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Mechanical stimulation controls osteoclast function through the regulation of Ca(2+)-activated Cl(−) channel Anoctamin 1
Mechanical force loading is essential for maintaining bone homeostasis, and unloading exposure can lead to bone loss. Osteoclasts are the only bone resorbing cells and play a crucial role in bone remodeling. The molecular mechanisms underlying mechanical stimulation-induced changes in osteoclast fun...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102170/ https://www.ncbi.nlm.nih.gov/pubmed/37055517 http://dx.doi.org/10.1038/s42003-023-04806-1 |
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author | Sun, Weijia Li, Yuheng Li, Jianwei Tan, Yingjun Yuan, Xinxin Meng, Haoye Ye, Jianting Zhong, Guohui Jin, XiaoYan Liu, Zizhong Du, Ruikai Xing, Wenjuan Zhao, Dingsheng Song, Jinping Li, Youyou Pan, Junjie Zhao, Yunzhang Li, Qi Wang, Aiyuan Ling, Shukuan Dai, Rongji Li, Yingxian |
author_facet | Sun, Weijia Li, Yuheng Li, Jianwei Tan, Yingjun Yuan, Xinxin Meng, Haoye Ye, Jianting Zhong, Guohui Jin, XiaoYan Liu, Zizhong Du, Ruikai Xing, Wenjuan Zhao, Dingsheng Song, Jinping Li, Youyou Pan, Junjie Zhao, Yunzhang Li, Qi Wang, Aiyuan Ling, Shukuan Dai, Rongji Li, Yingxian |
author_sort | Sun, Weijia |
collection | PubMed |
description | Mechanical force loading is essential for maintaining bone homeostasis, and unloading exposure can lead to bone loss. Osteoclasts are the only bone resorbing cells and play a crucial role in bone remodeling. The molecular mechanisms underlying mechanical stimulation-induced changes in osteoclast function remain to be fully elucidated. Our previous research found Ca(2+)-activated Cl(−) channel Anoctamin 1 (Ano1) was an essential regulator for osteoclast function. Here, we report that Ano1 mediates osteoclast responses to mechanical stimulation. In vitro, osteoclast activities are obviously affected by mechanical stress, which is accompanied by the changes of Ano1 levels, intracellular Cl(−) concentration and Ca(2+) downstream signaling. Ano1 knockout or calcium binding mutants blunts the response of osteoclast to mechanical stimulation. In vivo, Ano1 knockout in osteoclast blunts loading induced osteoclast inhibition and unloading induced bone loss and. These results demonstrate that Ano1 plays an important role in mechanical stimulation induced osteoclast activity changes. |
format | Online Article Text |
id | pubmed-10102170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101021702023-04-15 Mechanical stimulation controls osteoclast function through the regulation of Ca(2+)-activated Cl(−) channel Anoctamin 1 Sun, Weijia Li, Yuheng Li, Jianwei Tan, Yingjun Yuan, Xinxin Meng, Haoye Ye, Jianting Zhong, Guohui Jin, XiaoYan Liu, Zizhong Du, Ruikai Xing, Wenjuan Zhao, Dingsheng Song, Jinping Li, Youyou Pan, Junjie Zhao, Yunzhang Li, Qi Wang, Aiyuan Ling, Shukuan Dai, Rongji Li, Yingxian Commun Biol Article Mechanical force loading is essential for maintaining bone homeostasis, and unloading exposure can lead to bone loss. Osteoclasts are the only bone resorbing cells and play a crucial role in bone remodeling. The molecular mechanisms underlying mechanical stimulation-induced changes in osteoclast function remain to be fully elucidated. Our previous research found Ca(2+)-activated Cl(−) channel Anoctamin 1 (Ano1) was an essential regulator for osteoclast function. Here, we report that Ano1 mediates osteoclast responses to mechanical stimulation. In vitro, osteoclast activities are obviously affected by mechanical stress, which is accompanied by the changes of Ano1 levels, intracellular Cl(−) concentration and Ca(2+) downstream signaling. Ano1 knockout or calcium binding mutants blunts the response of osteoclast to mechanical stimulation. In vivo, Ano1 knockout in osteoclast blunts loading induced osteoclast inhibition and unloading induced bone loss and. These results demonstrate that Ano1 plays an important role in mechanical stimulation induced osteoclast activity changes. Nature Publishing Group UK 2023-04-13 /pmc/articles/PMC10102170/ /pubmed/37055517 http://dx.doi.org/10.1038/s42003-023-04806-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Sun, Weijia Li, Yuheng Li, Jianwei Tan, Yingjun Yuan, Xinxin Meng, Haoye Ye, Jianting Zhong, Guohui Jin, XiaoYan Liu, Zizhong Du, Ruikai Xing, Wenjuan Zhao, Dingsheng Song, Jinping Li, Youyou Pan, Junjie Zhao, Yunzhang Li, Qi Wang, Aiyuan Ling, Shukuan Dai, Rongji Li, Yingxian Mechanical stimulation controls osteoclast function through the regulation of Ca(2+)-activated Cl(−) channel Anoctamin 1 |
title | Mechanical stimulation controls osteoclast function through the regulation of Ca(2+)-activated Cl(−) channel Anoctamin 1 |
title_full | Mechanical stimulation controls osteoclast function through the regulation of Ca(2+)-activated Cl(−) channel Anoctamin 1 |
title_fullStr | Mechanical stimulation controls osteoclast function through the regulation of Ca(2+)-activated Cl(−) channel Anoctamin 1 |
title_full_unstemmed | Mechanical stimulation controls osteoclast function through the regulation of Ca(2+)-activated Cl(−) channel Anoctamin 1 |
title_short | Mechanical stimulation controls osteoclast function through the regulation of Ca(2+)-activated Cl(−) channel Anoctamin 1 |
title_sort | mechanical stimulation controls osteoclast function through the regulation of ca(2+)-activated cl(−) channel anoctamin 1 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102170/ https://www.ncbi.nlm.nih.gov/pubmed/37055517 http://dx.doi.org/10.1038/s42003-023-04806-1 |
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