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Integrated metabolomics and lipidomics analyses suggest the temperature-dependent lipid desaturation promotes aflatoxin biosynthesis in Aspergillus flavus

Temperature is one of the main factors affecting aflatoxin (AF) biosynthesis in Aspergillus flavus. Previous studies showed that AF biosynthesis is elevated in A. flavus at temperatures between 28°C-30°C, while it is inhibited at temperatures above 30°C. However, little is known about the metabolic...

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Autores principales: Wu, Shaowen, Huang, Wenjie, Wang, Fenghua, Zou, Xinlu, Li, Xuan, Liu, Chun-Ming, Zhang, Wenyang, Yan, Shijuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102665/
https://www.ncbi.nlm.nih.gov/pubmed/37065116
http://dx.doi.org/10.3389/fmicb.2023.1137643
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author Wu, Shaowen
Huang, Wenjie
Wang, Fenghua
Zou, Xinlu
Li, Xuan
Liu, Chun-Ming
Zhang, Wenyang
Yan, Shijuan
author_facet Wu, Shaowen
Huang, Wenjie
Wang, Fenghua
Zou, Xinlu
Li, Xuan
Liu, Chun-Ming
Zhang, Wenyang
Yan, Shijuan
author_sort Wu, Shaowen
collection PubMed
description Temperature is one of the main factors affecting aflatoxin (AF) biosynthesis in Aspergillus flavus. Previous studies showed that AF biosynthesis is elevated in A. flavus at temperatures between 28°C-30°C, while it is inhibited at temperatures above 30°C. However, little is known about the metabolic mechanism underlying temperature-regulated AF biosynthesis. In this study, we integrated metabolomic and lipidomic analyses to investigate the endogenous metabolism of A. flavus across 6 days of mycelia growth at 28°C (optimal AF production) and 37°C (no AF production). Results showed that both metabolite and lipid profiles were significantly altered at different temperatures. In particular, metabolites involved in carbohydrate and amino acid metabolism were up-regulated at 37°C on the second day but down-regulated from days three to six. Moreover, lipidomics and targeted fatty acids analyses of mycelia samples revealed a distinct pattern of lipid species and free fatty acids desaturation. High degrees of polyunsaturation of most lipid species at 28°C were positively correlated with AF production. These results provide new insights into the underlying metabolic changes in A. flavus under temperature stress.
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spelling pubmed-101026652023-04-15 Integrated metabolomics and lipidomics analyses suggest the temperature-dependent lipid desaturation promotes aflatoxin biosynthesis in Aspergillus flavus Wu, Shaowen Huang, Wenjie Wang, Fenghua Zou, Xinlu Li, Xuan Liu, Chun-Ming Zhang, Wenyang Yan, Shijuan Front Microbiol Microbiology Temperature is one of the main factors affecting aflatoxin (AF) biosynthesis in Aspergillus flavus. Previous studies showed that AF biosynthesis is elevated in A. flavus at temperatures between 28°C-30°C, while it is inhibited at temperatures above 30°C. However, little is known about the metabolic mechanism underlying temperature-regulated AF biosynthesis. In this study, we integrated metabolomic and lipidomic analyses to investigate the endogenous metabolism of A. flavus across 6 days of mycelia growth at 28°C (optimal AF production) and 37°C (no AF production). Results showed that both metabolite and lipid profiles were significantly altered at different temperatures. In particular, metabolites involved in carbohydrate and amino acid metabolism were up-regulated at 37°C on the second day but down-regulated from days three to six. Moreover, lipidomics and targeted fatty acids analyses of mycelia samples revealed a distinct pattern of lipid species and free fatty acids desaturation. High degrees of polyunsaturation of most lipid species at 28°C were positively correlated with AF production. These results provide new insights into the underlying metabolic changes in A. flavus under temperature stress. Frontiers Media S.A. 2023-03-31 /pmc/articles/PMC10102665/ /pubmed/37065116 http://dx.doi.org/10.3389/fmicb.2023.1137643 Text en Copyright © 2023 Wu, Huang, Wang, Zou, Li, Liu, Zhang and Yan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wu, Shaowen
Huang, Wenjie
Wang, Fenghua
Zou, Xinlu
Li, Xuan
Liu, Chun-Ming
Zhang, Wenyang
Yan, Shijuan
Integrated metabolomics and lipidomics analyses suggest the temperature-dependent lipid desaturation promotes aflatoxin biosynthesis in Aspergillus flavus
title Integrated metabolomics and lipidomics analyses suggest the temperature-dependent lipid desaturation promotes aflatoxin biosynthesis in Aspergillus flavus
title_full Integrated metabolomics and lipidomics analyses suggest the temperature-dependent lipid desaturation promotes aflatoxin biosynthesis in Aspergillus flavus
title_fullStr Integrated metabolomics and lipidomics analyses suggest the temperature-dependent lipid desaturation promotes aflatoxin biosynthesis in Aspergillus flavus
title_full_unstemmed Integrated metabolomics and lipidomics analyses suggest the temperature-dependent lipid desaturation promotes aflatoxin biosynthesis in Aspergillus flavus
title_short Integrated metabolomics and lipidomics analyses suggest the temperature-dependent lipid desaturation promotes aflatoxin biosynthesis in Aspergillus flavus
title_sort integrated metabolomics and lipidomics analyses suggest the temperature-dependent lipid desaturation promotes aflatoxin biosynthesis in aspergillus flavus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102665/
https://www.ncbi.nlm.nih.gov/pubmed/37065116
http://dx.doi.org/10.3389/fmicb.2023.1137643
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