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Integrated model of the vertebrate augmin complex

Accurate segregation of chromosomes is required to maintain genome integrity during cell division. This feat is accomplished by the microtubule-based spindle. To build a spindle rapidly and with high fidelity, cells take advantage of branching microtubule nucleation, which rapidly amplifies microtub...

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Autores principales: Travis, Sophie M., Mahon, Brian P., Huang, Wei, Ma, Meisheng, Rale, Michael J., Kraus, Jodi, Taylor, Derek J., Zhang, Rui, Petry, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102177/
https://www.ncbi.nlm.nih.gov/pubmed/37055408
http://dx.doi.org/10.1038/s41467-023-37519-4
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author Travis, Sophie M.
Mahon, Brian P.
Huang, Wei
Ma, Meisheng
Rale, Michael J.
Kraus, Jodi
Taylor, Derek J.
Zhang, Rui
Petry, Sabine
author_facet Travis, Sophie M.
Mahon, Brian P.
Huang, Wei
Ma, Meisheng
Rale, Michael J.
Kraus, Jodi
Taylor, Derek J.
Zhang, Rui
Petry, Sabine
author_sort Travis, Sophie M.
collection PubMed
description Accurate segregation of chromosomes is required to maintain genome integrity during cell division. This feat is accomplished by the microtubule-based spindle. To build a spindle rapidly and with high fidelity, cells take advantage of branching microtubule nucleation, which rapidly amplifies microtubules during cell division. Branching microtubule nucleation relies on the hetero-octameric augmin complex, but lack of structure information about augmin has hindered understanding how it promotes branching. In this work, we combine cryo-electron microscopy, protein structural prediction, and visualization of fused bulky tags via negative stain electron microscopy to identify the location and orientation of each subunit within the augmin structure. Evolutionary analysis shows that augmin’s structure is highly conserved across eukaryotes, and that augmin contains a previously unidentified microtubule binding site. Thus, our findings provide insight into the mechanism of branching microtubule nucleation.
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spelling pubmed-101021772023-04-15 Integrated model of the vertebrate augmin complex Travis, Sophie M. Mahon, Brian P. Huang, Wei Ma, Meisheng Rale, Michael J. Kraus, Jodi Taylor, Derek J. Zhang, Rui Petry, Sabine Nat Commun Article Accurate segregation of chromosomes is required to maintain genome integrity during cell division. This feat is accomplished by the microtubule-based spindle. To build a spindle rapidly and with high fidelity, cells take advantage of branching microtubule nucleation, which rapidly amplifies microtubules during cell division. Branching microtubule nucleation relies on the hetero-octameric augmin complex, but lack of structure information about augmin has hindered understanding how it promotes branching. In this work, we combine cryo-electron microscopy, protein structural prediction, and visualization of fused bulky tags via negative stain electron microscopy to identify the location and orientation of each subunit within the augmin structure. Evolutionary analysis shows that augmin’s structure is highly conserved across eukaryotes, and that augmin contains a previously unidentified microtubule binding site. Thus, our findings provide insight into the mechanism of branching microtubule nucleation. Nature Publishing Group UK 2023-04-13 /pmc/articles/PMC10102177/ /pubmed/37055408 http://dx.doi.org/10.1038/s41467-023-37519-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Travis, Sophie M.
Mahon, Brian P.
Huang, Wei
Ma, Meisheng
Rale, Michael J.
Kraus, Jodi
Taylor, Derek J.
Zhang, Rui
Petry, Sabine
Integrated model of the vertebrate augmin complex
title Integrated model of the vertebrate augmin complex
title_full Integrated model of the vertebrate augmin complex
title_fullStr Integrated model of the vertebrate augmin complex
title_full_unstemmed Integrated model of the vertebrate augmin complex
title_short Integrated model of the vertebrate augmin complex
title_sort integrated model of the vertebrate augmin complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102177/
https://www.ncbi.nlm.nih.gov/pubmed/37055408
http://dx.doi.org/10.1038/s41467-023-37519-4
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