial RT-qPCR diagnostic kits for SARS-CoV-2
por: Yang, Mei, et al.
Publicado: (2022)

Evaluation of four commercial real-time RT-PCR kits for the detection of dengue viruses in clinical samples
por: Najioullah, Fatiha, et al.
Publicado: (2014)

Diagnostic evaluation of qRT-PCR-based kit and dPCR-based kit for COVID-19
por: Lee, Cherl-Joon, et al.
Publicado: (2021)

Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection
por: TAKANO, Tomomi, et al.
Publicado: (2021)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

An Endogenous Murine Leukemia Viral Genome Contaminant in a Commercial RT-PCR Kit is Amplified Using Standard Primers for XMRV
por: Sato, Eiji, et al.
Publicado: (2010)

Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome
por: Nunes, Bruno Tardelli Diniz, et al.
Publicado: (2019)

A Real-Time RT-PCR Assay for Genotyping of Rotavirus
por: Mousavi-Nasab, Seyed Dawood, et al.
Publicado: (2020)

Comparative analysis of the diagnostic performance of five commercial COVID-19 qRT PCR kits used in India
por: Singh, J., et al.
Publicado: (2021)

Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples
por: Yu, Xuelian, et al.
Publicado: (2014)

The Development and Application of the Two Real-Time RT-PCR Assays to Detect the Pathogen of HFMD
por: Cui, Aili, et al.
Publicado: (2013)

Laboratory verification of an RT‐PCR assay for SARS‐CoV‐2
por: Wu, Yingping, et al.
Publicado: (2020)

A RT-PCR assay for the detection of coronaviruses from four genera
por: Xiu, Leshan, et al.
Publicado: (2020)

Development of a real-time TaqMan(®) RT-PCR assay for the detection of infectious bronchitis virus in chickens, and comparison of RT-PCR and virus isolation
por: Meir, Rosie, et al.
Publicado: (2010)

Comparison of three commercial RT-PCR systems for the detection of respiratory viruses
por: Butt, S.A., et al.
Publicado: (2014)

Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay
por: Ma, Lei, et al.
Publicado: (2018)

Development of a universal RT-PCR assay for grapevine vitiviruses
por: Diaz-Lara, Alfredo, et al.
Publicado: (2020)

An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes
por: Sun, Junfeng, et al.
Publicado: (2015)

Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses
por: Lu, Huanhuan, et al.
Publicado: (2021)

COVID-19 Real-Time RT-PCR: Does Positivity on Follow-up RT-PCR Always Imply Infectivity?
por: Suri, Tejas, et al.
Publicado: (2020)

Improving the standardization of mRNA measurement by RT-qPCR
por: Sanders, Rebecca, et al.
Publicado: (2018)

Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
por: Piqueur, Marian AC, et al.
Publicado: (2009)

Detection of influenza C virus by a real‐time RT‐PCR assay
por: Pabbaraju, Kanti, et al.
Publicado: (2013)

Detection of enteroviruses and parechoviruses by a multiplex real-time RT-PCR assay
por: Pabbaraju, Kanti, et al.
Publicado: (2015)

Chest CT of COVID-19 in patients with a negative first RT-PCR test: Comparison with patients with a positive first RT-PCR test
por: Chen, Zu-Hua, et al.
Publicado: (2020)

Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR)
por: Jeong, Jae-Kyo, et al.
Publicado: (2014)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
por: Park, Changwoo, et al.
Publicado: (2023)

Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
por: Jalal, Sehrish, et al.
Publicado: (2021)

Evaluation of process limit of detection and quantification variation of SARS-CoV-2 RT-qPCR and RT-dPCR assays for wastewater surveillance
por: Ahmed, Warish, et al.
Publicado: (2022)

RT-PCR negative COVID-19
por: Parmar, Heta, et al.
Publicado: (2022)

A RT-PCR assay for the detection of encephalomycarditis virus infections in pigs
por: Pérez, Lester J., et al.
Publicado: (2009)

Hepatitis A virus subgenotyping based on RT-qPCR assays
por: Coudray-Meunier, Coralie, et al.
Publicado: (2014)

A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses
por: Vijgen, Leen, et al.
Publicado: (2007)

Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis
por: Soma, Takehisa
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_pul251nkqdoliggpduck046ups