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Integrated model of the vertebrate augmin complex
Accurate segregation of chromosomes is required to maintain genome integrity during cell division. This feat is accomplished by the microtubule-based spindle. To build a spindle rapidly and with high fidelity, cells take advantage of branching microtubule nucleation, which rapidly amplifies microtub...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102177/ https://www.ncbi.nlm.nih.gov/pubmed/37055408 http://dx.doi.org/10.1038/s41467-023-37519-4 |
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author | Travis, Sophie M. Mahon, Brian P. Huang, Wei Ma, Meisheng Rale, Michael J. Kraus, Jodi Taylor, Derek J. Zhang, Rui Petry, Sabine |
author_facet | Travis, Sophie M. Mahon, Brian P. Huang, Wei Ma, Meisheng Rale, Michael J. Kraus, Jodi Taylor, Derek J. Zhang, Rui Petry, Sabine |
author_sort | Travis, Sophie M. |
collection | PubMed |
description | Accurate segregation of chromosomes is required to maintain genome integrity during cell division. This feat is accomplished by the microtubule-based spindle. To build a spindle rapidly and with high fidelity, cells take advantage of branching microtubule nucleation, which rapidly amplifies microtubules during cell division. Branching microtubule nucleation relies on the hetero-octameric augmin complex, but lack of structure information about augmin has hindered understanding how it promotes branching. In this work, we combine cryo-electron microscopy, protein structural prediction, and visualization of fused bulky tags via negative stain electron microscopy to identify the location and orientation of each subunit within the augmin structure. Evolutionary analysis shows that augmin’s structure is highly conserved across eukaryotes, and that augmin contains a previously unidentified microtubule binding site. Thus, our findings provide insight into the mechanism of branching microtubule nucleation. |
format | Online Article Text |
id | pubmed-10102177 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101021772023-04-15 Integrated model of the vertebrate augmin complex Travis, Sophie M. Mahon, Brian P. Huang, Wei Ma, Meisheng Rale, Michael J. Kraus, Jodi Taylor, Derek J. Zhang, Rui Petry, Sabine Nat Commun Article Accurate segregation of chromosomes is required to maintain genome integrity during cell division. This feat is accomplished by the microtubule-based spindle. To build a spindle rapidly and with high fidelity, cells take advantage of branching microtubule nucleation, which rapidly amplifies microtubules during cell division. Branching microtubule nucleation relies on the hetero-octameric augmin complex, but lack of structure information about augmin has hindered understanding how it promotes branching. In this work, we combine cryo-electron microscopy, protein structural prediction, and visualization of fused bulky tags via negative stain electron microscopy to identify the location and orientation of each subunit within the augmin structure. Evolutionary analysis shows that augmin’s structure is highly conserved across eukaryotes, and that augmin contains a previously unidentified microtubule binding site. Thus, our findings provide insight into the mechanism of branching microtubule nucleation. Nature Publishing Group UK 2023-04-13 /pmc/articles/PMC10102177/ /pubmed/37055408 http://dx.doi.org/10.1038/s41467-023-37519-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Travis, Sophie M. Mahon, Brian P. Huang, Wei Ma, Meisheng Rale, Michael J. Kraus, Jodi Taylor, Derek J. Zhang, Rui Petry, Sabine Integrated model of the vertebrate augmin complex |
title | Integrated model of the vertebrate augmin complex |
title_full | Integrated model of the vertebrate augmin complex |
title_fullStr | Integrated model of the vertebrate augmin complex |
title_full_unstemmed | Integrated model of the vertebrate augmin complex |
title_short | Integrated model of the vertebrate augmin complex |
title_sort | integrated model of the vertebrate augmin complex |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102177/ https://www.ncbi.nlm.nih.gov/pubmed/37055408 http://dx.doi.org/10.1038/s41467-023-37519-4 |
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