A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions

Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode Caenorhabditis elegans contains an expanded family of 19 functional AGO proteins, many of which have not been fully characterized. In this work, we systematically analyzed...

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Autores principales: Seroussi, Uri, Lugowski, Andrew, Wadi, Lina, Lao, Robert X, Willis, Alexandra R, Zhao, Winnie, Sundby, Adam E, Charlesworth, Amanda G, Reinke, Aaron W, Claycomb, Julie M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10101689/
https://www.ncbi.nlm.nih.gov/pubmed/36790166
http://dx.doi.org/10.7554/eLife.83853
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author Seroussi, Uri
Lugowski, Andrew
Wadi, Lina
Lao, Robert X
Willis, Alexandra R
Zhao, Winnie
Sundby, Adam E
Charlesworth, Amanda G
Reinke, Aaron W
Claycomb, Julie M
author_facet Seroussi, Uri
Lugowski, Andrew
Wadi, Lina
Lao, Robert X
Willis, Alexandra R
Zhao, Winnie
Sundby, Adam E
Charlesworth, Amanda G
Reinke, Aaron W
Claycomb, Julie M
author_sort Seroussi, Uri
collection PubMed
description Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode Caenorhabditis elegans contains an expanded family of 19 functional AGO proteins, many of which have not been fully characterized. In this work, we systematically analyzed every C. elegans AGO using CRISPR-Cas9 genome editing to introduce GFP::3xFLAG tags. We have characterized the expression patterns of each AGO throughout development, identified small RNA binding complements, and determined the effects of ago loss on small RNA populations and developmental phenotypes. Our analysis indicates stratification of subsets of AGOs into distinct regulatory modules, and integration of our data led us to uncover novel stress-induced fertility and pathogen response phenotypes due to ago loss.
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spelling pubmed-101016892023-04-14 A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions Seroussi, Uri Lugowski, Andrew Wadi, Lina Lao, Robert X Willis, Alexandra R Zhao, Winnie Sundby, Adam E Charlesworth, Amanda G Reinke, Aaron W Claycomb, Julie M eLife Developmental Biology Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode Caenorhabditis elegans contains an expanded family of 19 functional AGO proteins, many of which have not been fully characterized. In this work, we systematically analyzed every C. elegans AGO using CRISPR-Cas9 genome editing to introduce GFP::3xFLAG tags. We have characterized the expression patterns of each AGO throughout development, identified small RNA binding complements, and determined the effects of ago loss on small RNA populations and developmental phenotypes. Our analysis indicates stratification of subsets of AGOs into distinct regulatory modules, and integration of our data led us to uncover novel stress-induced fertility and pathogen response phenotypes due to ago loss. eLife Sciences Publications, Ltd 2023-02-15 /pmc/articles/PMC10101689/ /pubmed/36790166 http://dx.doi.org/10.7554/eLife.83853 Text en © 2023, Seroussi et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Seroussi, Uri
Lugowski, Andrew
Wadi, Lina
Lao, Robert X
Willis, Alexandra R
Zhao, Winnie
Sundby, Adam E
Charlesworth, Amanda G
Reinke, Aaron W
Claycomb, Julie M
A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions
title A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions
title_full A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions
title_fullStr A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions
title_full_unstemmed A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions
title_short A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions
title_sort comprehensive survey of c. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10101689/
https://www.ncbi.nlm.nih.gov/pubmed/36790166
http://dx.doi.org/10.7554/eLife.83853
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