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Plasma cell-free DNA promise monitoring and tissue injury assessment of COVID-19

Coronavirus 2019 (COVID-19) is a complex disease that affects billions of people worldwide. Currently, effective etiological treatment of COVID-19 is still lacking; COVID-19 also causes damages to various organs that affects therapeutics and mortality of the patients. Surveillance of the treatment r...

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Autores principales: Jin, Xin, Wang, Yanqun, Xu, Jinjin, Li, Yimin, Cheng, Fanjun, Luo, Yuxue, Zhou, Haibo, Lin, Shanwen, Xiao, Fei, Zhang, Lu, Lin, Yu, Zhang, Zhaoyong, Jin, Yan, Zheng, Fang, Chen, Wei, Zhu, Airu, Tao, Ye, Zhao, Jingxian, Kuo, Tingyou, Li, Yuming, Li, Lingguo, Wen, Liyan, Ou, Rijing, Li, Fang, Lin, Long, Zhang, Yanjun, Sun, Jing, Yuan, Hao, Zhuang, Zhen, Sun, Haixi, Chen, Zhao, Li, Jie, Zhuo, Jianfen, Chen, Dongsheng, Zhang, Shengnan, Sun, Yuzhe, Wei, Peilan, Yuan, Jinwei, Xu, Tian, Yang, Huanming, Wang, Jian, Xu, Xun, Zhong, Nanshan, Xu, Yonghao, Sun, Kun, Zhao, Jincun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10104435/
https://www.ncbi.nlm.nih.gov/pubmed/37059908
http://dx.doi.org/10.1007/s00438-023-02014-4
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author Jin, Xin
Wang, Yanqun
Xu, Jinjin
Li, Yimin
Cheng, Fanjun
Luo, Yuxue
Zhou, Haibo
Lin, Shanwen
Xiao, Fei
Zhang, Lu
Lin, Yu
Zhang, Zhaoyong
Jin, Yan
Zheng, Fang
Chen, Wei
Zhu, Airu
Tao, Ye
Zhao, Jingxian
Kuo, Tingyou
Li, Yuming
Li, Lingguo
Wen, Liyan
Ou, Rijing
Li, Fang
Lin, Long
Zhang, Yanjun
Sun, Jing
Yuan, Hao
Zhuang, Zhen
Sun, Haixi
Chen, Zhao
Li, Jie
Zhuo, Jianfen
Chen, Dongsheng
Zhang, Shengnan
Sun, Yuzhe
Wei, Peilan
Yuan, Jinwei
Xu, Tian
Yang, Huanming
Wang, Jian
Xu, Xun
Zhong, Nanshan
Xu, Yonghao
Sun, Kun
Zhao, Jincun
author_facet Jin, Xin
Wang, Yanqun
Xu, Jinjin
Li, Yimin
Cheng, Fanjun
Luo, Yuxue
Zhou, Haibo
Lin, Shanwen
Xiao, Fei
Zhang, Lu
Lin, Yu
Zhang, Zhaoyong
Jin, Yan
Zheng, Fang
Chen, Wei
Zhu, Airu
Tao, Ye
Zhao, Jingxian
Kuo, Tingyou
Li, Yuming
Li, Lingguo
Wen, Liyan
Ou, Rijing
Li, Fang
Lin, Long
Zhang, Yanjun
Sun, Jing
Yuan, Hao
Zhuang, Zhen
Sun, Haixi
Chen, Zhao
Li, Jie
Zhuo, Jianfen
Chen, Dongsheng
Zhang, Shengnan
Sun, Yuzhe
Wei, Peilan
Yuan, Jinwei
Xu, Tian
Yang, Huanming
Wang, Jian
Xu, Xun
Zhong, Nanshan
Xu, Yonghao
Sun, Kun
Zhao, Jincun
author_sort Jin, Xin
collection PubMed
description Coronavirus 2019 (COVID-19) is a complex disease that affects billions of people worldwide. Currently, effective etiological treatment of COVID-19 is still lacking; COVID-19 also causes damages to various organs that affects therapeutics and mortality of the patients. Surveillance of the treatment responses and organ injury assessment of COVID-19 patients are of high clinical value. In this study, we investigated the characteristic fragmentation patterns and explored the potential in tissue injury assessment of plasma cell-free DNA in COVID-19 patients. Through recruitment of 37 COVID-19 patients, 32 controls and analysis of 208 blood samples upon diagnosis and during treatment, we report gross abnormalities in cfDNA of COVID-19 patients, including elevated GC content, altered molecule size and end motif patterns. More importantly, such cfDNA fragmentation characteristics reflect patient-specific physiological changes during treatment. Further analysis on cfDNA tissue-of-origin tracing reveals frequent tissue injuries in COVID-19 patients, which is supported by clinical diagnoses. Hence, our work demonstrates and extends the translational merit of cfDNA fragmentation pattern as valuable analyte for effective treatment monitoring, as well as tissue injury assessment in COVID-19. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00438-023-02014-4.
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spelling pubmed-101044352023-04-17 Plasma cell-free DNA promise monitoring and tissue injury assessment of COVID-19 Jin, Xin Wang, Yanqun Xu, Jinjin Li, Yimin Cheng, Fanjun Luo, Yuxue Zhou, Haibo Lin, Shanwen Xiao, Fei Zhang, Lu Lin, Yu Zhang, Zhaoyong Jin, Yan Zheng, Fang Chen, Wei Zhu, Airu Tao, Ye Zhao, Jingxian Kuo, Tingyou Li, Yuming Li, Lingguo Wen, Liyan Ou, Rijing Li, Fang Lin, Long Zhang, Yanjun Sun, Jing Yuan, Hao Zhuang, Zhen Sun, Haixi Chen, Zhao Li, Jie Zhuo, Jianfen Chen, Dongsheng Zhang, Shengnan Sun, Yuzhe Wei, Peilan Yuan, Jinwei Xu, Tian Yang, Huanming Wang, Jian Xu, Xun Zhong, Nanshan Xu, Yonghao Sun, Kun Zhao, Jincun Mol Genet Genomics Original Article Coronavirus 2019 (COVID-19) is a complex disease that affects billions of people worldwide. Currently, effective etiological treatment of COVID-19 is still lacking; COVID-19 also causes damages to various organs that affects therapeutics and mortality of the patients. Surveillance of the treatment responses and organ injury assessment of COVID-19 patients are of high clinical value. In this study, we investigated the characteristic fragmentation patterns and explored the potential in tissue injury assessment of plasma cell-free DNA in COVID-19 patients. Through recruitment of 37 COVID-19 patients, 32 controls and analysis of 208 blood samples upon diagnosis and during treatment, we report gross abnormalities in cfDNA of COVID-19 patients, including elevated GC content, altered molecule size and end motif patterns. More importantly, such cfDNA fragmentation characteristics reflect patient-specific physiological changes during treatment. Further analysis on cfDNA tissue-of-origin tracing reveals frequent tissue injuries in COVID-19 patients, which is supported by clinical diagnoses. Hence, our work demonstrates and extends the translational merit of cfDNA fragmentation pattern as valuable analyte for effective treatment monitoring, as well as tissue injury assessment in COVID-19. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00438-023-02014-4. Springer Berlin Heidelberg 2023-04-14 2023 /pmc/articles/PMC10104435/ /pubmed/37059908 http://dx.doi.org/10.1007/s00438-023-02014-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Jin, Xin
Wang, Yanqun
Xu, Jinjin
Li, Yimin
Cheng, Fanjun
Luo, Yuxue
Zhou, Haibo
Lin, Shanwen
Xiao, Fei
Zhang, Lu
Lin, Yu
Zhang, Zhaoyong
Jin, Yan
Zheng, Fang
Chen, Wei
Zhu, Airu
Tao, Ye
Zhao, Jingxian
Kuo, Tingyou
Li, Yuming
Li, Lingguo
Wen, Liyan
Ou, Rijing
Li, Fang
Lin, Long
Zhang, Yanjun
Sun, Jing
Yuan, Hao
Zhuang, Zhen
Sun, Haixi
Chen, Zhao
Li, Jie
Zhuo, Jianfen
Chen, Dongsheng
Zhang, Shengnan
Sun, Yuzhe
Wei, Peilan
Yuan, Jinwei
Xu, Tian
Yang, Huanming
Wang, Jian
Xu, Xun
Zhong, Nanshan
Xu, Yonghao
Sun, Kun
Zhao, Jincun
Plasma cell-free DNA promise monitoring and tissue injury assessment of COVID-19
title Plasma cell-free DNA promise monitoring and tissue injury assessment of COVID-19
title_full Plasma cell-free DNA promise monitoring and tissue injury assessment of COVID-19
title_fullStr Plasma cell-free DNA promise monitoring and tissue injury assessment of COVID-19
title_full_unstemmed Plasma cell-free DNA promise monitoring and tissue injury assessment of COVID-19
title_short Plasma cell-free DNA promise monitoring and tissue injury assessment of COVID-19
title_sort plasma cell-free dna promise monitoring and tissue injury assessment of covid-19
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10104435/
https://www.ncbi.nlm.nih.gov/pubmed/37059908
http://dx.doi.org/10.1007/s00438-023-02014-4
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