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A Rapid, Functional sgRNA Screening Method for Generating Murine RET and NTRK1 Fusion Oncogenes

CRISPR/Cas9 gene editing technology is an indispensable and powerful tool in the field of cancer biology. To conduct successful CRISPR-based experiments, it is crucial that sgRNAs generate their designed alterations. Here, we describe a simple and efficient sgRNA screening method for validating sgRN...

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Detalles Bibliográficos
Autores principales:	Schubert, Laura, Le, Anh T., Hinz, Trista K., Navarro, Andre, Nelson-Taylor, Sarah K., Nemenoff, Raphael A., Heasley, Lynn E., Doebele, Robert C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10104125/ https://www.ncbi.nlm.nih.gov/pubmed/37066347 http://dx.doi.org/10.1101/2023.04.06.535912

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