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Condyloma acuminata: An evaluation of the immune response at cellular and molecular levels

Condyloma acuminata (CA) is a benign proliferative disease mainly affecting in non-keratinized epithelia. Most cases of CA are caused by low-risk human papillomavirus (HPV), mainly HPV 6 and 11. The aim of the current study was to highlight the candidate genes and pathways associated with immune alt...

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Autores principales: Stuqui, Bruna, Provazzi, Paola Jocelan Scarin, Lima, Maria Leticia Duarte, Cabral, Ágata Silva, Leonel, Ellen Cristina Rivas, Candido, Natalia Maria, Taboga, Sebastião Roberto, da Silva, Márcia Guimarães, Lima, Flávio de Oliveira, Melli, Patrícia Pereira dos Santos, Quintana, Silvana Maria, Calmon, Marilia de Freitas, Rahal, Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10101375/
https://www.ncbi.nlm.nih.gov/pubmed/37053156
http://dx.doi.org/10.1371/journal.pone.0284296
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author Stuqui, Bruna
Provazzi, Paola Jocelan Scarin
Lima, Maria Leticia Duarte
Cabral, Ágata Silva
Leonel, Ellen Cristina Rivas
Candido, Natalia Maria
Taboga, Sebastião Roberto
da Silva, Márcia Guimarães
Lima, Flávio de Oliveira
Melli, Patrícia Pereira dos Santos
Quintana, Silvana Maria
Calmon, Marilia de Freitas
Rahal, Paula
author_facet Stuqui, Bruna
Provazzi, Paola Jocelan Scarin
Lima, Maria Leticia Duarte
Cabral, Ágata Silva
Leonel, Ellen Cristina Rivas
Candido, Natalia Maria
Taboga, Sebastião Roberto
da Silva, Márcia Guimarães
Lima, Flávio de Oliveira
Melli, Patrícia Pereira dos Santos
Quintana, Silvana Maria
Calmon, Marilia de Freitas
Rahal, Paula
author_sort Stuqui, Bruna
collection PubMed
description Condyloma acuminata (CA) is a benign proliferative disease mainly affecting in non-keratinized epithelia. Most cases of CA are caused by low-risk human papillomavirus (HPV), mainly HPV 6 and 11. The aim of the current study was to highlight the candidate genes and pathways associated with immune alterations in individuals who did not spontaneously eliminate the virus and, thus, develop genital warts. Paraffin-embedded condyloma samples (n = 56) were analyzed by immunohistochemistry using antibodies against CD1a, FOXP3, CD3, CD4, CD8, and IFN-γ. The immunomarkers were chosen based on the evaluation of the innate and adaptive immune pathways using qPCR analysis of 92 immune-related genes, applying a TaqMan Array Immune Response assay in HPV 6 or HPV 11 positive samples (n = 27). Gene expression analysis revealed 31 differentially expressed genes in CA lesions. Gene expression validation revealed upregulation of GZMB, IFNG, IL12B, and IL8 and downregulation of NFATC4 and IL7 in CA samples. Immunohistochemical analysis showed increased FOXP3, IFN-γ, CD1a, and CD4 expression in CA than in the control tissue samples. In contrast, CD3 and CD8 expression was decreased in CA lesion samples. Increased levels of pro-inflammatory cytokines in HPV-positive patients compared with HPV-negative patients seem to reflect the elevated immunogenicity of HPV-positive CA lesions. Host defense against HPV begins during the early stages of the innate immune response and is followed by activation of T lymphocytes, which are mainly represented by CD4+ and regulatory T cells. The low CD8+ T cell count in CA may contribute to this recurrent behavior. Additional studies are needed to elucidate the mechanism of host defense against HPV infection in CA.
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spelling pubmed-101013752023-04-14 Condyloma acuminata: An evaluation of the immune response at cellular and molecular levels Stuqui, Bruna Provazzi, Paola Jocelan Scarin Lima, Maria Leticia Duarte Cabral, Ágata Silva Leonel, Ellen Cristina Rivas Candido, Natalia Maria Taboga, Sebastião Roberto da Silva, Márcia Guimarães Lima, Flávio de Oliveira Melli, Patrícia Pereira dos Santos Quintana, Silvana Maria Calmon, Marilia de Freitas Rahal, Paula PLoS One Research Article Condyloma acuminata (CA) is a benign proliferative disease mainly affecting in non-keratinized epithelia. Most cases of CA are caused by low-risk human papillomavirus (HPV), mainly HPV 6 and 11. The aim of the current study was to highlight the candidate genes and pathways associated with immune alterations in individuals who did not spontaneously eliminate the virus and, thus, develop genital warts. Paraffin-embedded condyloma samples (n = 56) were analyzed by immunohistochemistry using antibodies against CD1a, FOXP3, CD3, CD4, CD8, and IFN-γ. The immunomarkers were chosen based on the evaluation of the innate and adaptive immune pathways using qPCR analysis of 92 immune-related genes, applying a TaqMan Array Immune Response assay in HPV 6 or HPV 11 positive samples (n = 27). Gene expression analysis revealed 31 differentially expressed genes in CA lesions. Gene expression validation revealed upregulation of GZMB, IFNG, IL12B, and IL8 and downregulation of NFATC4 and IL7 in CA samples. Immunohistochemical analysis showed increased FOXP3, IFN-γ, CD1a, and CD4 expression in CA than in the control tissue samples. In contrast, CD3 and CD8 expression was decreased in CA lesion samples. Increased levels of pro-inflammatory cytokines in HPV-positive patients compared with HPV-negative patients seem to reflect the elevated immunogenicity of HPV-positive CA lesions. Host defense against HPV begins during the early stages of the innate immune response and is followed by activation of T lymphocytes, which are mainly represented by CD4+ and regulatory T cells. The low CD8+ T cell count in CA may contribute to this recurrent behavior. Additional studies are needed to elucidate the mechanism of host defense against HPV infection in CA. Public Library of Science 2023-04-13 /pmc/articles/PMC10101375/ /pubmed/37053156 http://dx.doi.org/10.1371/journal.pone.0284296 Text en © 2023 Stuqui et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Stuqui, Bruna
Provazzi, Paola Jocelan Scarin
Lima, Maria Leticia Duarte
Cabral, Ágata Silva
Leonel, Ellen Cristina Rivas
Candido, Natalia Maria
Taboga, Sebastião Roberto
da Silva, Márcia Guimarães
Lima, Flávio de Oliveira
Melli, Patrícia Pereira dos Santos
Quintana, Silvana Maria
Calmon, Marilia de Freitas
Rahal, Paula
Condyloma acuminata: An evaluation of the immune response at cellular and molecular levels
title Condyloma acuminata: An evaluation of the immune response at cellular and molecular levels
title_full Condyloma acuminata: An evaluation of the immune response at cellular and molecular levels
title_fullStr Condyloma acuminata: An evaluation of the immune response at cellular and molecular levels
title_full_unstemmed Condyloma acuminata: An evaluation of the immune response at cellular and molecular levels
title_short Condyloma acuminata: An evaluation of the immune response at cellular and molecular levels
title_sort condyloma acuminata: an evaluation of the immune response at cellular and molecular levels
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10101375/
https://www.ncbi.nlm.nih.gov/pubmed/37053156
http://dx.doi.org/10.1371/journal.pone.0284296
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