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Role of CCT4/ErbB signaling in nephroblastoma: Implications for a biomarker of Wilms tumor

Wilms tumor is a common abdominal malignant tumor in children. However, the molecular mechanism of Wilms tumor is unclear. GSE66405 and GSE197047 were obtained from the Gene Expression Omnibus database. To identify differentially expressed genes (DEGs) in Wilms tumor, the R package “limma” was used....

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Autores principales: Wang, Haoyuan, Zhang, Lei, Liu, Bin, Su, Jianzhi, Ni, Xiaochen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10101284/
https://www.ncbi.nlm.nih.gov/pubmed/37058032
http://dx.doi.org/10.1097/MD.0000000000033219
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author Wang, Haoyuan
Zhang, Lei
Liu, Bin
Su, Jianzhi
Ni, Xiaochen
author_facet Wang, Haoyuan
Zhang, Lei
Liu, Bin
Su, Jianzhi
Ni, Xiaochen
author_sort Wang, Haoyuan
collection PubMed
description Wilms tumor is a common abdominal malignant tumor in children. However, the molecular mechanism of Wilms tumor is unclear. GSE66405 and GSE197047 were obtained from the Gene Expression Omnibus database. To identify differentially expressed genes (DEGs) in Wilms tumor, the R package “limma” was used. Weighted gene co-expression network analysis was performed to identify the significant module. The list of DEGs was input into the Search Tool for the Retrieval of Interacting Genes database to construct a protein-protein interaction network for predicting core genes. Gene Ontology analysis and the Kyoto Encyclopedia of Genes and Genomes analysis are computational methods for assessing gene function and biological pathways. The genome was analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes and developed by gene set enrichment analysis. Comparative Toxicogenomics Database analysis was performed to find the diseases most related to the core genes. TargetScan was used to screen for miRNAs that regulate hub genes. A total of 925 DEGs were identified. The differently expressed genes were mainly enriched in the metabolic pathway, AMPK signaling pathway, ErbB signaling pathway, mRNA detection pathway, and folded protein binding. A total of 16 core genes (HNRNPK, PABPC1, HNRNPD, NCL, YBX1, EIF4G1, KHDRBS1, HNRNPAB, HSPA4, EEF2, HSP90AA1, EEF1A1, A TP5A1, SDHA, CCT4, CCT5) were obtained. chaperonin containing TCP-1 subunit 4 (CCT4) was downregulated in tumor tissue samples, which may have reverse regulatory significance for Wilms tumor. CCT4, HSP90AA1, NCL, PABPC1, and YBX1 were found to be associated with kidney disease, acute kidney injury, edema, tumor metastasis, transitional cell carcinoma, necrosis, and inflammation. The research found that the related miRNA of the CCT4 gene was hsamiR-7-5p. CCT4 might play an essential role in the occurrence and development of Wilms tumor, and they may participate in the occurrence and development of Wilms tumor through the ERBB signal pathway. CCT4 may be a promising biomarker of Wilms tumor.
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spelling pubmed-101012842023-04-14 Role of CCT4/ErbB signaling in nephroblastoma: Implications for a biomarker of Wilms tumor Wang, Haoyuan Zhang, Lei Liu, Bin Su, Jianzhi Ni, Xiaochen Medicine (Baltimore) 7300 Wilms tumor is a common abdominal malignant tumor in children. However, the molecular mechanism of Wilms tumor is unclear. GSE66405 and GSE197047 were obtained from the Gene Expression Omnibus database. To identify differentially expressed genes (DEGs) in Wilms tumor, the R package “limma” was used. Weighted gene co-expression network analysis was performed to identify the significant module. The list of DEGs was input into the Search Tool for the Retrieval of Interacting Genes database to construct a protein-protein interaction network for predicting core genes. Gene Ontology analysis and the Kyoto Encyclopedia of Genes and Genomes analysis are computational methods for assessing gene function and biological pathways. The genome was analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes and developed by gene set enrichment analysis. Comparative Toxicogenomics Database analysis was performed to find the diseases most related to the core genes. TargetScan was used to screen for miRNAs that regulate hub genes. A total of 925 DEGs were identified. The differently expressed genes were mainly enriched in the metabolic pathway, AMPK signaling pathway, ErbB signaling pathway, mRNA detection pathway, and folded protein binding. A total of 16 core genes (HNRNPK, PABPC1, HNRNPD, NCL, YBX1, EIF4G1, KHDRBS1, HNRNPAB, HSPA4, EEF2, HSP90AA1, EEF1A1, A TP5A1, SDHA, CCT4, CCT5) were obtained. chaperonin containing TCP-1 subunit 4 (CCT4) was downregulated in tumor tissue samples, which may have reverse regulatory significance for Wilms tumor. CCT4, HSP90AA1, NCL, PABPC1, and YBX1 were found to be associated with kidney disease, acute kidney injury, edema, tumor metastasis, transitional cell carcinoma, necrosis, and inflammation. The research found that the related miRNA of the CCT4 gene was hsamiR-7-5p. CCT4 might play an essential role in the occurrence and development of Wilms tumor, and they may participate in the occurrence and development of Wilms tumor through the ERBB signal pathway. CCT4 may be a promising biomarker of Wilms tumor. Lippincott Williams & Wilkins 2023-04-14 /pmc/articles/PMC10101284/ /pubmed/37058032 http://dx.doi.org/10.1097/MD.0000000000033219 Text en Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY) (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle 7300
Wang, Haoyuan
Zhang, Lei
Liu, Bin
Su, Jianzhi
Ni, Xiaochen
Role of CCT4/ErbB signaling in nephroblastoma: Implications for a biomarker of Wilms tumor
title Role of CCT4/ErbB signaling in nephroblastoma: Implications for a biomarker of Wilms tumor
title_full Role of CCT4/ErbB signaling in nephroblastoma: Implications for a biomarker of Wilms tumor
title_fullStr Role of CCT4/ErbB signaling in nephroblastoma: Implications for a biomarker of Wilms tumor
title_full_unstemmed Role of CCT4/ErbB signaling in nephroblastoma: Implications for a biomarker of Wilms tumor
title_short Role of CCT4/ErbB signaling in nephroblastoma: Implications for a biomarker of Wilms tumor
title_sort role of cct4/erbb signaling in nephroblastoma: implications for a biomarker of wilms tumor
topic 7300
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10101284/
https://www.ncbi.nlm.nih.gov/pubmed/37058032
http://dx.doi.org/10.1097/MD.0000000000033219
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