Cargando…

Novel In Situ Hybridization Assay for Chromogenic Single-Molecule Detection of Human Papillomavirus E6/E7 mRNA

RNA plays a vital role in the physiological and pathological processes of cells and tissues. However, RNA in situ hybridization applications in clinical diagnostics are still limited to a few examples. In this study, we developed a novel in situ hybridization assay for human papillomavirus (HPV) E6/...

Descripción completa

Detalles Bibliográficos
Autores principales:	Rao, Xuelian, Zheng, Liangkai, Wei, Kaipeng, Li, Meiqing, Jiang, Meng, Qiu, Jianlong, Zhou, Yulin, Ke, Rongqin, Lin, Chen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2023
Materias:	Methods and Protocols
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10101027/ https://www.ncbi.nlm.nih.gov/pubmed/36809088 http://dx.doi.org/10.1128/spectrum.03896-22

Ejemplares similares

Single molecule chromogenic in situ hybridization assay for RNA visualization in fixed cells and tissues
por: Jiang, Meng, et al.
Publicado: (2019)

Validation of a DKK1 RNAscope chromogenic in situ hybridization assay for gastric and gastroesophageal junction adenocarcinoma tumors
por: Caldwell, Charles, et al.
Publicado: (2021)

Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing
por: Poulsen, Tim S., et al.
Publicado: (2013)

Combined Fluorescent-Chromogenic In Situ Hybridization for Identification and Laser Microdissection of Interphase Chromosomes
por: Paz, Nerea, et al.
Publicado: (2013)

Optimizing Ventana chromogenic dual in-situ hybridization for mucinous epithelial ovarian cancer
por: Li, Xinyun, et al.
Publicado: (2013)

Monosomy 3 by chromogenic in situ hybridization (CISH) in Iranian patients with uveal melanoma
por: Naseripour, Masood, et al.
Publicado: (2015)

Chromogenic in situ hybridization technique for detecting porcine circovirus 3 in lung and lymphoid tissues
por: Tan, Chew Yee, et al.
Publicado: (2023)

Identification of antisense nucleic acid hybridization sites in mRNA molecules with self-quenching fluorescent reporter molecules
por: Gifford, Lida K., et al.
Publicado: (2005)

Chromogenic In Situ Hybridization and p16/Ki67 Dual Staining on Formalin-Fixed Paraffin-Embedded Cervical Specimens: Correlation with HPV-DNA Test, E6/E7 mRNA Test, and Potential Clinical Applications
por: Zappacosta, Roberta, et al.
Publicado: (2013)

Detection of Epstein Barr Virus by Chromogenic In Situ Hybridization in cases of extra-hepatic biliary atresia
por: Mahjoub, Fatemeh, et al.
Publicado: (2008)

Detection of Tritrichomonas foetus and Pentatrichomonas hominis in intestinal tissue specimens of cats by chromogenic in situ hybridization
por: Mostegl, Meike M., et al.
Publicado: (2012)

Molecular probes for the identification of avian Haemoproteus and Leucocytozoon parasites in tissue sections by chromogenic in situ hybridization
por: Himmel, Tanja, et al.
Publicado: (2019)

Chromogenic in situ hybridization compared with other approaches to evaluate HER2/neu status in breast carcinomas
por: Rosa, F.E., et al.
Publicado: (2013)

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
por: Hauptmann, Giselbert, et al.
Publicado: (2016)

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
por: Blanco, Ana M., et al.
Publicado: (2009)

dotdotdot: an automated approach to quantify multiplex single molecule fluorescent in situ hybridization (smFISH) images in complex tissues
por: Maynard, Kristen R, et al.
Publicado: (2020)

HPV E6/E7 mRNA in situ hybridization in endocervical adenocarcinoma: implications for prognosis and diagnosis
por: Luo, Rong-Zhen, et al.
Publicado: (2021)

Quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data
por: Lee, Chang-Kyu, et al.
Publicado: (2008)

Fluorescently-tagged human eIF3 for single-molecule spectroscopy
por: Johnson, Alex G, et al.
Publicado: (2018)

E-Predict: a computational strategy for species identification based on observed DNA microarray hybridization patterns
por: Urisman, Anatoly, et al.
Publicado: (2005)

Correlation of AR, EGFR, and HER2 Expression Levels in Prostate Cancer: Immunohistochemical Analysis and Chromogenic In Situ Hybridization
por: Baek, Kwang Hyun, et al.
Publicado: (2012)

Establishing a protocol for immunocytochemical staining and chromogenic in situ hybridization of Giemsa and Diff-Quick prestained cytological smears
por: Beraki, Elsa, et al.
Publicado: (2012)

A whole-mount in situ hybridization method for microRNA detection in Caenorhabditis elegans
por: Andachi, Yoshiki, et al.
Publicado: (2016)

RNAscope in situ hybridization-based method for detecting DUX4 RNA expression in vitro
por: Amini Chermahini, Gholamhossein, et al.
Publicado: (2019)

The Expression of HPV E6/E7 mRNA In Situ Hybridization in HPV Typing-negative Cervical Cancer
por: Xu, Yating, et al.
Publicado: (2023)

Dual color chromogenic in situ hybridization for determination of HER2 status in breast cancer: a large comparative study to current state of the art fluorescence in situ hybridization
por: Mollerup, Jens, et al.
Publicado: (2012)

MiL-FISH: Multilabeled Oligonucleotides for Fluorescence In Situ Hybridization Improve Visualization of Bacterial Cells
por: Schimak, Mario P., et al.
Publicado: (2015)

Metaplastic breast carcinomas exhibit EGFR, but not HER2, gene amplification and overexpression: immunohistochemical and chromogenic in situ hybridization analysis
por: Reis-Filho, Jorge S, et al.
Publicado: (2005)

Proto-oncogene HER-2 in normal, dysplastic and tumorous feline mammary glands: an immunohistochemical and chromogenic in situ hybridization study
por: Ordás, Javier, et al.
Publicado: (2007)

Construction of repeat-free fluorescence in situ hybridization probes
por: Swennenhuis, Joost F., et al.
Publicado: (2012)

Performance of the Human Papillomavirus E6/E7 mRNA Assay in the Primary Screening of Cervical Cancer: Opportunistic Screening in Fujian, China
por: Zhuang, Lijuan, et al.
Publicado: (2022)

Accuracy of HPV E6/E7 mRNA examination using in situ hybridization in diagnosing cervical intraepithelial lesions
por: Hui, Chang, et al.
Publicado: (2021)

Human papillomavirus E6E7 mRNA and TERC lncRNA in situ detection in cervical scraped cells and cervical disease progression assessment
por: Zhao, Hui, et al.
Publicado: (2022)

capCLIP: a new tool to probe translational control in human cells through capture and identification of the eIF4E–mRNA interactome
por: Jensen, Kirk B, et al.
Publicado: (2021)

Minimizing off-target signals in RNA fluorescent in situ hybridization
por: Arvey, Aaron, et al.
Publicado: (2010)

Cytoplasmic DNA can be detected by RNA fluorescence in situ hybridization
por: Greenberg, Eliraz, et al.
Publicado: (2019)

Spatially multiplexed RNA in situ hybridization to reveal tumor heterogeneity
por: Voith von Voithenberg, Lena, et al.
Publicado: (2020)

Evaluation of e-liquid toxicity using an open-source high-throughput screening assay
por: Sassano, M. Flori, et al.
Publicado: (2018)

FGFR1 amplification in breast carcinomas: a chromogenic in situ hybridisation analysis
por: Elbauomy Elsheikh, Somaia, et al.
Publicado: (2007)

Chromogenic detection of telomere lengths in situ aids the identification of precancerous lesions in the prostate
por: Ertunc, Onur, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_7bfqjhsa16760jah26lafb7u19