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Fabrication of spectroscopic microfluidic chips for mastitis detection in raw milk

Mastitis is a disease that directly affects the quantity and quality of milk produced by dairy cows, which can have a negative impact on the income generated from selling the milk. Severe inflammation caused by this mammary disease can result in up to 1 × 10(6) white blood cells per milliliter of co...

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Autores principales: Phiphattanaphiphop, Chalinee, Leksakul, Komgrit, Nakkiew, Wasawat, Phatthanakun, Rungrueang, Khamlor, Trisadee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102200/
https://www.ncbi.nlm.nih.gov/pubmed/37055481
http://dx.doi.org/10.1038/s41598-023-33258-0
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author Phiphattanaphiphop, Chalinee
Leksakul, Komgrit
Nakkiew, Wasawat
Phatthanakun, Rungrueang
Khamlor, Trisadee
author_facet Phiphattanaphiphop, Chalinee
Leksakul, Komgrit
Nakkiew, Wasawat
Phatthanakun, Rungrueang
Khamlor, Trisadee
author_sort Phiphattanaphiphop, Chalinee
collection PubMed
description Mastitis is a disease that directly affects the quantity and quality of milk produced by dairy cows, which can have a negative impact on the income generated from selling the milk. Severe inflammation caused by this mammary disease can result in up to 1 × 10(6) white blood cells per milliliter of cow milk. Currently, the California mastitis test is a popular chemical inspection test, but its error rate of over 40% is a significant factor in the ongoing spread of mastitis. In this study, a new microfluidic device was designed and fabricated to identify normal, sub-clinical, and clinical mastitis. This portable device allows for precise and analysis of results within a second. The device was designed to screen somatic cells and a staining process was added to identify somatic cells using single-cell process analysis. The fluorescence principle was used to identify the infection status of the milk, which was analyzed using a mini-spectrometer. The accuracy of the device was tested, and it was found to determine the infection status with 95% accuracy, compared to the accuracy obtained using the Fossomatic machine. By introducing this new microfluidic device, it is believed that the spread of mastitis in dairy cows can be significantly reduced, leading to higher quality and more profitable milk production.
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spelling pubmed-101022002023-04-15 Fabrication of spectroscopic microfluidic chips for mastitis detection in raw milk Phiphattanaphiphop, Chalinee Leksakul, Komgrit Nakkiew, Wasawat Phatthanakun, Rungrueang Khamlor, Trisadee Sci Rep Article Mastitis is a disease that directly affects the quantity and quality of milk produced by dairy cows, which can have a negative impact on the income generated from selling the milk. Severe inflammation caused by this mammary disease can result in up to 1 × 10(6) white blood cells per milliliter of cow milk. Currently, the California mastitis test is a popular chemical inspection test, but its error rate of over 40% is a significant factor in the ongoing spread of mastitis. In this study, a new microfluidic device was designed and fabricated to identify normal, sub-clinical, and clinical mastitis. This portable device allows for precise and analysis of results within a second. The device was designed to screen somatic cells and a staining process was added to identify somatic cells using single-cell process analysis. The fluorescence principle was used to identify the infection status of the milk, which was analyzed using a mini-spectrometer. The accuracy of the device was tested, and it was found to determine the infection status with 95% accuracy, compared to the accuracy obtained using the Fossomatic machine. By introducing this new microfluidic device, it is believed that the spread of mastitis in dairy cows can be significantly reduced, leading to higher quality and more profitable milk production. Nature Publishing Group UK 2023-04-13 /pmc/articles/PMC10102200/ /pubmed/37055481 http://dx.doi.org/10.1038/s41598-023-33258-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Phiphattanaphiphop, Chalinee
Leksakul, Komgrit
Nakkiew, Wasawat
Phatthanakun, Rungrueang
Khamlor, Trisadee
Fabrication of spectroscopic microfluidic chips for mastitis detection in raw milk
title Fabrication of spectroscopic microfluidic chips for mastitis detection in raw milk
title_full Fabrication of spectroscopic microfluidic chips for mastitis detection in raw milk
title_fullStr Fabrication of spectroscopic microfluidic chips for mastitis detection in raw milk
title_full_unstemmed Fabrication of spectroscopic microfluidic chips for mastitis detection in raw milk
title_short Fabrication of spectroscopic microfluidic chips for mastitis detection in raw milk
title_sort fabrication of spectroscopic microfluidic chips for mastitis detection in raw milk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102200/
https://www.ncbi.nlm.nih.gov/pubmed/37055481
http://dx.doi.org/10.1038/s41598-023-33258-0
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